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Author (up) Pavissich, J.P.; Silva, M.; Gonzalez, B. pdf  doi
  Title Sulfate Reduction, Molecular Diversity, And Copper Amendment Effects In Bacterial Communities Enriched From Sediments Exposed To Copper Mining Residues Type
  Year 2010 Publication Environmental Toxicology And Chemistry Abbreviated Journal Environ. Toxicol. Chem.  
  Volume 29 Issue 2 Pages 256-264  
  Keywords Sulfate reduction; Coastal sediments; Sulfate-reducing bacteria; Copper; Molecular diversity  
  Abstract Sulfate-reducing bacterial communities from coastal sediments with a long-term exposure to copper (Cu)-mining residues were studied in lactate enrichments. The toxicity of excess copper may affect sulfate-reducing bacterial communities. Sulfate reduction was monitored by sulfate and organic acid measurements. Molecular diversity was analyzed by 16S rRNA, dissimilatory sulfate reduction dsrAB, and Cu translocating phospho-type adenosine triphosphatases (P-ATPases) cop-like gene sequence profiling. The influence of Cu amendment was tested in these enrichments. Results showed fast sulfate reduction mostly coupled to incomplete organic carbon oxidation and partial sulfate reduction inhibition due to copper amendment. The 16S rRNA clonal libraries analysis indicated that delta- and gamma-Proteobacteria and Cytophaga-Flexibacter-Bacteroides dominated the enrichments. The dsrAB libraries revealed the presence of Desulfobacteraceae and Desulfovibrionaceae families-related sequences. Copper produced significant shifts (i.e., a decrease in the relative abundance of sulfate-reducing microorganisms) in the enriched bacterial community structure as determined by terminal-restriction fragment length polymorphism (T-RFLP) profiling and multivariate analyses. Clonal libraries of cop-like sequences showed low richness in the enriched microbial communities, and a strong effect of copper on its relative abundance. Novel Cu-P-IB-ATPase sequences encoding Cu resistance were detected. The present study indicates that Cu does not significantly affect sulfate reduction and genetic diversity of taxonomic and dissimilatory sulfate-reduction molecular markers. However, the diversity of Cu resistance genetic determinants was strongly modified by this toxic metal. Environ. Toxicol. Chem. 2010;29:256-264. (C) 2009 SETAC  
  Address [Pavissich, Juan P.; Silva, Macarena; Gonzalez, Bernardo] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Millennium Nucleus Microbial Ecol & Environm Micr, Dept Mol Genet & Microbiol,CASEB, Santiago, Chile, Email:  
  Corporate Author Thesis  
  Publisher Setac Press Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0730-7268 ISBN Medium  
  Area Expedition Conference  
  Notes WOS:000274272500004 Approved  
  Call Number UAI @ eduardo.moreno @ Serial 81  
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