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Author Luengwilai, K.; Yu, J.W.; Jimenez, R.C.; Thitisaksakul, M.; Vega, A.; Dong, S.Y.; Beckles, D.M.
Title (up) Ectopic Expression of Arabidopsis thaliana zDof1.3 in Tomato (Solanum lycopersicum L.) Is Associated with Improved Greenhouse Productivity and Enhanced Carbon and Nitrogen Use Type
Year 2022 Publication International Journal of Molecular Sciences Abbreviated Journal Int. J. Mol. Sci.
Volume 23 Issue 19 Pages 11229
Keywords Dof; transcription factor; tomato; carbohydrates; nitrogen metabolism
Abstract A large collection of transgenic tomato lines, each ectopically expressing a different Arabidopsis thaliana transcription factor, was screened for variants with alterations in leaf starch. Such lines may be affected in carbon partitioning, and in allocation to the sinks. We focused on 'L4080', which harbored an A. thaliana zDof (DNA-binding one zinc finger) isoform 1.3 (AtzDof1.3) gene, and which had a 2-4-fold higher starch-to-sucrose ratio in source leaves over the diel (p < 0.05). Our aim was to determine whether there were associated effects on productivity. L4080 plants were altered in nitrogen (N) and carbon (C) metabolism. The N-to-C ratio was higher in six-week-old L4080, and when treated with 1/10 N, L4080 growth was less inhibited compared to the wild-type and this was accompanied by faster root elongation (p < 0.05). The six-week-old L4080 acquired 42% more dry matter at 720 ppm CO2, compared to ambient CO2 (p < 0.05), while the wild-type (WT) remained unchanged. GC-MS-TOF data showed that L4080 source leaves were enriched in amino acids compared to the WT, and at 49 DPA, fruit had 25% greater mass, higher sucrose, and increased yield (25%; p < 0.05) compared to the WT. An Affymetrix cDNA array analysis suggested that only 0.39% of the 9000 cDNAs were altered by 1.5-fold (p < 0.01) in L4080 source leaves. C-14-labeling of fruit disks identified potential differences in 14-DPA fruit metabolism suggesting that post-transcriptional regulation was important. We conclude that AtzDof1.3 and the germplasm derived therefrom, should be investigated for their 'climate-change adaptive' potential.
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 1422-0067 ISBN Medium
Area Expedition Conference
Notes WOS:000867764100001 Approved
Call Number UAI @ alexi.delcanto @ Serial 1675
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Author Dauelsberg, P.; Matus, J.T.; Poupin, M.J.; Leiva-Ampuero, A.; Godoy, F.; Vega, A.; Arce-Johnson, P.
Title (up) Effect of pollination and fertilization on the expression of genes related to floral transition, hormone synthesis and berry development in grapevine Type
Year 2011 Publication Journal Of Plant Physiology Abbreviated Journal J. Plant Physiol.
Volume 168 Issue 14 Pages 1667-1674
Keywords Anthesis; ASB1; Berry flesh; Embryo development; Flower patterning; L1L; Parthenocarpy
Abstract In the present work, the effect of assisted fertilization on anatomical, morphological and gene expression changes occurring in carpels and during early stages of berry development in Vitis vinifera were studied. Inflorescences were emasculated before capfall, immediately manually pollinated (EP) and fruit development was compared to emasculated but non-pollinated (ENP) and self-pollinated inflorescences (NESP). The diameter of berries derived from pollinated flowers (EP and NESP) was significantly higher than from non-pollinated flowers (ENP) at 21 days after emasculation/pollination (DAE), and a rapid increase in the size of the inner mesocarp, together with the presence of an embryo-like structure, were observed. The expression of gibberellin oxidases (GA200x and GA2ox), anthranilate synthase (related to auxin synthesis) and cytokinin synthase coding genes was studied to assess the relationship between hormone synthesis and early berry development, while flower patterning genes were analyzed to describe floral transition. Significant expression changes were found for hormone-related genes, suggesting that their expression at early stages of berry development (13 DAE) is related to cell division and differentiation of mesocarp tissue at a later stage (21 DAE). Expression of hormone-related genes also correlates with the expression of VvHB13, a gene related to mesocarp expansion, and with an increased repression of floral patterning genes (PISTILLATA and TM6), which may contribute to prevent floral transition inhibiting fruit growth before fertilization takes place. (C) 2011 Elsevier GmbH. All rights reserved.
Address [Dauelsberg, P; Matus, JT; Leiva-Ampuero, A; Godoy, F; Vega, A; Arce-Johnson, P] Pontificia Univ Catolica Chile, Dept Mol Genet & Microbiol, Santiago, Chile, Email: parce@bio.puc.cl
Corporate Author Thesis
Publisher Elsevier Gmbh, Urban & Fischer Verlag Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0176-1617 ISBN Medium
Area Expedition Conference
Notes WOS:000294832300008 Approved
Call Number UAI @ eduardo.moreno @ Serial 161
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Author Poupin, M.J.; Timmermann, T.; Vega, A.; Zuniga, A.; Gonzalez, B.
