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Author Ledger, T.; Rojas, S.; Timmermann, T.; Pinedo, I.; Poupin, M.J.; Garrido, T.; Richter, P.; Tamayo, J.; Donoso, R.
Title Volatile-Mediated Effects Predominate in Paraburkholderia phytofirmans Growth Promotion and Salt Stress Tolerance of Arabidopsis thaliana Type
Year 2016 Publication Frontiers In Microbiology Abbreviated Journal Front. Microbiol.
Volume 7 Issue Pages 18 pp
Keywords plant growth promoting rhizobacteria (PGPR); Paraburkholderia phytofirmans PsJN; Arabidopsis thaliana; abiotic stress tolerance; ACC deaminase; volatile organic compounds (VOCs)
Abstract Abiotic stress has a growing impact on plant growth and agricultural activity worldwide. Specific plant growth promoting rhizobacteria have been reported to stimulate growth and tolerance to abiotic stress in plants, and molecular mechanisms like phytohormone synthesis and 1-aminocyclopropane-1-carboxylate deamination are usual candidates proposed to mediate these bacterial effects. Paraburkholderia phytofirmans PsJN is able to promote growth of several plant hosts, and improve their tolerance to chilling, drought and salinity. This work investigated bacterial determinants involved in PsJN stimulation of growth and salinity tolerance in Arabidopsis thaliana, showing bacteria enable plants to survive long-term salinity treatment, accumulating less sodium within leaf tissues relative to non-inoculated controls. Inactivation of specific bacterial genes encoding ACC deaminase, auxin catabolism, N-acyl-homosenne-lactone production, and flagellin synthesis showed these functions have little influence on bacterial induction of salinity tolerance. Volatile organic compound emission from strain PsJN was shown to reproduce the effects of direct bacterial inoculation of roots, increasing plant growth rate and tolerance to salinity evaluated both in vitro and in soil. Furthermore, early exposure to VOCs from P phytofirmans was sufficient to stimulate long-term effects observed in Arabidopsis growth in the presence and absence of salinity. Organic compounds were analyzed in the headspace of PsJN cultures, showing production of 2-undecanone, 7-hexanol, 3-methylbutanol and dimethyl disulfide. Exposure of A. thaliana to different quantities of these molecules showed that they are able to influence growth in a wide range of added amounts. Exposure to a blend of the first three compounds was found to mimic the effects of PsJN on both general growth promotion and salinity tolerance. To our knowledge, this is the first report on volatile compound-mediated induction of plant abiotic stress tolerance by a Paraburkholderia species.
Address [Ledger, Thomas; Rojas, Sandy; Timmermann, Tania; Pinedo, Ignacio; Poupin, Maria J.; Tamayo, Javier; Donoso, Raul] Univ Adolfo Ibanez, Fac Ingn & Ciencias, Lab Bioingn, Santiago, Chile, Email: tledger@uai.cl
Corporate Author Thesis
Publisher Frontiers Media Sa Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 1664-302x ISBN Medium
Area Expedition Conference
Notes WOS:000388754700001 Approved
Call Number UAI @ eduardo.moreno @ Serial 679
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Author Moraga, F.; Aquea, F.
Title Composition of the SAGA complex in plants and its role in controlling gene expression in response to abiotic stresses Type
Year 2015 Publication Frontiers In Plant Science Abbreviated Journal Front. Plant Sci.
Volume 6 Issue Pages 9 pp
Keywords SAGA complex; chromatin remodeling; transcriptional coactivator; abiotic stress; protein complex; histone acetyltransferase
Abstract Protein complexes involved in epigenetic regulation of transcription have evolved as molecular strategies to face environmental stress in plants. SAGA (Spt-Ada-Gcn5 Acetyltransferase) is a transcriptional co-activator complex that regulates numerous cellular processes through the coordination of multiple post-translational histone modifications, including acetylation, deubiquitination, and chromatin recognition. The diverse functions of the SAGA complex involve distinct modules that are highly conserved between yeast, flies, and mammals. In this review, the composition of the SAGA complex in plants is described and its role in gene expression regulation under stress conditions summarized. Some of these proteins are likely involved in the regulation of the inducible expression of genes under light, cold, drought, salt, and iron stress, although the functions of several of its components remain unknown.
