toggle visibility Search & Display Options

Select All    Deselect All
 |   | 
Details
   print
  Record Links
Author (up) Meyer, L.A.; Johnson, M.G.; Cullen, D.M.; Vivanco, J.F.; Blank, R.D.; Ploeg, H.L.; Smith, E.L. pdf  doi
openurl 
  Title Combined exposure to big endothelin-1 and mechanical loading in bovine sternal cores promotes osteogenesis Type Journal Article
  Year 2016 Publication Bone Abbreviated Journal Bone  
  Volume 85 Issue Pages 115-122  
  Keywords Endothelin; Mechanical loading; Trabecular bone; Osteogenesis; Bioreactor  
  Abstract Increased bone formation resulting from mechanical loading is well documented; however, the interactions of the mechanotransduction pathways are less well understood. Endothelin-1, a ubiquitous autocrine/paracrine signaling molecule promotes osteogenesis in metastatic disease. In the present study, it was hypothesized that exposure to big endothelin-1 (big ET1) and/or mechanical loading would promote osteogenesis in ex vivo trabecular bone cores. In a 2 x 2 factorial trial of daily mechanical loading (-2000 μepsilon,120 cycles daily, “jump” waveform) and big ET1 (25 ng/mL), 48 bovine sternal trabecular bone cores were maintained in bioreactor chambers for 23 days. The bone cores' response to the treatment stimuli was assessed with percent change in core apparent elastic modulus (Delta E-app), static and dynamic histomorphometry, and prostaglandin E2 (PGE2) secretion. Two-way ANOVA with a post hoc Fisher's LSD test found no significant treatment effects on Delta E-app (p = 0.25 and 0.51 for load and big ET1, respectively). The Delta E-app in the “no load + big ET1” (CE, 13 +/- 12.2%, p = 0.56), “load + no big ET1” (LC, 17 +/- 3.9%, p = 0.14) and “load + big ETI” (LE, 19 +/- 4.2%, p = 0.13) treatment groups were not statistically different than the control group (CC, 3.3% +/- 8.6%). Mineralizing surface (MS/BS), mineral apposition (MAR) and bone formation rates (BFR/BS) were significantly greater in LE than CC (p = 0.037, 0.0040 and 0.019, respectively). While the histological bone formation markers in LC trended to be greater than CC (p = 0.055, 0.11 and 0.074, respectively) there was no difference between CE and CC (p = 0.61, 0.50 and 0.72, respectively). Cores in LE and LC had more than 50% greater MS/BS (p = 0.037, p = 0.055 respectively) and MAR (p = 0.0040, p = 0.11 respectively) than CC. The BFR/BS was more than two times greater in LE (p = 0.019) and LC (p = 0.074) than CC. The PGE2 levels were elevated at 8 days post-osteotomy in all groups and the treatment groups remained elevated compared to the CC group on days 15,19 and 23. The data suggest that combined exposure to big ET1 and mechanical loading results in increased osteogenesis as measured in biomechanical, histomorphometric and biochemical responses. (C) 2016 Elsevier Inc. All rights reserved.  
  Address [Meyer, Luisa A.; Vivanco, Juan F.; Ploeg, Heidi-Lynn] Univ Wisconsin, Dept Mech Engn, 1513 Univ Ave, Madison, WI 53706 USA, Email: lameyer2@wisc.edu;  
  Corporate Author Thesis  
  Publisher Elsevier Science Inc Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 8756-3282 ISBN Medium  
  Area Expedition Conference  
  Notes WOS:000372770200015 Approved no  
  Call Number UAI @ eduardo.moreno @ Serial 606  
Permanent link to this record
Select All    Deselect All
 |   | 
Details
   print

Save Citations:
Export Records: