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Author |
De la Iglesia, R.; Valenzuela-Heredia, D.; Pavissich, J.P.; Freyhoffer, S.; Andrade, S.; Correa, J.A.; Gonzalez, B. |
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Title |
Novel polymerase chain reaction primers for the specific detection of bacterial copper P-type ATPases gene sequences in environmental isolates and metagenomic DNA |
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Year |
2010 |
Publication |
Letters In Applied Microbiology |
Abbreviated Journal |
Lett. Appl. Microbiol. |
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Volume |
50 |
Issue |
6 |
Pages |
552-562 |
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Keywords |
ATPases; bacterial communities; copper resistance; metagenomic DNA; PCR primer pair |
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Abstract |
Aims: In the last decades, the worldwide increase in copper wastes release by industrial activities like mining has driven environmental metal contents to toxic levels. For this reason, the study of the biological copper-resistance mechanisms in natural environments is important. Therefore, an appropriate molecular tool for the detection and tracking of copper-resistance genes was developed. Methods and Results: In this work, we designed a PCR primer pair to specifically detect copper P-type ATPases gene sequences. These PCR primers were tested in bacterial isolates and metagenomic DNA from intertidal marine environments impacted by copper pollution. As well, T-RFLP fingerprinting of these gene sequences was used to compare the genetic composition of such genes in microbial communities, in normal and copper-polluted coastal environments. New copper P-type ATPases gene sequences were found, and a high degree of change in the genetic composition because of copper exposure was also determined. Conclusions: This PCR based method is useful to track bacterial copper-resistance gene sequences in the environment. Significance and Impact of the Study: This study is the first to report the design and use of a PCR primer pair as a molecular marker to track bacterial copper-resistance determinants, providing an excellent tool for long-term analysis of environmental communities exposed to metal pollution. |
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Address |
[Gonzalez, B.] Univ Adolfo Ibanez, Fac Ingn & Ciencia, Santiago 7941169, Chile, Email: bernardo.gonzalez@uai.cl |
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Wiley-Blackwell Publishing, Inc |
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English |
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0266-8254 |
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WOS:000277417000002 |
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UAI @ eduardo.moreno @ |
Serial |
88 |
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Gazitua, M.C.; Morgante, V.; Poupin, M.J.; Ledger, T.; Rodriguez-Valdecantos, G.; Herrera, C.; Gonzalez-Chavez, M.D.; Ginocchio, R.; Gonzalez, B. |
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Title |
The microbial community from the early-plant colonizer (Baccharis linearis) is required for plant establishment on copper mine tailings |
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Year |
2021 |
Publication |
Scientific Reports |
Abbreviated Journal |
Sci. Rep. |
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Volume |
11 |
Issue |
1 |
Pages |
10448 |
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Keywords |
BACTERIAL COMMUNITIES; HEAVY-METALS; PHYTOSTABILIZATION; REVEGETATION; RHIZOSPHERE; REMEDIATION; IMPACT; GROWTH; NORTH |
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Abstract |
Plants must deal with harsh environmental conditions when colonizing abandoned copper mine tailings. We hypothesized that the presence of a native microbial community can improve the colonization of the pioneer plant, Baccharis linearis, in soils from copper mining tailings. Plant growth and microbial community compositions and dynamics were determined in cultivation pots containing material from two abandoned copper mining tailings (Huana and Tambillos) and compared with pots containing fresh tailings or surrounding agricultural soil. Controls without plants or using irradiated microbe-free substrates, were also performed. Results indicated that bacteria (Actinobacteria, Gammaproteobacteria, and Firmicutes groups) and fungi (Glomus genus) are associated with B. linearis and may support plant acclimation, since growth parameters decreased in both irradiated (transiently without microbial community) and fresh tailing substrates (with a significantly different microbial community). Consistently, the composition of the bacterial community from abandoned copper mining tailings was more impacted by plant establishment than by differences in the physicochemical properties of the substrates. Bacteria located at B. linearis rhizoplane were clearly the most distinct bacterial community compared with those of fresh tailings, surrounding soil and non-rhizosphere abandoned tailings substrates. Beta diversity analyses showed that the rhizoplane bacterial community changed mainly through species replacement (turnover) than species loss (nestedness). In contrast, location/geographical conditions were more relevant than interaction with the plants, to explain fungal community differences. |
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2045-2322 |
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WOS:000658433400011 |
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UAI @ alexi.delcanto @ |
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1425 |
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