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Author Besaury, L.; Ouddane, B.; Pavissich, J.P.; Dubrulle-Brunaud, C.; Gonzalez, B.; Quillet, L. pdf  doi
openurl 
  Title Impact of copper on the abundance and diversity of sulfate-reducing prokaryotes in two chilean marine sediments Type Journal Article
  Year 2012 Publication Marine Pollution Bulletin Abbreviated Journal Mar. Pollut. Bull.  
  Volume 64 Issue 10 Pages 2135-2145  
  Keywords Marine sediment; Copper contamination; Sulfate-reducing prokaryotes; Real-time PCR; DGGE; Phylogenetic analysis  
  Abstract We studied the abundance and diversity of the sulfate-reducing prokaryotes (SRPs) in two 30-cm marine chilean sediment cores, one with a long-term exposure to copper-mining residues, the other being a non-exposed reference sediment. The abundance of SRPs was quantified by qPCR of the dissimilatory sulfite reductase gene beta-subunit (dsrB) and showed that SRPs are sensitive to high copper concentrations, as the mean number of SRPs all along the contaminated sediment was two orders of magnitude lower than in the reference sediment. SRP diversity was analyzed by using the dsrB-sequences-based PCR-DGGE method and constructing gene libraries for dsrB-sequences. Surprisingly, the diversity was comparable in both sediments, with dsrB sequences belonging to Desulfobacteraceae, Syntrophobacteraceae, and Desulfobulbaceae, SRP families previously described in marine sediments, and to a deep branching dsrAB lineage. The hypothesis of the presence of horizontal transfer of copper resistance genes in the microbial population of the polluted sediment is discussed. (C) 2012 Elsevier Ltd. All rights reserved.  
  Address [Besaury, Ludovic; Quillet, Laurent] Univ Rouen, Lab Univ MEBE Microbiol Environm & Biol Evolut, F-76821 Mont St Aignan, France, Email: ludovic.besaury@etu.univ-rouen.fr;  
  Corporate Author Thesis  
  Publisher Pergamon-Elsevier Science Ltd Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0025-326x ISBN Medium  
  Area Expedition Conference  
  Notes WOS:000310929500032 Approved no  
  Call Number UAI @ eduardo.moreno @ Serial 254  
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Author De la Iglesia, R.; Valenzuela-Heredia, D.; Andrade, S.; Correa, J.; Gonzalez, B. pdf  doi
openurl 
  Title Composition dynamics of epilithic intertidal bacterial communities exposed to high copper levels Type Journal Article
  Year 2012 Publication Fems Microbiology Ecology Abbreviated Journal FEMS Microbiol. Ecol.  
  Volume 79 Issue 3 Pages 720-727  
  Keywords copA; copper; epilithic bacteria; intertidal rocky shore; T-RFLP  
  Abstract Copper has a dual role for organisms, both as micronutrient and toxic element. Copper mining activities have an enormous ecological impact because of the extraction process and the consequent release of copper-containing waste materials to the environment. In northern Chile, mainly in the Chanaral coastal area, this phenomenon is clearly evident. The released waste material has caused a strong modification of the area, and copper enrichment of beaches and rocky shores has provoked a decrease in the richness and diversity of many species of macroorganisms. However, the effects that copper enrichment has on microbial (e.g. bacterial epilithic) communities associated with the rocky shore environment are poorly understood. Using a culture-independent molecular approach, field sampling and laboratory microcosm experiments, we determined the effects of copper enrichment on bacterial communities inhabiting the rocky shore environment. Field samples showed a strong effect of copper on the structure of the natural bacterial epilithic communities, and microcosm experiments demonstrated rapid changes in bacterial community when copper is added, and reversibility of this effect within 48 h after copper is removed.  
  Address [Gonzalez, Bernardo] Univ Adolfo Ibanez, Fac Ingn & Ciencias, Santiago 7941169, Chile, Email: bernardo.gonzalez@uai.cl  
  Corporate Author Thesis  
  Publisher Wiley-Blackwell Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0168-6496 ISBN Medium  
  Area Expedition Conference  
  Notes WOS:000299257300014 Approved no  
  Call Number UAI @ eduardo.moreno @ Serial 192  
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Author De la Iglesia, R.; Valenzuela-Heredia, D.; Pavissich, J.P.; Freyhoffer, S.; Andrade, S.; Correa, J.A.; Gonzalez, B. pdf  doi
openurl 
  Title Novel polymerase chain reaction primers for the specific detection of bacterial copper P-type ATPases gene sequences in environmental isolates and metagenomic DNA Type Journal Article
  Year 2010 Publication Letters In Applied Microbiology Abbreviated Journal Lett. Appl. Microbiol.  
