toggle visibility Search & Display Options

Select All    Deselect All
 |   | 
Details
   print
  Record Links
Author (up) Romero-Romero, J.L.; Inostroza-Blancheteau, C.; Orellana, D.; Aquea, F.; Reyes-Diaz, M.; Gil, P.M.; Matte, J.P.; Arce-Johnson, P. doi  openurl
  Title Stomata regulation by tissue-specific expression of the Citrus sinensis MYB61 transcription factor improves water-use efficiency in Arabidopsis Type Journal Article
  Year 2018 Publication Plant Physiology And Biochemistry Abbreviated Journal Plant Physiol. Biochem.  
  Volume 130 Issue Pages 54-60  
  Keywords Drought tolerance; Abiotic stress; Water use efficiency; Arabidopsis; Cisgenic; MYB61 transcription factor; Citrus  
  Abstract Water-use efficiency (WUE) is a quantitative measurement of biomass produced per volume of water transpired by a plant. WUE is an important physiological trait for drought response to mitigate the water deficiency. In this work, a cisgenic construction from Citrus sinensis was developed and its function in the improvement of WUE was evaluated in Arabidopsis. Sequences of the CsMYB61 coding region, a transcription factor implicated in the closure of stomata, together with a putative stomata-specific promoter from CsMYB.1.5, were identified and cloned. The protein encoded in the CsMYB61 locus harbors domains and motifs characteristic of MYB61 proteins. In addition, a 1.2 kb promoter region of the gene CsMYB15 (pCsMYB15) containing regulatory elements for expression in guard cells and in response to Abscisic Acid (ABA) and light was isolated. In Arabidopsis, pCsMYB15 directs the expression of the reporter gene GUS in stomata in the presence of light. In addition, transgenic lines expressing the CsMYB61 coding region under transcriptional control of pCsMYB15 have a normal phenotype under in vitro and greenhouse conditions. These transgenic lines exhibited a smaller opening of the stomata pore, lower stomatal conductance and respiration rate, enhanced sensitivity to exogenous ABA, and high drought stress tolerance. Our results indicate that stomata-specific expression of CsMYB61 enhances water use efficiency under drought conditions in Arabidospis.  
  Address [Romero-Romero, Jesus L.; Orellana, Daniela; Pablo Matte, Juan; Arce-Johnson, Patricio] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Genet Mol & Microbiol, Santiago, Chile, Email: parce@bio.puc.cl  
  Corporate Author Thesis  
  Publisher Elsevier France-Editions Scientifiques Medicales Elsevier Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0981-9428 ISBN Medium  
  Area Expedition Conference  
  Notes WOS:000444789200006 Approved no  
  Call Number UAI @ eduardo.moreno @ Serial 915  
Permanent link to this record
Select All    Deselect All
 |   | 
Details
   print

Save Citations:
Export Records: