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Author Sundram, V.; Ng, F.S.; Roberts, M.A.; Millan, C.; Ewer, J.; Jackson, F.R. pdf  doi
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  Title Cellular Requirements for LARK in the Drosophila Circadian System Type
  Year 2012 Publication Journal Of Biological Rhythms Abbreviated Journal J. Biol. Rhythms  
  Volume 27 Issue 3 Pages 183-195  
  Keywords clock output; posttranscriptional; RNA binding; locomotor activity; eclosion  
  Abstract RNA-binding proteins mediate posttranscriptional functions in the circadian systems of multiple species. A conserved RNA recognition motif (RRM) protein encoded by the lark gene is postulated to serve circadian output and molecular oscillator functions in Drosophila and mammals, respectively. In no species, however, has LARK been eliminated, in vivo, to determine the consequences for circadian timing. The present study utilized RNA interference (RNAi) techniques in Drosophila to decrease LARK levels in clock neurons and other cell types in order to evaluate the circadian functions of the protein. Knockdown of LARK in timeless (TIM)- or pigment dispersing factor (PDF)-containing clock cells caused a significant number of flies to exhibit arrhythmic locomotor activity, demonstrating a requirement for the protein in pacemaker cells. There was no obvious effect on PER protein cycling in lark interference (RNAi) flies, but a knockdown within the PDF neurons was associated with increased PDF immunoreactivity at the dorsal termini of the small ventral lateral neuronal (s-LNv) projections, suggesting an effect on neuropeptide release. The expression of lark RNAi in multiple neurosecretory cell populations demonstrated that LARK is required within pacemaker and nonpacemaker cells for the manifestation of normal locomotor activity rhythms. Interestingly, decreased LARK function in the prothoracic gland (PG), a peripheral organ containing a clock required for the circadian control of eclosion, was associated with weak population eclosion rhythms or arrhythmicity.  
  Address [Sundram, Vasudha; Ng, Fanny S.; Roberts, Mary A.; Jackson, F. Rob] Tufts Univ, Sch Med, Dept Neurosci, Ctr Neurosci Res, Boston, MA 02111 USA, Email: rob.jackson@tufts.edu  
  Corporate Author Thesis  
  Publisher Sage Publications Inc Place of Publication Editor  
  Language English Summary Language Original Title (up)  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0748-7304 ISBN Medium  
  Area Expedition Conference  
  Notes WOS:000304715700001 Approved  
  Call Number UAI @ eduardo.moreno @ Serial 219  
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