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Author (up) Uranga, R.M.; Millan, C.; Barahona, M.J.; Recabal, A.; Salgado, M.; Martinez, F.; Ordenes, P.; Elizondo-Vega, R.; Sepluveda, F.; Uribe, E.; Garcia-Robles, M.D.
Title Adenovirus-mediated suppression of hypothalamic glucokinase affects feeding behavior Type
Year 2017 Publication Scientific Reports Abbreviated Journal Sci Rep
Volume 7 Issue Pages 13 pp
Abstract Glucokinase (GK), the hexokinase involved in glucosensing in pancreatic beta-cells, is also expressed in arcuate nucleus (AN) neurons and hypothalamic tanycytes, the cells that surround the basal third ventricle (3V). Several lines of evidence suggest that tanycytes may be involved in the regulation of energy homeostasis. Tanycytes have extended cell processes that contact the feeding-regulating neurons in the AN, particularly, agouti-related protein (AgRP), neuropeptide Y (NPY), cocaine-and amphetamine-regulated transcript (CART) and proopiomelanocortin (POMC) neurons. In this study, we developed an adenovirus expressing GK shRNA to inhibit GK expression in vivo. When injected into the 3V of rats, this adenovirus preferentially transduced tanycytes. qRT-PCR and Western blot assays confirmed GK mRNA and protein levels were lower in GK knockdown animals compared to the controls. In response to an intracerebroventricular glucose injection, the mRNA levels of anorexigenic POMC and CART and orexigenic AgRP and NPY neuropeptides were altered in GK knockdown animals. Similarly, food intake, meal duration, frequency of eating events and the cumulative eating time were increased, whereas the intervals between meals were decreased in GK knockdown rats, suggesting a decrease in satiety. Thus, GK expression in the ventricular cells appears to play an important role in feeding behavior.
Address [Maria Uranga, Romina; Millan, Carola; Jose Barahona, Maria; Recabal, Antonia; Salgado, Magdiel; Martinez, Fernando; Ordenes, Patricio; Elizondo-Vega, Roberto; Sepluveda, Fernando; de los Angeles Garcia-Robles, Maria] Univ Concepcion, Dept Biol Celular, Concepcion, Chile, Email: mgarcia@udec.cl
Corporate Author Thesis
Publisher Nature Publishing Group Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 2045-2322 ISBN Medium
Area Expedition Conference
Notes WOS:000403413700059 Approved
Call Number UAI @ eduardo.moreno @ Serial 740
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