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Author Collins, C.J.; Vivanco, J.F.; Sokn, S.A.; Williams, B.O.; Burgers, T.A.; Ploeg, H.L.
Title Fracture healing in mice lacking Pten in osteoblasts: a micro-computed tomography image-based analysis of the mechanical properties of the femur Type
Year 2015 Publication Journal Of Biomechanics Abbreviated Journal J. Biomech.
Volume 48 Issue 2 Pages 310-317
Keywords Fracture healing; Mouse femur; Pten gene; Micro computed tomography image-based analysis; Section properties; Mechanical properties; Four-point bend testing
Abstract In the United States, approximately eight million osseous fractures are reported annually, of which 5-10% fail to create a bony union. Osteoblast-specific deletion of the gene Pten in mice has been found to stimulate bone growth and accelerate fracture healing. Healing rates at four weeks increased in femurs from Pten osteoblast conditional knock-out mice (Pten-CKO) compared to wild-type mice (WT) of the same genetic strain as measured by an increase in mechanical stiffness and failure load in four-point bending tests. Preceding mechanical testing, each femur was imaged using a Skyscan 1172 micro-computed tomography (mu CT) scanner (Skyscan, Kontich, Belgium). The present study used μCT image-based analysis to test the hypothesis that the increased femoral fracture force and stiffness in Pten-CKO were due to greater section properties with the same effective material properties as that of the WT. The second moment of area and section modulus were computed in ImageJ 1.46 (National Institutes of Health) and used to predict the effective flexural modulus and the stress at failure for fourteen pairs of intact and callus WT and twelve pairs of intact and callus Pten-CKO femurs. For callus and intact femurs, the failure stress and tissue mineral density of the Pten-CKO and WT were not different; however, the section properties of the Pten-CKO were more than twice as large 28 days post-fracture. It was therefore concluded, when the gene Pten was conditionally knocked-out in osteoblasts, the resulting increased bending stiffness and force to fracture were due to increased section properties. (C) 2014 Elsevier Ltd. All rights reserved.
Address [Collins, Caitlyn J.; Vivanco, Juan F.; Sokn, Scott A.; Ploeg, Heidi-Lynn] Univ Wisconsin, Dept Mech Engn, Madison, WI 53706 USA, Email: ploeg@engr.wisc.edu
Corporate Author Thesis
Publisher (up) Elsevier Sci Ltd Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0021-9290 ISBN Medium
Area Expedition Conference
Notes WOS:000349194800018 Approved
Call Number UAI @ eduardo.moreno @ Serial 454
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