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Author Timmermann, T.; Armijo, G.; Donoso, R.; Seguel, A.; Holuigue, L.; Gonzalez, B. pdf  doi
openurl 
  Title Paraburkholderia phytofirmans PsJN Protects Arabidopsis thaliana Against a Virulent Strain of Pseudomonas syringae Through the Activation of Induced Resistance Type
  Year 2017 Publication Molecular Plant-Microbe Interactions Abbreviated Journal Mol. Plant-Microbe Interact.  
  Volume 30 Issue 3 Pages 215-230  
  Keywords  
  Abstract Paraburkholderia phytofirmans PsJN is a plant growth promoting rhizobacterium (PGPR) that stimulates plant growth and improves tolerance to abiotic stresses. This study analyzed whether strain PsJN can reduce plant disease severity and proliferation of the virulent strain Pseudomonas syringae pv. tomato DC3000, in Arabidopsis plants, through the activation of induced resistance. Arabidopsis plants previously exposed to strain PsJN showed a reduction in disease severity and pathogen proliferation in leaves compared with noninoculated, infected plants. The plant defense related genes WRKY54, PR1, ERFI, and PDF1.2 demonstrated increased and more rapid expression in strain PsJN-treated plants compared with noninoculated, infected plants. Transcriptional analyses and functional analysis using signaling mutant plants suggested that resistance to infection by DC3000 in plants treated with strain PsJN involves salicylic acid, jasmonate-, and ethylene-signaling pathways to activate defense genes. Additionally, activation occurs through a specific PGPR-host recognition, being a necessary metabolically active state of the bacterium to trigger the resistance in Arabidopsis, with a strain PsJN associated molecular pattern only partially involved in the resistance response. This study provides the first report on the mechanism used by the PGPR P. phytofirmans PsJN to protect A. thaliana against a widespread virulent pathogenic bacterium.  
  Address [Timmermann, Tania; Donoso, Raul; Gonzalez, Bernardo] Univ Adolfo Ibanez, Fac Ingn & Ciencias, Lab Bioingn, Santiago, Chile, Email: bernardo.gonzalez@uai.cl  
  Corporate Author Thesis  
  Publisher Amer Phytopathological Soc Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0894-0282 ISBN Medium  
  Area Expedition Conference  
  Notes WOS:000399265100005 Approved  
  Call Number UAI @ eduardo.moreno @ Serial 720  
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Author Zuniga, A.; Donoso, R.A.; Ruiz, D.; Ruz, G.A.; Gonzalez, B. pdf  doi
openurl 
  Title Quorum-Sensing Systems in the Plant Growth-Promoting Bacterium Paraburkholderia phytofirmans PsJN Exhibit Cross-Regulation and Are Involved in Biofilm Formation Type
  Year 2017 Publication Molecular Plant-Microbe Interactions Abbreviated Journal Mol. Plant-Microbe Interact.  
  Volume 30 Issue 7 Pages 557-565  
  Keywords  
  Abstract Quorum-sensing systems play important roles in host colonization and host establishment of Burkholderiales species. Beneficial Paraburkholderia species share a conserved quorum-sensing (QS) system, designated BraI/R, that controls different phenotypes. In this context, the plant growth-promoting bacterium Paraburkholderia phytofirmans PsJN possesses two different homoserine lactone QS systems BpI. 1/R.1 and BpI. 2/R.2 (BraI/R-like QS system). The BpI. 1/R.1 QS system was previously reported to be important to colonize and produce beneficial effects in Arabidopsis thaliana plants. Here, we analyzed the temporal variations of the QS gene transcript levels in the wild-type strain colonizing plant roots. The gene expression patterns showed relevant differences in both QS systems compared with the wild-type strain in the unplanted control treatment. The gene expression data were used to reconstruct a regulatory network model of QS systems in P.phytofirmans PsJN, using a Boolean network model. Also, we examined the phenotypic traits and transcript levels of genes involved in QS systems, using P. phytofirmans mutants in homoserine lactone synthases genes. We observed that the BpI. 1/R.1 QS system regulates biofilm formation production in strain PsJN and this phenotype was associated with the lower expression of a specific extracytoplasmic function sigma factor ecf26.1 gene (implicated in biofilm formation) in the bpI.1 mutant strain.  
  Address [Gonzalez, Bernardo] Univ Adolfo Ibanez, Fac Ingn & Ciencias, Millennium Nucleus Ctr Plant Syst & Synthet Biol, Santiago, Chile, Email: bernardo.gonzalez@uai.cl  
  Corporate Author Thesis  
  Publisher Amer Phytopathological Soc Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0894-0282 ISBN Medium  
  Area Expedition Conference  
  Notes WOS:000404048400004 Approved  
  Call Number UAI @ eduardo.moreno @ Serial 739  
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Author Zuniga, A.; Poupin, M.J.; Donoso, R.; Ledger, T.; Guiliani, N.; Gutierrez, R.A.; Gonzalez, B. pdf  doi
openurl 
  Title Quorum Sensing and Indole-3-Acetic Acid Degradation Play a Role in Colonization and Plant Growth Promotion of Arabidopsis thaliana by Burkholderia phytofirmans PsJN Type
  Year 2013 Publication Molecular Plant-Microbe Interactions Abbreviated Journal Mol. Plant-Microbe Interact.  
  Volume 26 Issue 5 Pages 546-553  
  Keywords  
  Abstract Although not fully understood, molecular communication in the rhizosphere plays an important role regulating traits involved in plant-bacteria association. Burkholderia phytofirmans PsJN is a well-known plant-growth-promoting bacterium, which establishes rhizospheric and endophytic colonization in different plants. A competent colonization is essential for plant-growth-promoting effects produced by bacteria. Using appropriate mutant strains of B. phytofirmans, we obtained evidence for the importance of N-acyl homoserine lactone-mediated (quorum sensing) cell-to-cell communication in efficient colonization of Arabidopsis thaliana plants and the establishment of a beneficial interaction. We also observed that bacterial degradation of the auxin indole-3-acetic acid (IAA) plays a key role in plant-growth-promoting traits and is necessary for efficient rhizosphere colonization. Wildtype B. phytofirmans but not the iacC mutant in IAA mineralization is able to restore promotion effects in roots of A. thaliana in the presence of exogenously added IAA, indicating the importance of this trait for promoting primary root length. Using a transgenic A. thaliana line with suppressed auxin signaling (miR393) and analyzing the expression of auxin receptors in wild-type inoculated plants, we provide evidence that auxin signaling in plants is necessary for the growth promotion effects produced by B. phytofirmans. The interplay between ethylene and auxin signaling was also confirmed by the response of the plant to a 1-aminocyclopropane-1-carboxylate deaminase bacterial mutant strain.  
  Address Univ Adolfo Ibanez, Fac Ingn & Ciencias, Santiago, Chile, Email: bernardo.gonzalez@uai.cl  
  Corporate Author Thesis  
  Publisher Amer Phytopathological Soc Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0894-0282 ISBN Medium  
  Area Expedition Conference  
  Notes WOS:000317875400008 Approved  
  Call Number UAI @ eduardo.moreno @ Serial 277  
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