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Author Ledger, T.; Zuniga, A.; Kraiser, T.; Dasencich, P.; Donoso, R.; Perez-Pantoja, D.; Gonzalez, B.
Title Aromatic compounds degradation plays a role in colonization of Arabidopsis thaliana and Acacia caven by Cupriavidus pinatubonensis JMP134 Type
Year (up) 2012 Publication Antonie Van Leeuwenhoek International Journal Of General And Molecular Microbiology Abbreviated Journal Antonie Van Leeuwenhoek
Volume 101 Issue 4 Pages 713-723
Keywords Acacia caven; Arabidopsis thaliana; Aromatic compounds; Cupriavidus pinatubonensis JMP134; Plant growth; Rhizosphere
Abstract Plant rhizosphere and internal tissues may constitute a relevant habitat for soil bacteria displaying high catabolic versatility towards xenobiotic aromatic compounds. Root exudates contain various molecules that are structurally related to aromatic xenobiotics and have been shown to stimulate bacterial degradation of aromatic pollutants in the rhizosphere. The ability to degrade specific aromatic components of root exudates could thus provide versatile catabolic bacteria with an advantage for rhizosphere colonization and growth. In this work, Cupriavidus pinatubonensis JMP134, a well-known aromatic compound degrader (including the herbicide 2,4-dichlorophenoxyacetate, 2,4-D), was shown to stably colonize Arabidopsis thaliana and Acacia caven plants both at the rhizoplane and endorhizosphere levels and to use root exudates as a sole carbon and energy source. No deleterious effects were detected on these colonized plants. When a toxic concentration of 2,4-D was applied to colonized A. caven, a marked resistance was induced in the plant, showing that strain JMP134 was both metabolically active and potentially beneficial to its host. The role for the beta-ketoadipate aromatic degradation pathway during plant root colonization by C. pinatubonensis JMP134 was investigated by gene inactivation. A C. pinatubonensis mutant derivative strain displayed a reduced ability to catabolise root exudates isolated from either plant host. In this mutant strain, a lower competence in the rhizosphere of A. caven was also shown, both in gnotobiotic in vitro cultures and in plant/soil microcosms.
Address [Ledger, Thomas; Zuniga, Ana; Dasencich, Paola; Donoso, Raul; Gonzalez, Bernardo] Univ Adolfo Ibanez, Fac Ingn & Ciencias, Santiago 7941169, Chile, Email: bernardo.gonzalez@uai.cl
Corporate Author Thesis
Publisher Springer Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0003-6072 ISBN Medium
Area Expedition Conference
Notes WOS:000303402400003 Approved
Call Number UAI @ eduardo.moreno @ Serial 214
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