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Author Aybar, M.; Perez-Calleja, P.; Li, M.; Pavissich, J.P.; Nerenberg, R.
Title Predation creates unique void layer in membrane-aerated biofilms Type
Year 2019 Publication Water Research Abbreviated Journal Water Res.
Volume 149 Issue Pages 232-242
Keywords MABR; Biofilm; Protozoa; Predation; Voids; Porosity
Abstract The membrane-aerated biofilm reactor (MABR) is a novel wastewater treatment technology based on oxygen-supplying membranes. The counter diffusion of oxygen and electron donors in MABRs leads to unique behavior, and we hypothesized it also could impact predation. We used optical coherence tomography (OCT), microsensor analyses, and mathematical modeling to investigate predation in membrane-aerated biofilms (MABs). When protozoa were excluded from the inoculum, the MAB's OCT-observable void fraction was around 5%. When protozoa were included, the void fraction grew to nearly 50%, with large, continuous voids at the base of the biofilm. Real-time OCT imaging showed highly motile protozoa in the voids. MABs with protozoa and a high bulk COD (270 mg/L) only had 4% void fraction. DNA sequencing revealed a high relative abundance of amoeba in both high and low-COD MABs. Flagellates were only abundant in the low-COD MAB. Modeling also suggested a relationship between substrate concentrations, diffusion mode (co- or counter-diffusion), and bioflim void fraction. Results suggest that amoeba proliferate in the bioflim interior, especially in the aerobic zones. Voids form once COD limitation at the base of MABs allows predation rates to exceed microbial growth rates. Once formed, the voids provide a niche for motile protozoa, which expand the voids into a large, continuous gap. This increases the potential for biofilm sloughing, and may have detrimental effects on slow-growing, aerobic microorganisms such as nitrifying bacteria. (C)2018 Elsevier Ltd. All rights reserved.
Address [Aybar, M.; Perez-Calleja, P.; Li, M.; Pavissich, J. P.; Nerenberg, R.] Univ Notre Dame, Dept Civil & Environm Engn & Earth Sci, 156 Fitzpatrick Hall, Notre Dame, IN 46556 USA, Email: maybar@udec.cl;
Corporate Author Thesis
Publisher Pergamon-Elsevier Science Ltd Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0043-1354 ISBN Medium
Area Expedition Conference
Notes WOS:000458221200023 Approved
Call Number UAI @ eduardo.moreno @ Serial 1166
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Author Walker, P.; Nerenberg, R.; Pizarro, G.; Aybar, M.; Pavissich, J.P.; González, B.; Pastén, P.
Title Nitrate increases the capacity of an aerobic moving-bed biofilm reactor (MBBR) for winery wastewater treatment Type
Year 2024 Publication Water Science And Technology Abbreviated Journal Water Sci. Technol.
Volume Early Access Issue Pages
Keywords biofilms; BOD removal; denitrification; kinetics; modeling
Abstract We used bench-scale tests and mathematical modeling to explore chemical oxygen demand (COD) removal rates in a moving-bed biofilm reactor (MBBR) for winery wastewater treatment, using either urea or nitrate as a nitrogen source. With urea addition, the COD removal fluxes ranged from 34 to 45 gCOD/m(2)-d. However, when nitrate was added, fluxes increased up to 65 gCOD/m(2)-d, twice the amount reported for aerobic biofilms for winery wastewater treatment. A one-dimensional biofilm model, calibrated with data from respirometric tests, accurately captured the experimental results. Both experimental and modelling results suggest that nitrate significantly increased MBBR capacity by stimulating COD oxidation in the deeper, oxygen-limited regions of the biofilm. Our research suggests that the addition of nitrate, or other energetic and broadly used electron acceptors, may provide a cost-effective means of covering peak COD loads in biofilm processes for winery or another industrial wastewater treatment.
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0273-1223 ISBN Medium
Area Expedition Conference
Notes WOS:001172605000001 Approved
Call Number UAI @ alexi.delcanto @ Serial 1957
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