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Author Aquea, F.; Timmermann, T.; Herrera-Vasquez, A.
Title Chemical inhibition of the histone acetyltransferase activity in Arabidopsis thaliana Type
Year 2017 Publication Biochemical And Biophysical Research Communications Abbreviated Journal Biochem. Biophys. Res. Commun.
Volume (down) 483 Issue 1 Pages 664-668
Keywords Histone acetyltransferase; GCN5; Chemical inhibitor; Chromatin remodeling; Arabidopsis
Abstract Chemical inhibition of chromatin regulators provides an effective approach to investigate the roles of chromatin modifications in plant and animals. In this work, chemical inhibition of the Arabidopsis histone acetyltransferase activity by gamma-butyrolactone (MB-3), the inhibitor of the catalytic activity of mammalian GENERAL CONTROL NON-REPRESSIBLE 5 (GCN5) is evaluated. Arabidopsis seedlings were germinated in LS medium supplemented with different concentrations of MB-3, and inhibition in the root length and yellowed leaves were observed. The yellowed leaves phenotype of the plants grown in 100 μM of MB-3 was reverted when plants were additionally treated with 1 μM of TSA, a histone deacetylase inhibitor. Using an immunoblot assay with specific antibodies revealed a reduction of H3K14 acetylation levels at 3 and 24 h post-treatment. At 24 h post-treatment a reduction of H3K9 acetylation levels was observed. Targets of GCN5 related to stress were downregulated at 3 h post-treatment but no change was observed in target genes related to developmental transition. Our results indicate that MB-3 is a chemical inhibitor of the histone acetyltransferase in Arabidopsis and suggest that this inhibitor could function in other plants species. (C) 2016 Elsevier Inc. All rights reserved.
Address [Aquea, Felipe; Timmermann, Tania] Univ Adolfo Ibanez, Fac Ingn & Ciencias, Lab Bioingn, Santiago, Chile, Email: felipe.aquea@uai.cl
Corporate Author Thesis
Publisher Academic Press Inc Elsevier Science Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0006-291x ISBN Medium
Area Expedition Conference
Notes WOS:000397259000103 Approved
Call Number UAI @ eduardo.moreno @ Serial 711
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Author Aquea, F.; Vega, A.; Timmermann, T.; Poupin, M.J.; Arce-Johnson, P.
Title Genome-wide analysis of the SET DOMAIN GROUP family in Grapevine Type
Year 2011 Publication Plant Cell Reports Abbreviated Journal Plant Cell Reports
Volume (down) 30 Issue 6 Pages 1087-1097
Keywords Grapes; Vitis vinifera; Chromatin remodeling; Histone methyltransferase
Abstract The SET DOMAIN GROUP (SDG) proteins represent an evolutionarily-conserved family of epigenetic regulators present in eukaryotes and are putative candidates for the catalysis of lysine methylation in histones. Plant genomes analyses of this family have been performed in arabidopsis, maize, and rice and functional studies have shown that SDG genes are involved in the control of plant development. In this work, we describe the identification and structural characterization of SDG genes in the Vitis vinifera genome. This analysis revealed the presence of 33 putative SDG genes that can be grouped into different classes, as it has been previously described for plants. In addition to the SET domain, the proteins identified possessed other domains in the different classes. As part of our study regarding the growth and development of grapevine, we selected eight genes and their expression levels were analyzed in representative vegetative and reproductive organs of this species. The selected genes showed different patterns of expression during inflorescence and fruit development, suggesting that they participate in these processes. Furthermore, we showed that the expression of selected SDGs changes during viral infection, using as a model Grapevine Leafroll Associated Virus 3-infected symptomatic grapevine leaves and fruits. Our results suggest that developmental changes caused by this virus could be the result of alterations in SDG expression.
Address [Aquea, Felipe; Arce-Johnson, Patricio] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Genet Mol & Microbiol, Santiago, Chile, Email: parce@bio.puc.cl
Corporate Author Thesis
Publisher Springer Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0721-7714 ISBN Medium
Area Expedition Conference
Notes WOS:000290542800013 Approved
Call Number UAI @ eduardo.moreno @ Serial 184
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Author Moraga, F.; Aquea, F.
Title Composition of the SAGA complex in plants and its role in controlling gene expression in response to abiotic stresses Type
Year 2015 Publication Frontiers In Plant Science Abbreviated Journal Front. Plant Sci.
Volume (down) 6 Issue Pages 9 pp
Keywords SAGA complex; chromatin remodeling; transcriptional coactivator; abiotic stress; protein complex; histone acetyltransferase
Abstract Protein complexes involved in epigenetic regulation of transcription have evolved as molecular strategies to face environmental stress in plants. SAGA (Spt-Ada-Gcn5 Acetyltransferase) is a transcriptional co-activator complex that regulates numerous cellular processes through the coordination of multiple post-translational histone modifications, including acetylation, deubiquitination, and chromatin recognition. The diverse functions of the SAGA complex involve distinct modules that are highly conserved between yeast, flies, and mammals. In this review, the composition of the SAGA complex in plants is described and its role in gene expression regulation under stress conditions summarized. Some of these proteins are likely involved in the regulation of the inducible expression of genes under light, cold, drought, salt, and iron stress, although the functions of several of its components remain unknown.
Address [Moraga, Felipe; Aquea, Felipe] Univ Adolfo Ibanez, Fac Ingn & Ciencias, Lab Bioingn, Santiago, Chile, Email: felipe.aquea@uai.cl
Corporate Author Thesis
Publisher Frontiers Media Sa Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 1664-462x ISBN Medium
Area Expedition Conference
Notes WOS:000364240600001 Approved
Call Number UAI @ eduardo.moreno @ Serial 540
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