  Volume 50 Issue 6 Pages 552-562  
  Keywords ATPases; bacterial communities; copper resistance; metagenomic DNA; PCR primer pair  
  Abstract Aims: In the last decades, the worldwide increase in copper wastes release by industrial activities like mining has driven environmental metal contents to toxic levels. For this reason, the study of the biological copper-resistance mechanisms in natural environments is important. Therefore, an appropriate molecular tool for the detection and tracking of copper-resistance genes was developed. Methods and Results: In this work, we designed a PCR primer pair to specifically detect copper P-type ATPases gene sequences. These PCR primers were tested in bacterial isolates and metagenomic DNA from intertidal marine environments impacted by copper pollution. As well, T-RFLP fingerprinting of these gene sequences was used to compare the genetic composition of such genes in microbial communities, in normal and copper-polluted coastal environments. New copper P-type ATPases gene sequences were found, and a high degree of change in the genetic composition because of copper exposure was also determined. Conclusions: This PCR based method is useful to track bacterial copper-resistance gene sequences in the environment. Significance and Impact of the Study: This study is the first to report the design and use of a PCR primer pair as a molecular marker to track bacterial copper-resistance determinants, providing an excellent tool for long-term analysis of environmental communities exposed to metal pollution.  
  Address [Gonzalez, B.] Univ Adolfo Ibanez, Fac Ingn & Ciencia, Santiago 7941169, Chile, Email: bernardo.gonzalez@uai.cl  
  Corporate Author Thesis  
  Publisher Wiley-Blackwell Publishing, Inc Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0266-8254 ISBN Medium  
  Area Expedition Conference  
  Notes WOS:000277417000002 Approved no  
  Call Number UAI @ eduardo.moreno @ Serial 88  
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Author Parrado, C.; Caceres, G.; Bize, F.; Bubnovich, V.; Baeyens, J.; Degreve, J.; Zhang, H.L. pdf  doi
openurl 
  Title Thermo-mechanical analysis of copper-encapsulated NaNO3-KNO3 Type Journal Article
  Year 2015 Publication Chemical Engineering Research & Design Abbreviated Journal Chem. Eng. Res. Des.  
  Volume 93 Issue Pages 224-231  
  Keywords Copper-encapsulation; Nitrate salts; Simulation; Phase change material; Thermal energy storage; Comsol Multiphysics  
  Abstract The present paper presents a numerical study to investigate and assess the heat transfer behavior of a copper and salt composite. A mixture of nitrates, KNO3-NaNO3, within a deformable spherical shell coating of copper will be used as an encapsulated phase change material, E-PCM. In the context of a thermo-mechanical analysis of this E-PCM, a simulation is proposed to determine its storage capacity and properties The melting, or solidification of the encapsulated PCM particles do not provoke cracking of the deformable shell. (C) 2014 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.  
  Address [Parrado, C.; Caceres, G.; Bize, F.; Bubnovich, V.] Univ Adolfo Ibanez, Fac Sci & Engn, Santiago, Chile, Email: J.Baeyens@warwick.ac.uk  
  Corporate Author Thesis  
  Publisher Inst Chemical Engineers Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0263-8762 ISBN Medium  
  Area Expedition Conference  
  Notes WOS:000348878600021 Approved no  
  Call Number UAI @ eduardo.moreno @ Serial 457  
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Author Pavissich, J.P.; Silva, M.; Gonzalez, B. pdf  doi
openurl 
  Title Sulfate Reduction, Molecular Diversity, And Copper Amendment Effects In Bacterial Communities Enriched From Sediments Exposed To Copper Mining Residues Type Journal Article
  Year 2010 Publication Environmental Toxicology And Chemistry Abbreviated Journal Environ. Toxicol. Chem.  
