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Author Dauelsberg, P.; Matus, J.T.; Poupin, M.J.; Leiva-Ampuero, A.; Godoy, F.; Vega, A.; Arce-Johnson, P.
Title (up) Effect of pollination and fertilization on the expression of genes related to floral transition, hormone synthesis and berry development in grapevine Type
Year 2011 Publication Journal Of Plant Physiology Abbreviated Journal J. Plant Physiol.
Volume 168 Issue 14 Pages 1667-1674
Keywords Anthesis; ASB1; Berry flesh; Embryo development; Flower patterning; L1L; Parthenocarpy
Abstract In the present work, the effect of assisted fertilization on anatomical, morphological and gene expression changes occurring in carpels and during early stages of berry development in Vitis vinifera were studied. Inflorescences were emasculated before capfall, immediately manually pollinated (EP) and fruit development was compared to emasculated but non-pollinated (ENP) and self-pollinated inflorescences (NESP). The diameter of berries derived from pollinated flowers (EP and NESP) was significantly higher than from non-pollinated flowers (ENP) at 21 days after emasculation/pollination (DAE), and a rapid increase in the size of the inner mesocarp, together with the presence of an embryo-like structure, were observed. The expression of gibberellin oxidases (GA200x and GA2ox), anthranilate synthase (related to auxin synthesis) and cytokinin synthase coding genes was studied to assess the relationship between hormone synthesis and early berry development, while flower patterning genes were analyzed to describe floral transition. Significant expression changes were found for hormone-related genes, suggesting that their expression at early stages of berry development (13 DAE) is related to cell division and differentiation of mesocarp tissue at a later stage (21 DAE). Expression of hormone-related genes also correlates with the expression of VvHB13, a gene related to mesocarp expansion, and with an increased repression of floral patterning genes (PISTILLATA and TM6), which may contribute to prevent floral transition inhibiting fruit growth before fertilization takes place. (C) 2011 Elsevier GmbH. All rights reserved.
Address [Dauelsberg, P; Matus, JT; Leiva-Ampuero, A; Godoy, F; Vega, A; Arce-Johnson, P] Pontificia Univ Catolica Chile, Dept Mol Genet & Microbiol, Santiago, Chile, Email: parce@bio.puc.cl
Corporate Author Thesis
Publisher Elsevier Gmbh, Urban & Fischer Verlag Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0176-1617 ISBN Medium
Area Expedition Conference
Notes WOS:000294832300008 Approved
Call Number UAI @ eduardo.moreno @ Serial 161
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