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Author Selcho, M.; Millan, C.; Palacios-Munoz, A.; Ruf, F.; Ubillo, L.; Chen, J.T.; Bergmann, G.; Ito, C.; Silva, V.; Wegener, C.; Ewer, J. pdf  doi
openurl 
  Title Central and peripheral clocks are coupled by a neuropeptide pathway in Drosophila Type
  Year 2017 Publication Nature Communications Abbreviated Journal Nat. Commun.  
  Volume 8 Issue Pages 13 pp  
  Keywords  
  Abstract Animal circadian clocks consist of central and peripheral pacemakers, which are coordinated to produce daily rhythms in physiology and behaviour. Despite its importance for optimal performance and health, the mechanism of clock coordination is poorly understood. Here we dissect the pathway through which the circadian clock of Drosophila imposes daily rhythmicity to the pattern of adult emergence. Rhythmicity depends on the coupling between the brain clock and a peripheral clock in the prothoracic gland (PG), which produces the steroid hormone, ecdysone. Time information from the central clock is transmitted via the neuropeptide, sNPF, to non-clock neurons that produce the neuropeptide, PTTH. These secretory neurons then forward time information to the PG clock. We also show that the central clock exerts a dominant role on the peripheral clock. This use of two coupled clocks could serve as a paradigm to understand how daily steroid hormone rhythms are generated in animals.  
  Address [Selcho, Mareike; Ruf, Franziska; Chen, Jiangtian; Bergmann, Gregor; Ito, Chihiro; Wegener, Christian] Univ Wurzburg, Theodor Boveri Inst, Neurobiol & Genet, Bioctr, D-97074 Wurzburg, Germany, Email: christian.wegener@biozentrum.uni-wuerzburg.de;  
  Corporate Author Thesis  
  Publisher Nature Publishing Group Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 2041-1723 ISBN Medium  
  Area Expedition Conference  
  Notes WOS:000402306200001 Approved  
  Call Number UAI @ eduardo.moreno @ Serial 736  
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Author Sundram, V.; Ng, F.S.; Roberts, M.A.; Millan, C.; Ewer, J.; Jackson, F.R. pdf  doi
openurl 
  Title Cellular Requirements for LARK in the Drosophila Circadian System Type
  Year 2012 Publication Journal Of Biological Rhythms Abbreviated Journal J. Biol. Rhythms  
  Volume 27 Issue 3 Pages 183-195  
  Keywords clock output; posttranscriptional; RNA binding; locomotor activity; eclosion  
  Abstract RNA-binding proteins mediate posttranscriptional functions in the circadian systems of multiple species. A conserved RNA recognition motif (RRM) protein encoded by the lark gene is postulated to serve circadian output and molecular oscillator functions in Drosophila and mammals, respectively. In no species, however, has LARK been eliminated, in vivo, to determine the consequences for circadian timing. The present study utilized RNA interference (RNAi) techniques in Drosophila to decrease LARK levels in clock neurons and other cell types in order to evaluate the circadian functions of the protein. Knockdown of LARK in timeless (TIM)- or pigment dispersing factor (PDF)-containing clock cells caused a significant number of flies to exhibit arrhythmic locomotor activity, demonstrating a requirement for the protein in pacemaker cells. There was no obvious effect on PER protein cycling in lark interference (RNAi) flies, but a knockdown within the PDF neurons was associated with increased PDF immunoreactivity at the dorsal termini of the small ventral lateral neuronal (s-LNv) projections, suggesting an effect on neuropeptide release. The expression of lark RNAi in multiple neurosecretory cell populations demonstrated that LARK is required within pacemaker and nonpacemaker cells for the manifestation of normal locomotor activity rhythms. Interestingly, decreased LARK function in the prothoracic gland (PG), a peripheral organ containing a clock required for the circadian control of eclosion, was associated with weak population eclosion rhythms or arrhythmicity.  
  Address [Sundram, Vasudha; Ng, Fanny S.; Roberts, Mary A.; Jackson, F. Rob] Tufts Univ, Sch Med, Dept Neurosci, Ctr Neurosci Res, Boston, MA 02111 USA, Email: rob.jackson@tufts.edu  
  Corporate Author Thesis  
  Publisher Sage Publications Inc Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0748-7304 ISBN Medium  
  Area Expedition Conference  
  Notes WOS:000304715700001 Approved  
  Call Number UAI @ eduardo.moreno @ Serial 219  
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