Argiz, L., Reyes, C., Belmonte, M., Franchi, O., Campo, R., Fra-Vazquez, A., et al. (2020). Assessment of a fast method to predict the biochemical methane potential based on biodegradable COD obtained by fractionation respirometric tests. J. Environ. Manage., 269, 9 pp.
Abstract: The biochemical methane potential test (BMP) is the most common analytical technique to predict the performance of anaerobic digesters. However, this assay is time-consuming (from 20 to over than 100 days) and consequently impractical when it is necessary to obtain a quick result. Several methods are available for faster BMP prediction but, unfortunately, there is still a lack of a clear alternative. Current aerobic tests underestimate the BMP of substrates since they only detect the easily biodegradable COD. In this context, the potential of COD fractionation respirometric assays, which allow the determination of the particulate slowly biodegradable fraction, was evaluated here as an alternative to early predict the BMP of substrates. Seven different origin waste streams were tested and the anaerobically biodegraded organic matter (CODmet) was compared with the different COD fractions. When considering adapted microorganisms, the appropriate operational conditions and the required biodegradation time, the differences between the CODmet, determined through BMP tests, and the biodegradable COD (CODb) obtained by respirometry, were not significant (CODmet (57.8026 +/- 21.2875) and CODb (55.6491 +/- 21.3417), t (5) = 0.189, p = 0.853). Therefore, results suggest that the BMP of a substrate might be early predicted from its CODb in only few hours. This methodology was validated by the performance of an inter-laboratory studyconsidering four additional substrates.
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Belmonte, M., Hsieh, C. F., Campos, J. L., Guerrero, L., Mendez, R., Mosquera-Corral, A., et al. (2017). Effect of Free Ammonia, Free Nitrous Acid, and Alkalinity on the Partial Nitrification of Pretreated Pig Slurry, Using an Alternating Oxic/Anoxic SBR. Biomed Res. Int., , 7 pp.
Abstract: The effect of free ammonia (NH3 or FA), free nitrous acid (HNO2 or FNA), and total alkalinity (TA) on the performance of a partial nitrification (PN) sequencing batch reactor (SBR) treating anaerobically pretreated pig slurry was studied. The SBR was operated under alternating oxic/anoxic (O/A) conditions and was fed during anoxic phases. This strategy allowed using organic matter to partially remove nitrite (NO2-) andnitrate (NO3-) generated during oxic phases. The desired NH4+ to NO2- ratioof 1.3gN/gNwas obtained when an Ammonium Loading Rate (ALR) of 0.09 gNH(4)(+)N/L d was applied. The system was operated at a solid retention time (SRT) of 15-20 d and dissolved oxygen (DO) levels higher than 3 mg O-2/L during the whole operational period. PN mainly occurred caused by the inhibitory effect of FNA on nitrite oxidizing bacteria (NOB). Once HNO2 concentration was negligible, NH4+ was fully oxidized to NO(3)(-)in spite of the presence of FA. The use of biomass acclimated to ammonium as inoculum avoided a possible effect of FA on NOB activity.
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Campos, J. L., Crutchik, D., Franchi, O., Pavissich, J. P., Belmonte, M., Pedrouso, A., et al. (2019). Nitrogen and Phosphorus Recovery From Anaerobically Pretreated Agro-Food Wastes: A Review. Front. Sustain. Food Syst., 2, 11 pp.
Abstract: Anaerobic digestion (AD) is commonly used for the stabilization of agro-food wastes and recovery of energy as methane. Since AD removes organic C but not nutrients (N and P), additional processes to remove them are usually applied to meet the stringent effluent criteria. However, in the past years, there was a shift from the removal to the recovery of nutrients as a result of increasing concerns regarding limited natural resources and the importance given to the sustainable treatment technologies. Recovering N and P from anaerobically pretreated agro-food wastes as easily transportable and marketable products has gained increasing importance to meet both regulatory requirements and increase revenue. For this reason, this review paper gives a critical comparison of the available and emerging technologies for N and P recovery from AD residues.
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Campos, J. L., Dumais, J., Pavissich, J. P., Franchi, O., Crutchik, D., Belmonte, M., et al. (2019). Predicting Accumulation of Intermediate Compounds in Nitrification and Autotrophic Denitrification Processes: A Chemical Approach. Biomed Res. Int., 2019, 9 pp.
