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Author Baler, R.V.; Wijnhoven, I.B.; del Valle, V.I.; Giovanetti, C.M.; Vivanco, J.F.
Title Microporosity Clustering Assessment in Calcium Phosphate Bioceramic Particles Type
Year 2019 Publication Frontiers In Bioengineering And Biotechnology Abbreviated Journal Front. Bioeng. Biotechnol.
Volume 7 Issue 281 Pages 7 pp
Keywords calcium phosphate; bioceramic particle; microporosity; data mining; K-means clustering
Abstract There has been an increase in the application of different biomaterials to repair hard tissues. Within these biomaterials, calcium phosphate (CaP) bioceramics are suitable candidates, since they can be biocompatible, biodegradable, osteoinductive, and osteoconductive. Moreover, during sintering, bioceramic materials are prone to form micropores and undergo changes in their surface topographical features, which influence cellular physiology and bone ingrowth. In this study, five geometrical properties from the surface of CaP bioceramic particles and their micropores were analyzed by data mining techniques, driven by the research question: what are the geometrical properties of individual micropores in a CaP bioceramic, and how do they relate to each other? The analysis not only shows that it is feasible to determine the existence of micropore clusters, but also to quantify their geometrical properties. As a result, these CaP bioceramic particles present three groups of micropore clusters distinctive by their geometrical properties. Consequently, this new methodological clustering assessment can be applied to advance the knowledge about CaP bioceramics and their role in bone tissue engineering.
Address [Vallejos Baler, Raul; Irribarra del Valle, Victor; Vivanco, Juan F.] Adolfo Ibanez Univ, Fac Engn & Sci, Vina Del Mar, Chile, Email: juan.vivanco@uai.cl
Corporate Author Thesis
Publisher Frontiers Media Sa Place of Publication Editor
Language English Summary Language Original Title
Series Editor (up) Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 2296-4185 ISBN Medium
Area Expedition Conference
Notes WOS:000495370600001 Approved
Call Number UAI @ eduardo.moreno @ Serial 1062
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Author Wijnhoven, I.B.; Vallejos, R.; Santibanez, J.F.; Millan, C.; Vivanco, J.F.
Title Analysis of cell-biomaterial interaction through cellular bridge formation in the interface between hGMSCs and CaP bioceramics Type
Year 2020 Publication Scientific Reports Abbreviated Journal Sci Rep
Volume 10 Issue 1 Pages 14 pp
Keywords
Abstract The combination of biomaterials and stem cells for clinical applications constitute a great challenge in bone tissue engineering. Hence, cellular networks derived from cells-biomaterials crosstalk have a profound influence on cell behaviour and communication, preceding proliferation and differentiation. The purpose of this study was to investigate in vitro cellular networks derived from human gingival mesenchymal stem cells (hGMSCs) and calcium phosphate (CaP) bioceramic interaction. Biological performance of CaP bioceramic and hGMSCs interaction was evaluated through cell adhesion and distribution, cellular proliferation, and potential osteogenic differentiation, at three different times: 5 h, 1 week and 4 weeks. Results confirmed that hGMSCs met the required MSCs criteria while displaying osteogenic differentiaton capacities. We found a significant increase of cellular numbers and proliferation levels. Also, protein and mRNA OPN expression were upregulated in cells cultured with CaP bioceramic by day 21, suggesting an osteoinductible effect of the CaP bioceramic on hGMSCs. Remarkably, CaP bioceramic aggregations were obtained through hGMSCs bridges, suggesting the in vitro potential of macrostructures formation. We conclude that hGMSCs and CaP bioceramics with micro and macropores support hGMSC adhesion, proliferation and osteogenic differentiation. Our results suggest that investigations focused on the interface cells-biomaterials are essential for bone tissue regenerative therapies.
Address [Vallejos, Raul; Vivanco, Juan F.] Univ Adolfo Ibanez, Fac Ingn & Ciencias, Vina Del Mar, Chile, Email: juan.vivanco@uai.cl
Corporate Author Thesis
Publisher Nature Research Place of Publication Editor
Language English Summary Language Original Title
Series Editor (up) Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 2045-2322 ISBN Medium
Area Expedition Conference
Notes WOS:000577291800004 Approved
Call Number UAI @ alexi.delcanto @ Serial 1235
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