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Author (up) De la Iglesia, R.; Valenzuela-Heredia, D.; Andrade, S.; Correa, J.; Gonzalez, B.
Title Composition dynamics of epilithic intertidal bacterial communities exposed to high copper levels Type
Year 2012 Publication Fems Microbiology Ecology Abbreviated Journal FEMS Microbiol. Ecol.
Volume 79 Issue 3 Pages 720-727
Keywords copA; copper; epilithic bacteria; intertidal rocky shore; T-RFLP
Abstract Copper has a dual role for organisms, both as micronutrient and toxic element. Copper mining activities have an enormous ecological impact because of the extraction process and the consequent release of copper-containing waste materials to the environment. In northern Chile, mainly in the Chanaral coastal area, this phenomenon is clearly evident. The released waste material has caused a strong modification of the area, and copper enrichment of beaches and rocky shores has provoked a decrease in the richness and diversity of many species of macroorganisms. However, the effects that copper enrichment has on microbial (e.g. bacterial epilithic) communities associated with the rocky shore environment are poorly understood. Using a culture-independent molecular approach, field sampling and laboratory microcosm experiments, we determined the effects of copper enrichment on bacterial communities inhabiting the rocky shore environment. Field samples showed a strong effect of copper on the structure of the natural bacterial epilithic communities, and microcosm experiments demonstrated rapid changes in bacterial community when copper is added, and reversibility of this effect within 48 h after copper is removed.
Address [Gonzalez, Bernardo] Univ Adolfo Ibanez, Fac Ingn & Ciencias, Santiago 7941169, Chile, Email: bernardo.gonzalez@uai.cl
Corporate Author Thesis
Publisher Wiley-Blackwell Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0168-6496 ISBN Medium
Area Expedition Conference
Notes WOS:000299257300014 Approved
Call Number UAI @ eduardo.moreno @ Serial 192
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Author (up) Morgante, V.; Lopez-Lopez, A.; Flores, C.; Gonzalez, M.; Gonzalez, B.; Vasquez, M.; Rossello-Mora, R.; Seeger, M.
Title Bioaugmentation with Pseudomonas sp strain MHP41 promotes simazine attenuation and bacterial community changes in agricultural soils Type
Year 2010 Publication Fems Microbiology Ecology Abbreviated Journal FEMS Microbiol. Ecol.
Volume 71 Issue 1 Pages 114-126
Keywords
Abstract Bioremediation is an important technology for the removal of persistent organic pollutants from the environment. Bioaugmentation with the encapsulated Pseudomonas sp. strain MHP41 of agricultural soils contaminated with the herbicide simazine was studied. The experiments were performed in microcosm trials using two soils: soil that had never been previously exposed to s-triazines (NS) and soil that had > 20 years of s-triazine application (AS). The efficiency of the bioremediation process was assessed by monitoring simazine removal by HPLC. The simazine-degrading microbiota was estimated using an indicator for respiration combined with most-probable-number enumeration. The soil bacterial community structures and the effect of bioaugmentation on these communities were determined using 16S RNA gene clone libraries and FISH analysis. Bioaugmentation with MHP41 cells enhanced simazine degradation and increased the number of simazine-degrading microorganisms in the two soils. In highly contaminated NS soil, bioaugmentation with strain MHP41 was essential for simazine removal. Comparative analysis of 16S rRNA gene clone libraries from NS and AS soils revealed high bacterial diversity. Bioaugmentation with strain MHP41 promoted soil bacterial community shifts. FISH analysis revealed that bioaugmentation increased the relative abundances of two phylogenetic groups (Acidobacteria and Planctomycetes) in both soils. Although members of the Archaea were metabolically active in these soils, their relative abundance was not altered by bioaugmentation.
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0168-6496 ISBN Medium
Area Expedition Conference
Notes WOS:000272452100013 Approved
Call Number UAI @ eduardo.moreno @ Serial 76
Permanent link to this record