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Author (up) Mancilla, R.A.; Little, C.; Amoroso, A. pdf  doi
  Title Efficient Bioconversion of High Concentration Phytosterol Microdispersion to 4-Androstene-3,17-Dione (AD) by Mycobacterium sp B3805 Type Journal Article
  Year 2018 Publication Applied Biochemistry And Biotechnology Abbreviated Journal Appl. Biochem. Biotechnol.  
  Volume 185 Issue 2 Pages 494-506  
  Keywords Bioconversion; Phytosterols; Microdispersion; AD; Steroids  
  Abstract Low solubility of sterols in aqueous media limits efficient steroid production mediated by biocatalytic microorganisms such as Mycobacterium. Sterol emulsion technologies have been developed with low success rates, largely due to the complexity of generating stable and bioavailable particles. In this study, several aqueous dispersions of sterols in-water of different particle sizes were bioconverted to 4-androstene-3,17-dione (AD) in a solvent-free environment, using a classic microorganism Mycobacterium sp. B3805 as a model system. According to our results, the high concentration (20 g/L) phytosterol dispersions with the smallest particle size tested (370 nm) achieved up to 54% (7.4 g/L) AD production yield in 11 days. Moreover, the use of 0.1 biomass/sterols ratio in a complex bioconversion media containing yeast extract, and a 1:1 glucose/microdispersion ratio in the presence of the surfactant DK-Ester P-160 (HLB16), allowed homogenization and increased microdispersion stability, thus achieving the best results using emulsion technologies to date.  
  Address [Mancilla, Rodrigo A.] Pontificia Univ Catolica Valparaiso, Fac Biochem Engn, Valparaiso 2340000, Chile, Email:  
  Corporate Author Thesis  
  Publisher Humana Press Inc Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0273-2289 ISBN Medium  
  Area Expedition Conference  
  Notes WOS:000431503400011 Approved no  
  Call Number UAI @ eduardo.moreno @ Serial 849  
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