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Author Govindan, R.; Gnanasekaran, C.; Govindan, R.; Muthuchamy, M.; Quero, F.; Jothi, A.; Chelliah, C.K.; Arunachalam, A.; Mangalaraja, R.V.; Natesan, M,; Kadaikunnan, S,; Li, W.J.
Title Anti-quorum Sensing and Anti-biofilm Effect of Nocardiopsis synnemataformans RMN 4 (MN061002) Compound 2,6-Di-tert-butyl, 1,4-Benzoquinone Against Biofilm-Producing Bacteria Type
Year 2023 Publication Applied Biochemistry And Biotechnology Abbreviated Journal Appl. Biochem. Biotechnol.
Volume Early Access Issue Pages
Keywords Marine actinomycetes; Spectroscopic analysis, HPLC purification, Anti-QS activity; Anti-biofilm activity; Scanning electron microscopy
Abstract In this study, the anti-biofilm compound of 2,6-Di-tert-butyl, 1,4-benzoquinone was purified from Nocardiopsis synnemataformans (N. synnemataformans) RMN 4 (MN061002). To confirm the compound, various spectroscopy analyses were done including ultraviolet (UV) spectrometer, Fourier transform infrared spectroscopy (FTIR), analytical high-performance liquid chromatography (HPLC), preparative HPLC, gas chromatography-mass spectroscopy (GC-MS), liquid chromatography-mass spectroscopy (LC-MS), and 2D nuclear magnetic resonance (NMR). Furthermore, the purified compound was shown 94% inhibition against biofilm-producing Proteus mirabilis (P. mirabilis) (MN396686) at 70 mu g/mL concentrations. Furthermore, the metabolic activity, exopolysaccharide damage, and hydrophobicity degradation results of identified compound exhibited excellent inhibition at 100 mu g/mL concentration. Furthermore, the confocal laser scanning electron microscope (CLSM) and scanning electron microscope (SEM) results were shown with intracellular damages and architectural changes in bacteria. Consecutively, the in vivo toxicity effect of the compound against Artemia franciscana (A. franciscana) was shown to have a low mortality rate at 100 mu g/mL. Finally, the molecular docking interaction between the quorum sensing (QS) genes and identified compound clearly suggested that the identified compound 2,6-Di-tert-butyl, 1,4-benzoquinone has anti-quorum sensing and anti-biofilm activities against P. mirabilis (MN396686).
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ISSN 0273-2289 ISBN Medium
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Notes WOS:001105945900003 Approved
Call Number UAI @ alexi.delcanto @ Serial 1931
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