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Author Besaury, L.; Ouddane, B.; Pavissich, J.P.; Dubrulle-Brunaud, C.; Gonzalez, B.; Quillet, L.
Title Impact of copper on the abundance and diversity of sulfate-reducing prokaryotes in two chilean marine sediments Type
Year 2012 Publication Marine Pollution Bulletin Abbreviated Journal Mar. Pollut. Bull.
Volume 64 Issue 10 Pages 2135-2145
Keywords Marine sediment; Copper contamination; Sulfate-reducing prokaryotes; Real-time PCR; DGGE; Phylogenetic analysis
Abstract We studied the abundance and diversity of the sulfate-reducing prokaryotes (SRPs) in two 30-cm marine chilean sediment cores, one with a long-term exposure to copper-mining residues, the other being a non-exposed reference sediment. The abundance of SRPs was quantified by qPCR of the dissimilatory sulfite reductase gene beta-subunit (dsrB) and showed that SRPs are sensitive to high copper concentrations, as the mean number of SRPs all along the contaminated sediment was two orders of magnitude lower than in the reference sediment. SRP diversity was analyzed by using the dsrB-sequences-based PCR-DGGE method and constructing gene libraries for dsrB-sequences. Surprisingly, the diversity was comparable in both sediments, with dsrB sequences belonging to Desulfobacteraceae, Syntrophobacteraceae, and Desulfobulbaceae, SRP families previously described in marine sediments, and to a deep branching dsrAB lineage. The hypothesis of the presence of horizontal transfer of copper resistance genes in the microbial population of the polluted sediment is discussed. (C) 2012 Elsevier Ltd. All rights reserved.
Address [Besaury, Ludovic; Quillet, Laurent] Univ Rouen, Lab Univ MEBE Microbiol Environm & Biol Evolut, F-76821 Mont St Aignan, France, Email: ludovic.besaury@etu.univ-rouen.fr;
Corporate Author Thesis
Publisher Pergamon-Elsevier Science Ltd Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0025-326x ISBN Medium
Area Expedition Conference
Notes WOS:000310929500032 Approved
Call Number UAI @ eduardo.moreno @ Serial 254
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Author Martinez, J.; Simon, V.; Gonzalez, B.; Conget, P.
Title A real-time PCR-based strategy for the detection of Paenibacillus larvae vegetative cells and spores to improve the diagnosis and the screening of American foulbrood Type
Year 2010 Publication Letters In Applied Microbiology Abbreviated Journal Lett. Appl. Microbiol.
Volume 50 Issue 6 Pages 603-610
Keywords American foulbrood; flow cytometry; Paenibacillus larvae; real-time PCR; 16S rRNA specific primers
Abstract Aim: To develop a real-time PCR-based strategy for the detection of Paenibacillus larvae vegetative cells and spores to improve the diagnosis and the screening of American foulbrood (AFB), the most harmful pathology of honeybee brood. Methods and Results: A real-time PCR that allowed selective identification and quantification of P. larvae 16S rRNA sequence was developed. Using standard samples quantified by flow cytometry, detection limits of 37 center dot 5 vegetative cells ml-1 and 10 spores ml-1 were determined. Compared to spread plate method, this real-time PCR-based strategy allowed, in only 2 h, the detection of P. larvae in contaminated honeys. No false-positive results were obtained. Moreover, its detection limit was 100 times lower than that of the culture method (2 vs 200 spores g-1 of honey). Conclusion: A rapid, selective, with low detection limit, sensitive and specific method to detect and quantify vegetative cells and spores of P. larvae is now available. Significance and Impact of Study: In addition to honey samples, this real-time PCR-based strategy may be also applied to confirm AFB diagnosis in honeybee brood and to screen other apiary supplies and products (bees, pollen, wax), thus broadening the control of AFB spreading.
Address [Martinez, J.; Simon, V.; Conget, P.] Clin Alemana Univ Desarrollo, Fac Med, Inst Ciencias, Santiago 7710162, Chile, Email: pconget@udd.cl
Corporate Author Thesis
Publisher Wiley-Blackwell Publishing, Inc Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0266-8254 ISBN Medium
Area Expedition Conference
Notes WOS:000277417000009 Approved
Call Number UAI @ eduardo.moreno @ Serial 89
Permanent link to this record