Abstract: Nitrification and sulfur-based autotrophic denitrification processes can be used to remove ammonia from wastewater in an economical way. However, under certain operational conditions, these processes accumulate intermediate compounds, such as elemental sulphur, nitrite, and nitrous oxide, that are noxious for the environment. In order to predict the generation of these compounds, an analysis based on the Gibbs free energy of the possible reactions and on the oxidative capacity of the bulk liquid was done on case study systems. Results indicate that the Gibbs free energy is not a useful parameter to predict the generation of intermediate products in nitrification and autotrophic denitrification processes. Nevertheless, we show that the specific productions of nitrous oxide during nitrification, and of elemental sulphur and nitrite during autotrophic denitrification, are well related to the oxidative capacity of the bulk liquid.
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Campos, J. L., Valenzuela-Heredia, D., Pedrouso, A., del Rio, A. V., Belmonte, M., & Mosquera-Corral, A. (2016). Greenhouse Gases Emissions from Wastewater Treatment Plants: Minimization, Treatment, and Prevention. J. Chem., 3796352, 12 pp.
Abstract: The operation of wastewater treatment plants results in direct emissions, from the biological processes, of greenhouse gases (GHG) such as carbon dioxide (CO2), methane (CH4), and nitrous oxide (N-2 O), as well as indirect emissions resulting from energy generation. In this study, three possible ways to reduce these emissions are discussed and analyzed: (1) minimization through the change of operational conditions, (2) treatment of the gaseous streams, and (3) prevention by applying new configurations and processes to remove both organic matter and pollutants. In current WWTPs, to modify the operational conditions of existing units reveals itself as possibly the most economical way to decrease N-2 O and CO2 emissions without deterioration of effluent quality. Nowadays the treatment of the gaseous streams containing the GHG seems to be a not suitable option due to the high capital costs of systems involved to capture and clean them. The change of WWTP configuration by using microalgae or partial nitritation-Anammox processes to remove ammonia from wastewater, instead of conventional nitrification-denitrification processes, can significantly reduce the GHG emissions and the energy consumed. However, the area required in the case of microalgae systems and the current lack of information about stability of partial nitritation-Anammox processes operating in the main stream of the WWTP are factors to be considered.
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Cofre, C., Campos, J. L., Valenzuela-Heredia, D., Pavissich, J. P., Camus, N., Belmonte, M., et al. (2018). Novel system configuration with activated sludge like-geometry to develop aerobic granular biomass under continuous flow. Bioresour. Technol., 267, 778–781.
Abstract: A novel continuous flow system with “flat geometry” composed by two completely mixed aerobic tanks in series and a settler was used to promote the formation of aerobic granular sludge. Making similarities of this system with a typical sequencing batch reactor (SBR), for aerobic granules cultivation, the value of the tank 1/tank 2 vol ratio and the biomass recirculation rate would correspond with the feast/famine length ratio and the length of the operational cycle, respectively, while the settler upflow liquid velocity imposed would be related to the settling time. From the three experiments performed the best results were obtained when the tank 1/tank 2 vol ratio was of 0.28, the sludge recycling ratio of 0.25 and the settler upflow velocity of 2.5 m/h. At these conditions the aggregates had settling velocities between 29 and 113 m/h, sludge volume index at 10 min (SVI10) of 70 mL/g TSS and diameters between 1.0 and 5.0 mm.
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Crutchik, D., Franchi, O., Caminos, L., Jeison, D., Belmonte, M., Pedrouso, A., et al. (2020). Polyhydroxyalkanoates (PHAs) Production: A Feasible Economic Option for the Treatment of Sewage Sludge in Municipal Wastewater Treatment Plants? Water, 12(4), 12 pp.
Abstract: Sludge is a by-product of municipal wastewater treatment plants (WWTPs) and its management contributes significantly to the operating costs. Large WWTPs usually have anaerobic sludge digesters to valorize sludge as methane and to reduce its mass. However, the low methane market price opens the possibility for generating other high value-added products from the organic matter in sludge, such as polyhydroxyalkanoates (PHAs). In this work, the economic feasibility of retrofitting two types of WWTPs to convert them into biofactories of crude PHAs was studied. Two cases were analyzed: (a) a large WWTP with anaerobic sludge digestion; and (b) a small WWTP where sludge is only dewatered. In a two-stage PHA-production system (biomass enrichment plus PHAs accumulation), the minimum PHAs cost would be 1.26 and 2.26 US$/kg PHA-crude for the large and small WWTPs, respectively. In a single-stage process, where a fraction of the secondary sludge (25%) is directly used to accumulate PHAs, the production costs would decrease by around 15.9% (small WWTPs) and 19.0% (large WWTPs), since capital costs associated with bioreactors decrease. Sensitivity analysis showed that the PHA/COD (Chemical Oxygen Demand) yield is the most crucial parameter affecting the production costs. The energy, methane, and sludge management prices also have an essential effect on the production costs, and their effect depends on the WWTP's size.
