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Author (up) Torres, R.; Manriquez, P.H.; Duarte, C.; Navarro, J.M.; Lagos, N.A.; Vargas, C.A.; Lardies, M.A. pdf  url
  Title Evaluation of a semi-automatic system for long-term seawater carbonate chemistry manipulation Type
  Year 2013 Publication Revista Chilena De Historia Natural Abbreviated Journal Rev. Chil. Hist. Nat.  
  Volume 86 Issue 4 Pages 443-451  
  Keywords carbonate chemistry manipulation; ocean acidification  
  Abstract The assessment of the effects of Ocean Acidification (OA) on marine life has received increasing attention in recent marine research. On a mesocosmic scale, the CO2 levels in seawater can be manipulated to evaluate experimentally the consequences of OA on marine organisms (vertebrates and invertebrates). An ideal manipulation of carbonate chemistry should mimic exactly the changes to carbonate chemistry, which will occur in years to come. Although some methods have been described in the literature, here we describe in detail a simple, robust and inexpensive system to produce CO2-enriched seawater by bubbling the seawater with air-CO2 mixtures. The system uses mass flow controllers (MFC) to blend atmospheric air with pure CO2 to produce two pCO(2) levels. The air-CO2 mixtures are delivered continuously to seawater equilibration reservoirs, and simultaneously to an infrared CO2 analyser to verify CO2 levels in the air-CO2 mixture delivered to the equilibration tanks. We monitored both pH and total alkalinity in the equilibration reservoirs over a period of one year in order to document the long-term performance of this system for simulating the future carbonate chemistry of seawater in a coastal laboratory. System performance was sufficient to maintain three contrasting (e.g., 397, 709 and 1032 matm) and relatively constant (the coefficient of variability was 11 %, 9 % and 9 % respectively) seawater pCO(2) during a year-long monitoring.  
  Address [Torres, Rodrigo] CIEP, Coyhaique, Chile, Email:  
  Corporate Author Thesis  
  Publisher Soc Biolgia Chile Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0716-078x ISBN Medium  
  Area Expedition Conference  
  Notes WOS:000332750700006 Approved  
  Call Number UAI @ eduardo.moreno @ Serial 359  
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