Title (up) Effects of the Plant Growth-Promoting Bacterium Burkholderia phytofirmans PsJN throughout the Life Cycle of Arabidopsis thaliana Type
Year 2013 Publication Plos One Abbreviated Journal PLoS One
Volume 8 Issue 7 Pages 15 pp
Keywords
Abstract Plant growth-promoting rhizobacteria (PGPR) induce positive effects in plants, such as increased growth or reduced stress susceptibility. The mechanisms behind PGPR/plant interaction are poorly understood, as most studies have described short-term responses on plants and only a few studies have analyzed plant molecular responses under PGPR colonization. Here, we studied the effects of the PGPR bacterial model Burkholderia phytofirmans PsJN on the whole life cycle of Arabidopsis thaliana plants. We reported that at different plant developmental points, strain PsJN can be found in the rhizosphere and also colonizing their internal tissues. In early ontogeny, strain PsJN increased several growth parameters and accelerated growth rate of the plants. Also, an Arabidopsis transcriptome analysis revealed that 408 genes showed differential expression in PsJN-inoculated plants; some of these genes are involved in stress response and hormone pathways. Specifically, genes implicated in auxin and gibberellin pathways were induced. Quantitative transcriptional analyses of selected genes in different developmental stages revealed that the beginning of these changes could be evidenced early in development, especially among the down-regulated genes. The inoculation with heat-killed bacteria provoked a more severe transcriptional response in plants, but was not able to induce plant growth-promotion. Later in ontogeny, the growth rates of inoculated plants decreased with respect to the non-inoculated group and, interestingly, the inoculation accelerated the flowering time and the appearance of senescence signs in plants; these modifications correlate with the early up-regulation of flowering control genes. Then, we show that a single inoculation with a PGPR could affect the whole life cycle of a plant, accelerating its growth rate and shortening its vegetative period, both effects relevant for most crops. Thus, these findings provide novel and interesting aspects of these relevant biological interactions.
Address [Josefina Poupin, Maria; Timmermann, Tania; Zuniga, Ana; Gonzalez, Bernardo] Univ Adolfo Ibanez, Fac Ingn & Ciencias, Lab Bioingn, Santiago, Chile, Email: mpoupin@uai.cl
Corporate Author Thesis
Publisher Public Library Science Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 1932-6203 ISBN Medium
Area Expedition Conference
Notes WOS:000323110600043 Approved
Call Number UAI @ eduardo.moreno @ Serial 306
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Author Timmermann, T.; Poupin, M.J.; Vega, A.; Urrutia, C.; Ruz, G.A.; Gonzalez, B.
Title (up) Gene networks underlying the early regulation of Paraburkholderia phytofirmans PsJN induced systemic resistance in Arabidopsis Type
Year 2019 Publication Plos One Abbreviated Journal PLoS One
Volume 14 Issue 8 Pages 24 pp
Keywords
Abstract Plant defense responses to biotic stresses are complex biological processes, all governed by sophisticated molecular regulations. Induced systemic resistance (ISR) is one of these defense mechanisms where beneficial bacteria or fungi prime plants to resist pathogens or pest attacks. In ISR, the defense arsenal in plants remains dormant and it is only triggered by an infection, allowing a better allocation of plant resources. Our group recently described that the well-known beneficial bacterium Paraburkholderia phytofirmans PsJN is able to induce Arabidopsis thaliana resistance to Pseudomonas syringae pv. tomato (Pst) DC3000 through ISR, and that ethylene, jasmonate and salicylic acid are involved in this protection. Nevertheless, the molecular networks governing this beneficial interaction remain unknown. To tackle this issue, we analyzed the temporal changes in the transcriptome of PsJN-inoculated plants before and after being infected with Pst DC3000. These data were used to perform a gene network analysis to identify highly connected transcription factors. Before the pathogen challenge, the strain PsJN regulated 405 genes (corresponding to 1.8% of the analyzed genome). PsJN-inoculated plants presented a faster and stronger transcriptional response at 1-hour post infection (hpi) compared with the non-inoculated plants, which presented the highest transcriptional changes at 24 hpi. A principal component analysis showed that PsJN-induced plant responses to the pathogen could be differentiated from those induced by the pathogen itself. Forty-eight transcription factors were regulated by PsJN at 1 hpi, and a system biology analysis revealed a network with four clusters. Within these clusters LHY, WRKY28, MYB31 and RRTF1 are highly connected transcription factors, which could act as hub regulators in this interaction. Concordantly with our previous results, these clusters are related to jasmonate, ethylene, salicylic, acid and ROS pathways. These results indicate that a rapid and specific response of PsJN-inoculated plants to the virulent DC3000 strain could be the pivotal element in the protection mechanism.