  Volume 29 Issue 2 Pages 256-264  
  Keywords Sulfate reduction; Coastal sediments; Sulfate-reducing bacteria; Copper; Molecular diversity  
  Abstract Sulfate-reducing bacterial communities from coastal sediments with a long-term exposure to copper (Cu)-mining residues were studied in lactate enrichments. The toxicity of excess copper may affect sulfate-reducing bacterial communities. Sulfate reduction was monitored by sulfate and organic acid measurements. Molecular diversity was analyzed by 16S rRNA, dissimilatory sulfate reduction dsrAB, and Cu translocating phospho-type adenosine triphosphatases (P-ATPases) cop-like gene sequence profiling. The influence of Cu amendment was tested in these enrichments. Results showed fast sulfate reduction mostly coupled to incomplete organic carbon oxidation and partial sulfate reduction inhibition due to copper amendment. The 16S rRNA clonal libraries analysis indicated that delta- and gamma-Proteobacteria and Cytophaga-Flexibacter-Bacteroides dominated the enrichments. The dsrAB libraries revealed the presence of Desulfobacteraceae and Desulfovibrionaceae families-related sequences. Copper produced significant shifts (i.e., a decrease in the relative abundance of sulfate-reducing microorganisms) in the enriched bacterial community structure as determined by terminal-restriction fragment length polymorphism (T-RFLP) profiling and multivariate analyses. Clonal libraries of cop-like sequences showed low richness in the enriched microbial communities, and a strong effect of copper on its relative abundance. Novel Cu-P-IB-ATPase sequences encoding Cu resistance were detected. The present study indicates that Cu does not significantly affect sulfate reduction and genetic diversity of taxonomic and dissimilatory sulfate-reduction molecular markers. However, the diversity of Cu resistance genetic determinants was strongly modified by this toxic metal. Environ. Toxicol. Chem. 2010;29:256-264. (C) 2009 SETAC  
  Address [Pavissich, Juan P.; Silva, Macarena; Gonzalez, Bernardo] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Millennium Nucleus Microbial Ecol & Environm Micr, Dept Mol Genet & Microbiol,CASEB, Santiago, Chile, Email: bernardo.gonzalez@uai.cl  
  Corporate Author Thesis  
  Publisher Setac Press Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0730-7268 ISBN Medium  
  Area Expedition Conference  
  Notes WOS:000274272500004 Approved no  
  Call Number UAI @ eduardo.moreno @ Serial 81  
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Author Pavissich, J.P.; Vargas, I.T.; Gonzalez, B.; Pasten, P.A.; Pizarro, G.E. pdf  doi
openurl 
  Title Culture dependent and independent analyses of bacterial communities involved in copper plumbing corrosion Type Journal Article
  Year 2010 Publication Journal Of Applied Microbiology Abbreviated Journal J. Appl. Microbiol.  
  Volume 109 Issue 3 Pages 771-782  
  Keywords bacterial community; biofilm; copper plumbing; microbially influenced corrosion; water quality  
  Abstract Aims: This study used culture-dependent and culture-independent approaches to characterize bacterial communities in copper plumbing corrosion and to assess biofilm formation and copper resistance of heterotrophic bacteria isolated from copper pipes. Methods and Results: Water and copper pipes were collected from a cold-water household distribution system affected by 'blue water' corrosion and presenting biofilm formation. Corrosion-promoting ageing experiments were performed with conditioned unused copper pipes filled with unfiltered and filtered sampled water as nonsterile and sterile treatments, respectively. During 8 weeks, stagnant water within the pipes was replaced with aerated fresh water every 2 or 3 days. Total copper and pH were determined in sampled water, and copper pipe coupons were cut for microscopic analyses. Biofilms were extracted from field and laboratory pipes, and total DNA was isolated. Bacterial communities' composition was analysed by terminal restriction fragment length polymorphism (T-RFLP) and clonal libraries of 16S rRNA genes. Heterotrophic bacterial isolates were obtained from water and biofilm extracts and characterized in terms of biofilm formation capacity and copper minimum inhibitory concentration. The results indicated that copper concentration in stagnant water from nonsterile treatments was much higher than in sterile treatments and corrosion by-products structure in coupon surfaces was different. Multivariate analysis of T-RFLP profiles and clone sequencing showed significant dissimilarity between field and laboratory biofilm communities, and a low richness and the dominant presence of Gamma- and Betaproteobacteria in both cases. Several bacterial isolates formed biofilm and tolerated high copper concentrations. Conclusions: The study demonstrates microbially influenced corrosion (MIC) in copper plumbing. Gamma- and Betaproteobacteria dominated the corroded copper piping bacterial community, whose ability to form biofilms may be important for bacterial corrosion promotion and survival in MIC events. Significance and Impact of the Study: The characterization of micro-organisms that influence copper plumbing corrosion has significant implications for distribution system management and copper corrosion control.  
