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Perez-Pantoja, D.; Leiva-Novoa, P.; Donoso, R.A.; Little, C.; Godoy, M.; Pieper, D.H.; Gonzalez, B. |
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Title  |
Hierarchy of Carbon Source Utilization in Soil Bacteria: Hegemonic Preference for Benzoate in Complex Aromatic Compound Mixtures Degraded by Cupriavidus pinatubonensis Strain JMP134 |
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Year |
2015 |
Publication |
Applied And Environmental Microbiology |
Abbreviated Journal |
Appl. Environ. Microbiol. |
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Volume |
81 |
Issue |
12 |
Pages |
3914-3924 |
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Abstract |
Cupriavidus pinatubonensis JMP134, like many other environmental bacteria, uses a range of aromatic compounds as carbon sources. Previous reports have shown a preference for benzoate when this bacterium grows on binary mixtures composed of this aromatic compound and 4-hydroxybenzoate or phenol. However, this observation has not been extended to other aromatic mixtures resembling a more archetypal context. We carried out a systematic study on the substrate preference of C. pinatubonensis JMP134 growing on representative aromatic compounds channeled through different catabolic pathways described in aerobic bacteria. Growth tests of nearly the entire set of binary combinations and in mixtures composed of 5 or 6 aromatic components showed that benzoate and phenol were always the preferred and deferred growth substrates, respectively. This pattern was supported by kinetic analyses that showed shorter times to initiate consumption of benzoate in aromatic compound mixtures. Gene expression analysis by real-time reverse transcription-PCR (RT-PCR) showed that, in all mixtures, the repression by benzoate over other catabolic pathways was exerted mainly at the transcriptional level. Additionally, inhibition of benzoate catabolism suggests that its multiple repressive actions are not mediated by a sole mechanism, as suggested by dissimilar requirements of benzoate degradation for effective repression in different aromatic compound mixtures. The hegemonic preference for benzoate over multiple aromatic carbon sources is not explained on the basis of growth rate and/or biomass yield on each single substrate or by obvious chemical or metabolic properties of these aromatic compounds. |
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Address |
[Leiva-Novoa, Pablo; Donoso, Raul A.; Little, Cedric; Gonzalez, Bernardo] Univ Adolfo Ibanez, Ctr Appl Ecol & Sustainabil, Fac Ingn & Ciencias, Santiago, Chile, Email: bernardo.gonzalez@uai.cl |
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Amer Soc Microbiology |
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English |
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ISSN |
0099-2240 |
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WOS:000354864000002 |
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UAI @ eduardo.moreno @ |
Serial |
491 |
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Author |
Trefault, N.; Guzman, L.; Perez, H.; Godoy, M.; Gonzalez, B. |
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Title |
Involvement of several transcriptional regulators in the differential expression of tfd genes in Cupriavidus necator JMP134 |
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Year |
2009 |
Publication |
International Microbiology |
Abbreviated Journal |
Int. Microbiol. |
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Volume |
12 |
Issue |
2 |
Pages |
97-106 |
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Keywords |
Cupriavidus necator; LysR transcriptional regulators; pJP4 catabolic plasmid; tfd catabolic genes |
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Abstract |
Cupriavidus necator JMP134 has been extensively studied because of its ability to degrade chloroaromatic compounds, including the herbicides 2,4-dichlorophenoxyacetic acid (2,4-D) and 3-chlorobenzoic acid (3-CB), which is achieved through the pJP4-encoded chlorocatechol degradation gene clusters: tfdC(I)D(I)E(I)F(I), and tfdD(II)C(II)E(II)F(II). The present work describes a different tfd-genes expression profile depending on whether C. necator cells were induced with 2,4-D or 3-CB. By contrast, in vitro binding assays of the Purified transcriptional activator TfdR showed similar binding to both tfd intergenic regions; these results were confirmed by in Vivo Studies of the expression of transcriptional lacZ fusions for these intergenic regions. Experiments aimed at investigating whether other pJP4 plasmid or chromosomal regulatory proteins could contribute to the differences in the response of both tfd promoters to induction by 2,4-D and 3-CB showed that the transcriptional regulators from the benzoate degradation pathway, CatR I and CatR2, affected 3-CB- and 2,4-D-related growth capabilities. It was also determined that the ISJP4-interrupted protein TfdT decreased growth on 3-CB. In addition, an ORF with 34% amino acid identity to IcIR-type transcriptional regulator members and located near the tfd(II) gene cluster module was shown to modulate the 2,4-D growth capability. Taken together, these results Suggest that tfd transcriptional regulation in C. necator JMP134 is far more complex than previously thought and that it involves proteins from different transcriptional regulator families. [Int Microbiol 2009; 12(2):97-106] |
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Address |
[Gonzalez, Bernardo] Univ Adolfo Ibanez, Fac Ingn & Ciencia, Santiago 7941169, Chile, Email: bernardo.gonzalez@uai.cl |
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Viguera Editores, S L |
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English |
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1139-6709 |
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WOS:000269753900003 |
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UAI @ eduardo.moreno @ |
Serial |
57 |
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