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Author Romero-Romero, J.L.; Inostroza-Blancheteau, C.; Orellana, D.; Aquea, F.; Reyes-Diaz, M.; Gil, P.M.; Matte, J.P.; Arce-Johnson, P. pdf  doi
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  Title Stomata regulation by tissue-specific expression of the Citrus sinensis MYB61 transcription factor improves water-use efficiency in Arabidopsis Type
  Year 2018 Publication Plant Physiology And Biochemistry Abbreviated Journal Plant Physiol. Biochem.  
  Volume (down) 130 Issue Pages 54-60  
  Keywords Drought tolerance; Abiotic stress; Water use efficiency; Arabidopsis; Cisgenic; MYB61 transcription factor; Citrus  
  Abstract Water-use efficiency (WUE) is a quantitative measurement of biomass produced per volume of water transpired by a plant. WUE is an important physiological trait for drought response to mitigate the water deficiency. In this work, a cisgenic construction from Citrus sinensis was developed and its function in the improvement of WUE was evaluated in Arabidopsis. Sequences of the CsMYB61 coding region, a transcription factor implicated in the closure of stomata, together with a putative stomata-specific promoter from CsMYB.1.5, were identified and cloned. The protein encoded in the CsMYB61 locus harbors domains and motifs characteristic of MYB61 proteins. In addition, a 1.2 kb promoter region of the gene CsMYB15 (pCsMYB15) containing regulatory elements for expression in guard cells and in response to Abscisic Acid (ABA) and light was isolated. In Arabidopsis, pCsMYB15 directs the expression of the reporter gene GUS in stomata in the presence of light. In addition, transgenic lines expressing the CsMYB61 coding region under transcriptional control of pCsMYB15 have a normal phenotype under in vitro and greenhouse conditions. These transgenic lines exhibited a smaller opening of the stomata pore, lower stomatal conductance and respiration rate, enhanced sensitivity to exogenous ABA, and high drought stress tolerance. Our results indicate that stomata-specific expression of CsMYB61 enhances water use efficiency under drought conditions in Arabidospis.  
  Address [Romero-Romero, Jesus L.; Orellana, Daniela; Pablo Matte, Juan; Arce-Johnson, Patricio] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Genet Mol & Microbiol, Santiago, Chile, Email: parce@bio.puc.cl  
  Corporate Author Thesis  
  Publisher Elsevier France-Editions Scientifiques Medicales Elsevier Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0981-9428 ISBN Medium  
  Area Expedition Conference  
  Notes WOS:000444789200006 Approved  
  Call Number UAI @ eduardo.moreno @ Serial 915  
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Author Romero-Romero, J.L.; Inostroza-Blancheteau, C.; Reyes-D?az, M.; Matte, J.P.; Aquea, F.; Espinoza, C.; Gil, P.M.; Arce-johnson, P. doi  openurl
  Title Increased Drought and Salinity Tolerance in Citrus aurantifolia (Mexican Lemon) Plants Overexpressing Arabidopsis CBF3 Gene Type
  Year 2020 Publication Journal Of Soil Science And Plant Nutrition Abbreviated Journal J. Soil Sci. Plant Nutr.  
  Volume (down) 20 Issue 1 Pages 244-252  
  Keywords Drought tolerance; Abiotic stress; Transgenics citrus; CBF3 transcription factor  
  Abstract Citrus are a globally important fruit crop. Abiotic stressors such as drought and salinity adversely affect physiological citrus performance and survival. With the aim of improving drought tolerance in citrus plants, we constructed transgenic lines of Citrus lemon overexpressing the Arabidopsis transcription factor CBF3. Molecular, physiological, and quantitative real-time analyses showed high expression of AtCBF3 in three selected transgenic lines. During a 15-day treatment of water deficit by cessation of irrigation, the transgenic lines LM2 and LM14 showed lower stomatal conductance and transpiration paired with lower photosynthesis, whereas transgenic line LM7 maintained its photosynthesis, declining stomatal conductance, and transpiration compared to WT plants, which is manifested into more efficient water use. The genes CsRafS1 and CsGolS1 showed similar or greater expression in one of the transgenic lines with respect to control plants. Moreover, transgenic lines were more tolerant to saline stress and presented a greener phenotype with increased chlorophyll content in leaf discs compared to WT plants. In addition, a lower electrical conductivity in solution was observed in transgenic lines. Furthermore, all transgenic lines exhibited significantly less accumulation of reactive oxygen species than WT plants. Together, these results suggest the potential for heterologous expression of the AtCBF3 gene to mediate tolerance to hydric and saline stress in citrus plants.  
  Address [Romero-Romero, Jesus L.] CIIIDIR, Inst Politecn Nacl, Dept Biotecnol Agr, Unidad Sinaloa, Guasave, Sinaloa, Mexico, Email: parce@bio.puc.cl  
  Corporate Author Thesis  
  Publisher Springer International Publishing Ag Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0718-9508 ISBN Medium  
  Area Expedition Conference  
  Notes WOS:000520138000021 Approved  
  Call Number UAI @ eduardo.moreno @ Serial 1158  
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