  Address [Pavissich, J. P.; Vargas, I. T.; Pasten, P. A.; Pizarro, G. E.] Pontificia Univ Catolica Chile, Dept Ingn Hidraul & Ambiental, Escuela Ingn, Santiago 6904411, Chile, Email: gpizarro@ing.puc.cl  
  Corporate Author Thesis  
  Publisher Wiley-Blackwell Publishing, Inc Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1364-5072 ISBN Medium  
  Area Expedition Conference  
  Notes WOS:000280979700004 Approved no  
  Call Number UAI @ eduardo.moreno @ Serial 93  
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Author Vargas, I.T.; Fischer, D.A.; Alsina, M.A.; Pavissich, J.P.; Pasten, P.A.; Pizarro, G.E. pdf  doi
openurl 
  Title Copper Corrosion and Biocorrosion Events in Premise Plumbing Type Journal Article
  Year 2017 Publication Materials Abbreviated Journal Materials  
  Volume 10 Issue 9 Pages 30 pp  
  Keywords copper; corrosion; biocorrosion; drinking water; premise plumbing; MIC  
  Abstract Corrosion of copper pipes may release high amounts of copper into the water, exceeding the maximum concentration of copper for drinking water standards. Typically, the events with the highest release of copper into drinking water are related to the presence of biofilms. This article reviews this phenomenon, focusing on copper ingestion and its health impacts, the physicochemical mechanisms and the microbial involvement on copper release, the techniques used to describe and understand this phenomenon, and the hydrodynamic effects. A conceptual model is proposed and the mathematical models are reviewed.  
  Address [Vargas, Ignacio T.; Fischer, Diego A.; Alsina, Marco A.; Pasten, Pablo A.; Pizarro, Gonzalo E.] Pontificia Univ Catolica Chile, Dept Ingn Hidraul & Ambiental, Santiago 7820436, Chile, Email: itvargas@ing.puc.cl;  
  Corporate Author Thesis  
  Publisher Mdpi Ag Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1996-1944 ISBN Medium  
  Area Expedition Conference  
  Notes WOS:000411506700120 Approved no  
  Call Number UAI @ eduardo.moreno @ Serial 806  
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Author Villena, M.; Greve, F. pdf  doi
openurl 
  Title On resource depletion and productivity: The case of the Chilean copper industry Type Journal Article
  Year 2018 Publication Resources Policy Abbreviated Journal Resour. Policy  
  Volume 59 Issue Pages 553-562  
  Keywords Resource depletion; Technological change; Mining; Copper; Chile  
  Abstract How resource depletion affects productivity is a crucial question for several industries. In fact, several natural resource-exporting countries have seen their productivity levels affected by resource depletion. Nevertheless, usually, it is not clear what the real productivity growth is, without discarding the effects of resource depletion in the production structure. The main aim of the paper is to empirically answer a relevant issue regarding the Chilean copper mining industry, which is, the slowdown of its productivity in the last decade, considering in the analysis the role of resource depletion. In particular, we consider resource depletion to be an exogenous and unpaid force that opposes technological change and hence increases costs through time, capturing in this way some stylized facts of, for example, the mining and fishing industries. The decomposition framework was applied to the Chilean copper mining industry, one of the most important in the world, using data from the period of 1985-2015. The econometric results were robust and pointed to the fact that the productivity fell sharply during the period; however, it did not fall as much as the traditional estimation methods pointed out. Our model showed that as much as 15% of this decline was due to the increase of the resource depletion variable (copper ore grade).  
  Address [Villena, Marcelo] Univ Adolfo Ibanez, Diagonal Las Torres 2640, Santiago, Chile, Email: marcelo.villena@uai.cl;  
  Corporate Author Thesis  
  Publisher Elsevier Sci Ltd Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0301-4207 ISBN Medium  
  Area Expedition Conference  
  Notes WOS:000454974900054 Approved no  
  Call Number UAI @ eduardo.moreno @ Serial 970  
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