Address [Moraga, Felipe; Aquea, Felipe] Univ Adolfo Ibanez, Fac Ingn & Ciencias, Lab Bioingn, Santiago, Chile, Email: felipe.aquea@uai.cl
Corporate Author Thesis
Publisher Frontiers Media Sa Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 1664-462x ISBN Medium
Area Expedition Conference
Notes WOS:000364240600001 Approved
Call Number UAI @ eduardo.moreno @ Serial 540
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Author Pinedo, I.; Ledger, T.; Greve, M.; Poupin, M.J.
Title Burkholderia phytofirmans PsJN induces long-term metabolic and transcriptional changes involved in Arabidopsis thaliana salt tolerance Type
Year 2015 Publication Frontiers In Plant Science Abbreviated Journal Front. Plant Sci.
Volume 6 Issue Pages 17 pp
Keywords plant growth promoting rhizobacteria (PGPR); ion transport; osmotic stress response; priming; abiotic stress tolerance; reactive oxygen species (ROS); rhizosphere; beneficial bacteria
Abstract Salinity is one of the major limitations for food production worldwide. Improvement of plant salt-stress tolerance using plant-growth promoting rhizobacteria (PGPR) has arisen as a promising strategy to help overcome this limitation. However, the molecular and biochemical mechanisms controlling PGPR/plant interactions under salt-stress remain unclear. The main objective of this study was to obtain new insights into the mechanisms underlying salt-stress tolerance enhancement in the salt-sensitive Arabidopsis thaliana Col-0 plants, when inoculated with the well-known PGPR strain Burkholderia phytofirmans PsJN. To tackle this, different life history traits, together with the spatiotemporal accumulation patterns for key metabolites and salt-stress related transcripts, were analyzed in inoculated plants under short and long-term salt-stress. Inoculated plants displayed faster recovery and increased tolerance after sustained salt-stress. PsJN treatment accelerated the accumulation of proline and transcription of genes related to abscisic acid signaling (Relative to Dessication, RD29A and RD29B), ROS scavenging Oscorbate Peroxidase 2), and detoxification (Glyoxalase I 7), and down-regulated the expression of Lipoxygenase 2 (related to jasmonic acid biosynthesis). Among the general transcriptional effects of this bacterium, the expression pattern of important ion-homeostasis related genes was altered after short and longterm stress (Arabidopsis K Transporter 1, High-Affinity K Transporter 1, Sodium Hydrogen Exchanger 2, and Arabidopsis Salt Overly Sensitive 1). In all, the faster and stronger molecular changes induced by the inoculation suggest a PsJN-priming effect, which may explain the observed tolerance after short-term and sustained salt-stress in plants. This study provides novel information about possible mechanisms involved in salt-stress tolerance induced by PGPR in plants, showing that certain changes are maintained over time. This opens up new venues to study these relevant biological associations, as well as new approaches to a better understanding of the spatiotemporal mechanisms involved in stress tolerance in plants.
Address [Pinedo, Ignacio; Ledger, Thomas; Greve, Macarena; Poupin, Maria J.] Univ Adolfo Ibanez, Fac Ingn & Ciencias, Lab Bioingn, Santiago 7941169, Chile, Email: mpoupin@uai.cl
Corporate Author Thesis
Publisher Frontiers Research Foundation Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 1664-462x ISBN Medium
Area Expedition Conference
Notes WOS:000357301200001 Approved
Call Number UAI @ eduardo.moreno @ Serial 509
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Author Romero-Romero, J.L.; Inostroza-Blancheteau, C.; Orellana, D.; Aquea, F.; Reyes-Diaz, M.; Gil, P.M.; Matte, J.P.; Arce-Johnson, P.
Title Stomata regulation by tissue-specific expression of the Citrus sinensis MYB61 transcription factor improves water-use efficiency in Arabidopsis Type
Year 2018 Publication Plant Physiology And Biochemistry Abbreviated Journal Plant Physiol. Biochem.
