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Author Aquea, F.; Timmermann, T.; Herrera-Vasquez, A. pdf  doi
  Title Chemical inhibition of the histone acetyltransferase activity in Arabidopsis thaliana Type
  Year 2017 Publication Biochemical And Biophysical Research Communications Abbreviated Journal Biochem. Biophys. Res. Commun.  
  Volume (down) 483 Issue 1 Pages 664-668  
  Keywords Histone acetyltransferase; GCN5; Chemical inhibitor; Chromatin remodeling; Arabidopsis  
  Abstract Chemical inhibition of chromatin regulators provides an effective approach to investigate the roles of chromatin modifications in plant and animals. In this work, chemical inhibition of the Arabidopsis histone acetyltransferase activity by gamma-butyrolactone (MB-3), the inhibitor of the catalytic activity of mammalian GENERAL CONTROL NON-REPRESSIBLE 5 (GCN5) is evaluated. Arabidopsis seedlings were germinated in LS medium supplemented with different concentrations of MB-3, and inhibition in the root length and yellowed leaves were observed. The yellowed leaves phenotype of the plants grown in 100 μM of MB-3 was reverted when plants were additionally treated with 1 μM of TSA, a histone deacetylase inhibitor. Using an immunoblot assay with specific antibodies revealed a reduction of H3K14 acetylation levels at 3 and 24 h post-treatment. At 24 h post-treatment a reduction of H3K9 acetylation levels was observed. Targets of GCN5 related to stress were downregulated at 3 h post-treatment but no change was observed in target genes related to developmental transition. Our results indicate that MB-3 is a chemical inhibitor of the histone acetyltransferase in Arabidopsis and suggest that this inhibitor could function in other plants species. (C) 2016 Elsevier Inc. All rights reserved.  
  Address [Aquea, Felipe; Timmermann, Tania] Univ Adolfo Ibanez, Fac Ingn & Ciencias, Lab Bioingn, Santiago, Chile, Email:  
  Corporate Author Thesis  
  Publisher Academic Press Inc Elsevier Science Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0006-291x ISBN Medium  
  Area Expedition Conference  
  Notes WOS:000397259000103 Approved  
  Call Number UAI @ eduardo.moreno @ Serial 711  
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