|   | 
Author (up) de la Guardia, ARH.; Ugalde, MB.; Lobos-Diaz, V.; Romero-Romero, JL.; Meyer-Regueiro, C.; Inostroza-Blancheteau, C.; Reyes-Diaz, M.; Aquea, F.; Arce-Johnson, P.
Title Isolation and molecular characterization of MYB60 in Solanum lycopersicum Type
Year 2021 Publication Molecular Biology Reports Abbreviated Journal Mol. Biol. Rep.
Volume 48 Issue 2 Pages 1579-1587
Keywords Abiotic stress; Drought; MYB60; Tomato; Stomatal aperture
Abstract Stomatal closure is a common adaptation response of plants to the onset of drought condition and its regulation is controlled by transcription factors. MYB60, a transcription factor involved in the regulation of light-induced stomatal opening, has been characterized in arabidopsis and grapevine. In this work, we studied the role of MYB60 homolog SIMYB60 in tomato plants. We identified, isolated, and sequenced the SIMYB60 coding sequence, and found domains and motifs characteristic of other MYB60 proteins. We determined that SlMYB60 is mainly expressed in leaves, and its expression is repressed by abscisic acid. Next, we isolated a putative promoter region containing regulatory elements responsible for guard cell expression and other putative regulatory elements related to ABA repression and vascular tissue expression. Protein localization assays demonstrated that SlMYB60 localizes to the nucleus. Finally, SlMYB60 is able to complement the mutant phenotype of atmyb60-1 in Arabidopsis. Together, these results indicate that SlMYB60 is the homologous gene in tomato and potentially offer a molecular target to improve crops.
Corporate Author Thesis
Publisher Place of Publication Editor
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0301-4851 ISBN Medium
Area Expedition Conference
Notes WOS:000612378400001 Approved
Call Number UAI @ alexi.delcanto @ Serial 1332
Permanent link to this record

Author (up) Romero-Romero, J.L.; Inostroza-Blancheteau, C.; Orellana, D.; Aquea, F.; Reyes-Diaz, M.; Gil, P.M.; Matte, J.P.; Arce-Johnson, P.
Title Stomata regulation by tissue-specific expression of the Citrus sinensis MYB61 transcription factor improves water-use efficiency in Arabidopsis Type
Year 2018 Publication Plant Physiology And Biochemistry Abbreviated Journal Plant Physiol. Biochem.
Volume 130 Issue Pages 54-60
Keywords Drought tolerance; Abiotic stress; Water use efficiency; Arabidopsis; Cisgenic; MYB61 transcription factor; Citrus
Abstract Water-use efficiency (WUE) is a quantitative measurement of biomass produced per volume of water transpired by a plant. WUE is an important physiological trait for drought response to mitigate the water deficiency. In this work, a cisgenic construction from Citrus sinensis was developed and its function in the improvement of WUE was evaluated in Arabidopsis. Sequences of the CsMYB61 coding region, a transcription factor implicated in the closure of stomata, together with a putative stomata-specific promoter from CsMYB.1.5, were identified and cloned. The protein encoded in the CsMYB61 locus harbors domains and motifs characteristic of MYB61 proteins. In addition, a 1.2 kb promoter region of the gene CsMYB15 (pCsMYB15) containing regulatory elements for expression in guard cells and in response to Abscisic Acid (ABA) and light was isolated. In Arabidopsis, pCsMYB15 directs the expression of the reporter gene GUS in stomata in the presence of light. In addition, transgenic lines expressing the CsMYB61 coding region under transcriptional control of pCsMYB15 have a normal phenotype under in vitro and greenhouse conditions. These transgenic lines exhibited a smaller opening of the stomata pore, lower stomatal conductance and respiration rate, enhanced sensitivity to exogenous ABA, and high drought stress tolerance. Our results indicate that stomata-specific expression of CsMYB61 enhances water use efficiency under drought conditions in Arabidospis.
Address [Romero-Romero, Jesus L.; Orellana, Daniela; Pablo Matte, Juan; Arce-Johnson, Patricio] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Genet Mol & Microbiol, Santiago, Chile, Email: parce@bio.puc.cl
Corporate Author Thesis
Publisher Elsevier France-Editions Scientifiques Medicales Elsevier Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0981-9428 ISBN Medium
Area Expedition Conference
Notes WOS:000444789200006 Approved
Call Number UAI @ eduardo.moreno @ Serial 915
Permanent link to this record