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Author (up) Dauelsberg, P.; Matus, J.T.; Poupin, M.J.; Leiva-Ampuero, A.; Godoy, F.; Vega, A.; Arce-Johnson, P.
Title Effect of pollination and fertilization on the expression of genes related to floral transition, hormone synthesis and berry development in grapevine Type
Year 2011 Publication Journal Of Plant Physiology Abbreviated Journal J. Plant Physiol.
Volume 168 Issue 14 Pages 1667-1674
Keywords Anthesis; ASB1; Berry flesh; Embryo development; Flower patterning; L1L; Parthenocarpy
Abstract In the present work, the effect of assisted fertilization on anatomical, morphological and gene expression changes occurring in carpels and during early stages of berry development in Vitis vinifera were studied. Inflorescences were emasculated before capfall, immediately manually pollinated (EP) and fruit development was compared to emasculated but non-pollinated (ENP) and self-pollinated inflorescences (NESP). The diameter of berries derived from pollinated flowers (EP and NESP) was significantly higher than from non-pollinated flowers (ENP) at 21 days after emasculation/pollination (DAE), and a rapid increase in the size of the inner mesocarp, together with the presence of an embryo-like structure, were observed. The expression of gibberellin oxidases (GA200x and GA2ox), anthranilate synthase (related to auxin synthesis) and cytokinin synthase coding genes was studied to assess the relationship between hormone synthesis and early berry development, while flower patterning genes were analyzed to describe floral transition. Significant expression changes were found for hormone-related genes, suggesting that their expression at early stages of berry development (13 DAE) is related to cell division and differentiation of mesocarp tissue at a later stage (21 DAE). Expression of hormone-related genes also correlates with the expression of VvHB13, a gene related to mesocarp expansion, and with an increased repression of floral patterning genes (PISTILLATA and TM6), which may contribute to prevent floral transition inhibiting fruit growth before fertilization takes place. (C) 2011 Elsevier GmbH. All rights reserved.
Address [Dauelsberg, P; Matus, JT; Leiva-Ampuero, A; Godoy, F; Vega, A; Arce-Johnson, P] Pontificia Univ Catolica Chile, Dept Mol Genet & Microbiol, Santiago, Chile, Email: parce@bio.puc.cl
Corporate Author Thesis
Publisher Elsevier Gmbh, Urban & Fischer Verlag Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0176-1617 ISBN Medium
Area Expedition Conference
Notes WOS:000294832300008 Approved
Call Number UAI @ eduardo.moreno @ Serial 161
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Author (up) Loyola, R.; Herrera, D.; Mas, A.; Wong, D.C.J.; Holl, J.; Cavallini, E.; Amato, A.; Azuma, A.; Ziegler, T.; Aquea, F.; Castellarin, S.D.; Bogs, J.; Tornielli, G.B.; Pena-Neira, A.; Czemmel, S.; Alcalde, J.A.; Matus, J.T.; Arce-Johnson, P.
Title The photomorphogenic factors UV-B RECEPTOR 1, ELONGATED HYPOCOTYL 5, and HY5 HOMOLOGUE are part of the UV-B signalling pathway in grapevine and mediate flavonol accumulation in response to the environment Type
Year 2016 Publication Journal Of Experimental Botany Abbreviated Journal J. Exp. Bot.
Volume 67 Issue 18 Pages 5429-5445
Keywords Binding; glycosyltransferase; MYBF1; network; photolyase; ripening; UVR8
Abstract By performing molecular studies coupled to radiation experiments and in silico systems analyses, we have ascertained the role of the grapevine UV-B receptor and two HY5 homologues in regulating flavonol synthesis.Grapevine (Vitis vinifera L.) is a species well known for its adaptation to radiation. However, photomorphogenic factors related to UV-B responses have not been molecularly characterized. We cloned and studied the role of UV-B RECEPTOR (UVR1), ELONGATED HYPOCOTYL 5 (HY5), and HY5 HOMOLOGUE (HYH) from V. vinifera. We performed gene functional characterizations, generated co-expression networks, and tested them in different environmental conditions. These genes complemented the Arabidopsis uvr8 and hy5 mutants in morphological and secondary metabolic responses to radiation. We combined microarray and RNA sequencing (RNA-seq) data with promoter inspections to identify HY5 and HYH putative target genes and their DNA binding preferences. Despite sharing a large set of common co-expressed genes, we found different hierarchies for HY5 and HYH depending on the organ and stress condition, reflecting both co-operative and partially redundant roles. New candidate UV-B gene markers were supported by the presence of HY5-binding sites. These included a set of flavonol-related genes that were up-regulated in a HY5 transient expression assay. We irradiated in vitro plantlets and fruits from old potted vines with high and low UV-B exposures and followed the accumulation of flavonols and changes in gene expression in comparison with non-irradiated conditions. UVR1, HY5, and HYH expression varied with organ, developmental stage, and type of radiation. Surprisingly, UVR1 expression was modulated by shading and temperature in berries, but not by UV-B radiation. We propose that the UV-B response machinery favours berry flavonol accumulation through the activation of HY5 and HYH at different developmental stages at both high and low UV-B exposures.
Address [Loyola, Rodrigo; Herrera, Daniela; Arce-Johnson, Patricio] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Genet Mol & Microbiol, Alameda 340, Santiago, Chile, Email: jalcalde@uc.cl;
Corporate Author Thesis
Publisher Oxford Univ Press Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0022-0957 ISBN Medium
Area Expedition Conference
Notes WOS:000386067000014 Approved
Call Number UAI @ eduardo.moreno @ Serial 667
Permanent link to this record