|   | 
Author Pinedo, I.; Ledger, T.; Greve, M.; Poupin, M.J.
Title Burkholderia phytofirmans PsJN induces long-term metabolic and transcriptional changes involved in Arabidopsis thaliana salt tolerance Type
Year 2015 Publication Frontiers In Plant Science Abbreviated Journal Front. Plant Sci.
Volume 6 Issue Pages 17 pp
Keywords plant growth promoting rhizobacteria (PGPR); ion transport; osmotic stress response; priming; abiotic stress tolerance; reactive oxygen species (ROS); rhizosphere; beneficial bacteria
Abstract Salinity is one of the major limitations for food production worldwide. Improvement of plant salt-stress tolerance using plant-growth promoting rhizobacteria (PGPR) has arisen as a promising strategy to help overcome this limitation. However, the molecular and biochemical mechanisms controlling PGPR/plant interactions under salt-stress remain unclear. The main objective of this study was to obtain new insights into the mechanisms underlying salt-stress tolerance enhancement in the salt-sensitive Arabidopsis thaliana Col-0 plants, when inoculated with the well-known PGPR strain Burkholderia phytofirmans PsJN. To tackle this, different life history traits, together with the spatiotemporal accumulation patterns for key metabolites and salt-stress related transcripts, were analyzed in inoculated plants under short and long-term salt-stress. Inoculated plants displayed faster recovery and increased tolerance after sustained salt-stress. PsJN treatment accelerated the accumulation of proline and transcription of genes related to abscisic acid signaling (Relative to Dessication, RD29A and RD29B), ROS scavenging Oscorbate Peroxidase 2), and detoxification (Glyoxalase I 7), and down-regulated the expression of Lipoxygenase 2 (related to jasmonic acid biosynthesis). Among the general transcriptional effects of this bacterium, the expression pattern of important ion-homeostasis related genes was altered after short and longterm stress (Arabidopsis K Transporter 1, High-Affinity K Transporter 1, Sodium Hydrogen Exchanger 2, and Arabidopsis Salt Overly Sensitive 1). In all, the faster and stronger molecular changes induced by the inoculation suggest a PsJN-priming effect, which may explain the observed tolerance after short-term and sustained salt-stress in plants. This study provides novel information about possible mechanisms involved in salt-stress tolerance induced by PGPR in plants, showing that certain changes are maintained over time. This opens up new venues to study these relevant biological associations, as well as new approaches to a better understanding of the spatiotemporal mechanisms involved in stress tolerance in plants.
Address [Pinedo, Ignacio; Ledger, Thomas; Greve, Macarena; Poupin, Maria J.] Univ Adolfo Ibanez, Fac Ingn & Ciencias, Lab Bioingn, Santiago 7941169, Chile, Email: mpoupin@uai.cl
Corporate Author Thesis
Publisher Frontiers Research Foundation Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 1664-462x ISBN Medium
Area Expedition Conference
Notes WOS:000357301200001 Approved
Call Number UAI @ eduardo.moreno @ Serial 509
Permanent link to this record