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Author (up) Espinoza, C.; Seccatore, J.; Herrera, M.
Title Chilean artisanal mining: a gambling scenario Type
Year 2020 Publication REM – International Engineering Journal Abbreviated Journal REM
Volume 73 Issue 2 Pages
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Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 2448-167X ISBN Medium
Area Expedition Conference
Notes Approved
Call Number UAI @ eduardo.moreno @ Serial 1311
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Author (up) Romero-Romero, J.L.; Inostroza-Blancheteau, C.; Reyes-D?az, M.; Matte, J.P.; Aquea, F.; Espinoza, C.; Gil, P.M.; Arce-johnson, P.
Title Increased Drought and Salinity Tolerance in Citrus aurantifolia (Mexican Lemon) Plants Overexpressing Arabidopsis CBF3 Gene Type
Year 2020 Publication Journal Of Soil Science And Plant Nutrition Abbreviated Journal J. Soil Sci. Plant Nutr.
Volume 20 Issue 1 Pages 244-252
Keywords Drought tolerance; Abiotic stress; Transgenics citrus; CBF3 transcription factor
Abstract Citrus are a globally important fruit crop. Abiotic stressors such as drought and salinity adversely affect physiological citrus performance and survival. With the aim of improving drought tolerance in citrus plants, we constructed transgenic lines of Citrus lemon overexpressing the Arabidopsis transcription factor CBF3. Molecular, physiological, and quantitative real-time analyses showed high expression of AtCBF3 in three selected transgenic lines. During a 15-day treatment of water deficit by cessation of irrigation, the transgenic lines LM2 and LM14 showed lower stomatal conductance and transpiration paired with lower photosynthesis, whereas transgenic line LM7 maintained its photosynthesis, declining stomatal conductance, and transpiration compared to WT plants, which is manifested into more efficient water use. The genes CsRafS1 and CsGolS1 showed similar or greater expression in one of the transgenic lines with respect to control plants. Moreover, transgenic lines were more tolerant to saline stress and presented a greener phenotype with increased chlorophyll content in leaf discs compared to WT plants. In addition, a lower electrical conductivity in solution was observed in transgenic lines. Furthermore, all transgenic lines exhibited significantly less accumulation of reactive oxygen species than WT plants. Together, these results suggest the potential for heterologous expression of the AtCBF3 gene to mediate tolerance to hydric and saline stress in citrus plants.
Address [Romero-Romero, Jesus L.] CIIIDIR, Inst Politecn Nacl, Dept Biotecnol Agr, Unidad Sinaloa, Guasave, Sinaloa, Mexico, Email: parce@bio.puc.cl
Corporate Author Thesis
Publisher Springer International Publishing Ag Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0718-9508 ISBN Medium
Area Expedition Conference
Notes WOS:000520138000021 Approved
Call Number UAI @ eduardo.moreno @ Serial 1158
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