Address [Timmermann, Tania; Josefina Poupin, Maria; Urrutia, Cristobal; Ruz, Gonzalo A.; Gonzalez, Bernardo] Univ Adolfo Ibanez, Lab Bioingn, Fac Ingn & Ciencias, Santiago, Chile, Email: bernardo.gonzalez@uai.cl
Corporate Author Thesis
Publisher Public Library Science Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 1932-6203 ISBN Medium
Area Expedition Conference
Notes WOS:000485036900060 Approved
Call Number UAI @ eduardo.moreno @ Serial 1054
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Author Aquea, F.; Vega, A.; Timmermann, T.; Poupin, M.J.; Arce-Johnson, P.
Title (up) Genome-wide analysis of the SET DOMAIN GROUP family in Grapevine Type
Year 2011 Publication Plant Cell Reports Abbreviated Journal Plant Cell Reports
Volume 30 Issue 6 Pages 1087-1097
Keywords Grapes; Vitis vinifera; Chromatin remodeling; Histone methyltransferase
Abstract The SET DOMAIN GROUP (SDG) proteins represent an evolutionarily-conserved family of epigenetic regulators present in eukaryotes and are putative candidates for the catalysis of lysine methylation in histones. Plant genomes analyses of this family have been performed in arabidopsis, maize, and rice and functional studies have shown that SDG genes are involved in the control of plant development. In this work, we describe the identification and structural characterization of SDG genes in the Vitis vinifera genome. This analysis revealed the presence of 33 putative SDG genes that can be grouped into different classes, as it has been previously described for plants. In addition to the SET domain, the proteins identified possessed other domains in the different classes. As part of our study regarding the growth and development of grapevine, we selected eight genes and their expression levels were analyzed in representative vegetative and reproductive organs of this species. The selected genes showed different patterns of expression during inflorescence and fruit development, suggesting that they participate in these processes. Furthermore, we showed that the expression of selected SDGs changes during viral infection, using as a model Grapevine Leafroll Associated Virus 3-infected symptomatic grapevine leaves and fruits. Our results suggest that developmental changes caused by this virus could be the result of alterations in SDG expression.
Address [Aquea, Felipe; Arce-Johnson, Patricio] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Genet Mol & Microbiol, Santiago, Chile, Email: parce@bio.puc.cl
Corporate Author Thesis
Publisher Springer Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0721-7714 ISBN Medium
Area Expedition Conference
Notes WOS:000290542800013 Approved
Call Number UAI @ eduardo.moreno @ Serial 184
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Author Perez-Lara, G.; Moyano, T.C.; Vega, A.; Larrondo, L.F.; Polanco, R.; Alvarez, J.M.; Aguayo, D.; Canessa, P.
Title (up) The Botrytis cinerea Gene Expression Browser Type
Year 2023 Publication Journal of Fungi Abbreviated Journal J. Fungi
Volume 9 Issue 1 Pages 84
Keywords Botrytis cinerea; RNA-Seq; phytopathogen; transcriptomics; Botrytis Expression Browser; BEB
Abstract For comprehensive gene expression analyses of the phytopathogenic fungus Botrytis cinerea, which infects a number of plant taxa and is a cause of substantial agricultural losses worldwide, we developed BEB, a web-based B. cinerea gene Expression Browser. This computationally inexpensive web-based application and its associated database contain manually curated RNA-Seq data for B. cinerea. BEB enables expression analyses of genes of interest under different culture conditions by providing publication-ready heatmaps depicting transcript levels, without requiring advanced computational skills. BEB also provides details of each experiment and user-defined gene expression clustering and visualization options. If needed, tables of gene expression values can be downloaded for further exploration, including, for instance, the determination of differentially expressed genes. The BEB implementation is based on open-source computational technologies that can be deployed for other organisms. In this case, the new implementation will be limited only by the number of transcriptomic experiments that are incorporated into the platform. To demonstrate the usability and value of BEB, we analyzed gene expression patterns across different conditions, with a focus on secondary metabolite gene clusters, chromosome-wide gene expression, previously described virulence factors, and reference genes, providing the first comprehensive expression overview of these groups of genes in this relevant fungal phytopathogen. We expect this tool to be broadly useful in B. cinerea research, providing a basis for comparative transcriptomics and candidate gene identification for functional assays.
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 2309-608X ISBN Medium
Area Expedition Conference
Notes WOS:000917555600001 Approved
Call Number UAI @ alexi.delcanto @ Serial 1756
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