Volume 130 Issue Pages 54-60
Keywords Drought tolerance; Abiotic stress; Water use efficiency; Arabidopsis; Cisgenic; MYB61 transcription factor; Citrus
Abstract Water-use efficiency (WUE) is a quantitative measurement of biomass produced per volume of water transpired by a plant. WUE is an important physiological trait for drought response to mitigate the water deficiency. In this work, a cisgenic construction from Citrus sinensis was developed and its function in the improvement of WUE was evaluated in Arabidopsis. Sequences of the CsMYB61 coding region, a transcription factor implicated in the closure of stomata, together with a putative stomata-specific promoter from CsMYB.1.5, were identified and cloned. The protein encoded in the CsMYB61 locus harbors domains and motifs characteristic of MYB61 proteins. In addition, a 1.2 kb promoter region of the gene CsMYB15 (pCsMYB15) containing regulatory elements for expression in guard cells and in response to Abscisic Acid (ABA) and light was isolated. In Arabidopsis, pCsMYB15 directs the expression of the reporter gene GUS in stomata in the presence of light. In addition, transgenic lines expressing the CsMYB61 coding region under transcriptional control of pCsMYB15 have a normal phenotype under in vitro and greenhouse conditions. These transgenic lines exhibited a smaller opening of the stomata pore, lower stomatal conductance and respiration rate, enhanced sensitivity to exogenous ABA, and high drought stress tolerance. Our results indicate that stomata-specific expression of CsMYB61 enhances water use efficiency under drought conditions in Arabidospis.
Address [Romero-Romero, Jesus L.; Orellana, Daniela; Pablo Matte, Juan; Arce-Johnson, Patricio] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Genet Mol & Microbiol, Santiago, Chile, Email: parce@bio.puc.cl
Corporate Author Thesis
Publisher Elsevier France-Editions Scientifiques Medicales Elsevier Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0981-9428 ISBN Medium
Area Expedition Conference
Notes WOS:000444789200006 Approved
Call Number UAI @ eduardo.moreno @ Serial 915
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Author Romero-Romero, J.L.; Inostroza-Blancheteau, C.; Reyes-D?az, M.; Matte, J.P.; Aquea, F.; Espinoza, C.; Gil, P.M.; Arce-johnson, P.
Title Increased Drought and Salinity Tolerance in Citrus aurantifolia (Mexican Lemon) Plants Overexpressing Arabidopsis CBF3 Gene Type
Year 2020 Publication Journal Of Soil Science And Plant Nutrition Abbreviated Journal J. Soil Sci. Plant Nutr.
Volume 20 Issue 1 Pages 244-252
Keywords Drought tolerance; Abiotic stress; Transgenics citrus; CBF3 transcription factor
Abstract Citrus are a globally important fruit crop. Abiotic stressors such as drought and salinity adversely affect physiological citrus performance and survival. With the aim of improving drought tolerance in citrus plants, we constructed transgenic lines of Citrus lemon overexpressing the Arabidopsis transcription factor CBF3. Molecular, physiological, and quantitative real-time analyses showed high expression of AtCBF3 in three selected transgenic lines. During a 15-day treatment of water deficit by cessation of irrigation, the transgenic lines LM2 and LM14 showed lower stomatal conductance and transpiration paired with lower photosynthesis, whereas transgenic line LM7 maintained its photosynthesis, declining stomatal conductance, and transpiration compared to WT plants, which is manifested into more efficient water use. The genes CsRafS1 and CsGolS1 showed similar or greater expression in one of the transgenic lines with respect to control plants. Moreover, transgenic lines were more tolerant to saline stress and presented a greener phenotype with increased chlorophyll content in leaf discs compared to WT plants. In addition, a lower electrical conductivity in solution was observed in transgenic lines. Furthermore, all transgenic lines exhibited significantly less accumulation of reactive oxygen species than WT plants. Together, these results suggest the potential for heterologous expression of the AtCBF3 gene to mediate tolerance to hydric and saline stress in citrus plants.
Address [Romero-Romero, Jesus L.] CIIIDIR, Inst Politecn Nacl, Dept Biotecnol Agr, Unidad Sinaloa, Guasave, Sinaloa, Mexico, Email: parce@bio.puc.cl
Corporate Author Thesis
Publisher Springer International Publishing Ag Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0718-9508 ISBN Medium
Area Expedition Conference
Notes WOS:000520138000021 Approved
Call Number UAI @ eduardo.moreno @ Serial 1158
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