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Author (up) Loyola, R.; Herrera, D.; Mas, A.; Wong, D.C.J.; Holl, J.; Cavallini, E.; Amato, A.; Azuma, A.; Ziegler, T.; Aquea, F.; Castellarin, S.D.; Bogs, J.; Tornielli, G.B.; Pena-Neira, A.; Czemmel, S.; Alcalde, J.A.; Matus, J.T.; Arce-Johnson, P. pdf  doi
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  Title The photomorphogenic factors UV-B RECEPTOR 1, ELONGATED HYPOCOTYL 5, and HY5 HOMOLOGUE are part of the UV-B signalling pathway in grapevine and mediate flavonol accumulation in response to the environment Type
  Year 2016 Publication Journal Of Experimental Botany Abbreviated Journal J. Exp. Bot.  
  Volume 67 Issue 18 Pages 5429-5445  
  Keywords Binding; glycosyltransferase; MYBF1; network; photolyase; ripening; UVR8  
  Abstract By performing molecular studies coupled to radiation experiments and in silico systems analyses, we have ascertained the role of the grapevine UV-B receptor and two HY5 homologues in regulating flavonol synthesis.Grapevine (Vitis vinifera L.) is a species well known for its adaptation to radiation. However, photomorphogenic factors related to UV-B responses have not been molecularly characterized. We cloned and studied the role of UV-B RECEPTOR (UVR1), ELONGATED HYPOCOTYL 5 (HY5), and HY5 HOMOLOGUE (HYH) from V. vinifera. We performed gene functional characterizations, generated co-expression networks, and tested them in different environmental conditions. These genes complemented the Arabidopsis uvr8 and hy5 mutants in morphological and secondary metabolic responses to radiation. We combined microarray and RNA sequencing (RNA-seq) data with promoter inspections to identify HY5 and HYH putative target genes and their DNA binding preferences. Despite sharing a large set of common co-expressed genes, we found different hierarchies for HY5 and HYH depending on the organ and stress condition, reflecting both co-operative and partially redundant roles. New candidate UV-B gene markers were supported by the presence of HY5-binding sites. These included a set of flavonol-related genes that were up-regulated in a HY5 transient expression assay. We irradiated in vitro plantlets and fruits from old potted vines with high and low UV-B exposures and followed the accumulation of flavonols and changes in gene expression in comparison with non-irradiated conditions. UVR1, HY5, and HYH expression varied with organ, developmental stage, and type of radiation. Surprisingly, UVR1 expression was modulated by shading and temperature in berries, but not by UV-B radiation. We propose that the UV-B response machinery favours berry flavonol accumulation through the activation of HY5 and HYH at different developmental stages at both high and low UV-B exposures.  
  Address [Loyola, Rodrigo; Herrera, Daniela; Arce-Johnson, Patricio] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Genet Mol & Microbiol, Alameda 340, Santiago, Chile, Email: jalcalde@uc.cl;  
  Corporate Author Thesis  
  Publisher Oxford Univ Press Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0022-0957 ISBN Medium  
  Area Expedition Conference  
  Notes WOS:000386067000014 Approved  
  Call Number UAI @ eduardo.moreno @ Serial 667  
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