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Author De la Iglesia, R.; Valenzuela-Heredia, D.; Pavissich, J.P.; Freyhoffer, S.; Andrade, S.; Correa, J.A.; Gonzalez, B.
Title Novel polymerase chain reaction primers for the specific detection of bacterial copper P-type ATPases gene sequences in environmental isolates and metagenomic DNA Type
Year 2010 Publication Letters In Applied Microbiology Abbreviated Journal Lett. Appl. Microbiol.
Volume 50 Issue 6 Pages 552-562
Keywords ATPases; bacterial communities; copper resistance; metagenomic DNA; PCR primer pair
Abstract Aims: In the last decades, the worldwide increase in copper wastes release by industrial activities like mining has driven environmental metal contents to toxic levels. For this reason, the study of the biological copper-resistance mechanisms in natural environments is important. Therefore, an appropriate molecular tool for the detection and tracking of copper-resistance genes was developed. Methods and Results: In this work, we designed a PCR primer pair to specifically detect copper P-type ATPases gene sequences. These PCR primers were tested in bacterial isolates and metagenomic DNA from intertidal marine environments impacted by copper pollution. As well, T-RFLP fingerprinting of these gene sequences was used to compare the genetic composition of such genes in microbial communities, in normal and copper-polluted coastal environments. New copper P-type ATPases gene sequences were found, and a high degree of change in the genetic composition because of copper exposure was also determined. Conclusions: This PCR based method is useful to track bacterial copper-resistance gene sequences in the environment. Significance and Impact of the Study: This study is the first to report the design and use of a PCR primer pair as a molecular marker to track bacterial copper-resistance determinants, providing an excellent tool for long-term analysis of environmental communities exposed to metal pollution.
Address [Gonzalez, B.] Univ Adolfo Ibanez, Fac Ingn & Ciencia, Santiago 7941169, Chile, Email: bernardo.gonzalez@uai.cl
Corporate Author Thesis
Publisher Wiley-Blackwell Publishing, Inc Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume (down) Series Issue Edition
ISSN 0266-8254 ISBN Medium
Area Expedition Conference
Notes WOS:000277417000002 Approved
Call Number UAI @ eduardo.moreno @ Serial 88
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Author De la Iglesia, R.; Valenzuela-Heredia, D.; Andrade, S.; Correa, J.; Gonzalez, B.
Title Composition dynamics of epilithic intertidal bacterial communities exposed to high copper levels Type
Year 2012 Publication Fems Microbiology Ecology Abbreviated Journal FEMS Microbiol. Ecol.
Volume 79 Issue 3 Pages 720-727
Keywords copA; copper; epilithic bacteria; intertidal rocky shore; T-RFLP
Abstract Copper has a dual role for organisms, both as micronutrient and toxic element. Copper mining activities have an enormous ecological impact because of the extraction process and the consequent release of copper-containing waste materials to the environment. In northern Chile, mainly in the Chanaral coastal area, this phenomenon is clearly evident. The released waste material has caused a strong modification of the area, and copper enrichment of beaches and rocky shores has provoked a decrease in the richness and diversity of many species of macroorganisms. However, the effects that copper enrichment has on microbial (e.g. bacterial epilithic) communities associated with the rocky shore environment are poorly understood. Using a culture-independent molecular approach, field sampling and laboratory microcosm experiments, we determined the effects of copper enrichment on bacterial communities inhabiting the rocky shore environment. Field samples showed a strong effect of copper on the structure of the natural bacterial epilithic communities, and microcosm experiments demonstrated rapid changes in bacterial community when copper is added, and reversibility of this effect within 48 h after copper is removed.
Address [Gonzalez, Bernardo] Univ Adolfo Ibanez, Fac Ingn & Ciencias, Santiago 7941169, Chile, Email: bernardo.gonzalez@uai.cl
Corporate Author Thesis
Publisher Wiley-Blackwell Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume (down) Series Issue Edition
ISSN 0168-6496 ISBN Medium
Area Expedition Conference
Notes WOS:000299257300014 Approved
Call Number UAI @ eduardo.moreno @ Serial 192
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Author Plominsky, A.M.; Henriquez-Castillo, C.; Delherbe, N.; Podell, S.; Ramirez-Flandes, S.; Ugalde, J.A.; Santibanez, J.F.; van den Engh, G.; Hanselmann, K.; Ulloa, O.; De la Iglesia, R.; Allen, E.E.; Trefault, N.
Title Distinctive Archaeal Composition of an Artisanal Crystallizer Pond and Functional Insights Into Salt-Saturated Hypersaline Environment Adaptation Type
Year 2018 Publication Frontiers In Microbiology Abbreviated Journal Front. Microbiol.
Volume 9 Issue Pages 13 pp
Keywords hypersaline environments; solar salterns; metagenomics; microbial ecology; environmental adaptation; functional metagenomics; artisanal crystallizer pond
Abstract Hypersaline environments represent some of the most challenging settings for life on Earth. Extremely halophilic microorganisms have been selected to colonize and thrive in these extreme environments by virtue of a broad spectrum of adaptations to counter high salinity and osmotic stress. Although there is substantial data on microbial taxonomic diversity in these challenging ecosystems and their primary osmoadaptation mechanisms, less is known about how hypersaline environments shape the genomes of microbial inhabitants at the functional level. In this study, we analyzed the microbial communities in five ponds along the discontinuous salinity gradient from brackish to salt-saturated environments and sequenced the metagenome of the salt (halite) precipitation pond in the artisanal Cahuil Solar Saltern system. We combined field measurements with spectrophotometric pigment analysis and flow cytometry to characterize the microbial ecology of the pond ecosystems, including primary producers and applied metagenomic sequencing for analysis of archaeal and bacterial taxonomic diversity of the salt crystallizer harvest pond. Comparative metagenomic analysis of the Cahuil salt crystallizer pond against microbial communities from other salt-saturated aquatic environments revealed a dominance of the archaeal genus Halorubrum and showed an unexpectedly low abundance of Haloquadratum in the Cahuil system. Functional comparison of 26 hypersaline microbial metagenomes revealed a high proportion of sequences associated with nucleotide excision repair, helicases, replication and restriction-methylation systems in all of them. Moreover, we found distinctive functional signatures between the microbial communities from salt-saturated (>30% [w/v] total salinity) compared to sub-saturated hypersaline environments mainly due to a higher representation of sequences related to replication, recombination and DNA repair in the former. The current study expands our understanding of the diversity and distribution of halophilic microbial populations inhabiting salt-saturated habitats and the functional attributes that sustain them.
Address [Plominsky, Alvaro M.; Henriquez-Castillo, Carlos; Santibanez, Juan F.; Ulloa, Osvaldo] Univ Concepcion, Fac Nat & Oceanog Sci, Dept Oceanog, Concepcion, Chile, Email: eallen@ucsd.edu;
Corporate Author Thesis
Publisher Frontiers Media Sa Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume (down) Series Issue Edition
ISSN 1664-302x ISBN Medium
Area Expedition Conference
Notes WOS:000441537100001 Approved
Call Number UAI @ eduardo.moreno @ Serial 895
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