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Author Besaury, L.; Ouddane, B.; Pavissich, J.P.; Dubrulle-Brunaud, C.; Gonzalez, B.; Quillet, L. pdf  doi
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  Title Impact of copper on the abundance and diversity of sulfate-reducing prokaryotes in two chilean marine sediments Type
  Year 2012 Publication Marine Pollution Bulletin Abbreviated Journal Mar. Pollut. Bull.  
  Volume 64 Issue 10 Pages 2135-2145  
  Keywords Marine sediment; Copper contamination; Sulfate-reducing prokaryotes; Real-time PCR; DGGE; Phylogenetic analysis  
  Abstract We studied the abundance and diversity of the sulfate-reducing prokaryotes (SRPs) in two 30-cm marine chilean sediment cores, one with a long-term exposure to copper-mining residues, the other being a non-exposed reference sediment. The abundance of SRPs was quantified by qPCR of the dissimilatory sulfite reductase gene beta-subunit (dsrB) and showed that SRPs are sensitive to high copper concentrations, as the mean number of SRPs all along the contaminated sediment was two orders of magnitude lower than in the reference sediment. SRP diversity was analyzed by using the dsrB-sequences-based PCR-DGGE method and constructing gene libraries for dsrB-sequences. Surprisingly, the diversity was comparable in both sediments, with dsrB sequences belonging to Desulfobacteraceae, Syntrophobacteraceae, and Desulfobulbaceae, SRP families previously described in marine sediments, and to a deep branching dsrAB lineage. The hypothesis of the presence of horizontal transfer of copper resistance genes in the microbial population of the polluted sediment is discussed. (C) 2012 Elsevier Ltd. All rights reserved.  
  Address [Besaury, Ludovic; Quillet, Laurent] Univ Rouen, Lab Univ MEBE Microbiol Environm & Biol Evolut, F-76821 Mont St Aignan, France, Email: ludovic.besaury@etu.univ-rouen.fr;  
  Corporate Author Thesis  
  Publisher Pergamon-Elsevier Science Ltd Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0025-326x ISBN Medium  
  Area Expedition Conference  
  Notes WOS:000310929500032 Approved  
  Call Number UAI @ eduardo.moreno @ Serial (down) 254  
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