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Diaz, C., Belmonte, M., Campos, J. L., Franchi, O., Faundez, M., Vidal, G., et al. (2020). Limits of the anammox process in granular systems to remove nitrogen at low temperature and nitrogen concentration. Process Saf. Environ. Protect., 138, 349–355.
Abstract: When partial nitritation-anammox (PN-AMX) processes are applied to treat the mainstream in wastewater treatment plants (WWTPs), it is difficult to fulfil the total nitrogen (TN) quality requirements established by the European Union (<10g TN/m(3)). The operation of the anammox process was evaluated here in a continuous stirred tank reactor operated at 15 degrees C and fed with concentrations of 50 g TN/m(3) (1.30 +/- 0.23 g NO2- -N/g NH4+-N). Two different aspects were identified as crucial, limiting nitrogen removal efficiency. On the one hand, the oxygen transferred from the air in contact with the mixed liquor surface favoured the nitrite oxidation to nitrate (up to 75 %) and this nitrate, in addition to the amount produced from the anammox reaction itself, worsened the effluent quality. On the other hand, the mass transfer of ammonium and nitrite to be converted inside the anammox granules involves relatively large values of apparent affinity constants (k(NH4+app) : 0.50 g NH4+-N/m(3) ; k(NO2-app) 0.17 g NO2--N/m(3)) that favour the presence of these nitrogen compounds in the produced effluent. The careful isolation of the reactor from air seeping and the fixation of right hydraulic and solids retention times are expected to help the maintenance of stability and effluent quality. (C) 2020 Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.
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Franchi, O., Alvarez, M. I., Pavissich, J. P., Belmonte, M., Pedrouso, A., del Rio, A. V., et al. (2024). Operational variables and microbial community dynamics affect granulation stability in continuous flow aerobic granular sludge reactors. J. Water Process Eng., 59, 104951.
Abstract: Retrofitting wastewater treatment plants with continuous aerobic granular sludge reactors is a promising alternative to enhance treatment capacities and reduce footprint. This study investigates the main variables influencing granulation and microbial dynamics in two reactor configurations (25 L): stirred tanks in series (R1) and a plug-flow-like system (R2). Granule formation was achieved by increasing the organic loading rate (OLR) from 0.7 to 4.1 kg COD/(m3 & sdot;d) and the up-flow velocity in the biomass selector from 1.4 to 6.9 m/h. However, irreversible granule destabilization occurred at day 68 for R1 and day 108 for R2. Principal component analysis and examination of food-to-microorganisms (F/M) ratio medians identified the F/M ratio as the primary variable associated with instability. Microbial analysis revealed that a high F/M ratio induced significant increases in the abundance of specific genera such as Arcobacter, Cloacibacterium, Rikenella, Aquaspirillum and Sphaerotillus, whose overgrowth may negatively impact granule stability. Based on these findings, maximum F/M ratio thresholds were obtained to establish operational conditions allowing the maintenance of stable aerobic granules on continuous flow reactor configurations.
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Ortega, V., Donoso-Bravo, A., Chamy-Maggy, R., Campos, J. L., Mosquera-Corral, A., & Belmonte, M. (2022). Factors That Affect Methane Yield Using Raw Olive Alperujo (Unhydrolyzed) as Substrate in BMP Assays. Recycling, 7(2), 15.
Abstract: The olive alperujo (OA) corresponds to the solid waste generated in the olive oil extraction process using the two-phase centrifugation method. OA is produced in large quantities (800 kg OA/ton olives processed) and is characterized by its high moisture content, organic matter, and low pH. In Chile, the olive oil industry is recent, and one of its main challenges is to be able to manage OA to reduce the impact caused by its disposal. In this sense, its valorization as biogas by means of anaerobic digestion is an economically attractive option. For this, it is previously necessary to evaluate the biomethane potential (BMP) of the raw OA using batch assays. This study was focused on evaluating the factors that most affect the methane yield (MY) when using OA as substrate in BMP tests. First, a sweep analysis (Plackett-Burman) was applied to determine those factors that, according to the literature, would have an influence on the BMP tests. Among the factors studied, the most significant were preincubation, OA concentration, and agitation level. Subsequently, a 2(3) factorial experimental design was applied to evaluate the effect of these factors on MY at different levels. Results show that the OA concentration was the most significant factor affecting MY.
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Valenzuela-Heredia, D., Panatt, C.:, Belmonte, M., Franchi, O., Crutchik, D., Dumais, J., Vazquez-Padin, J. R., et al. (2022). Performance of a two-stage partial nitritation-anammox system treating the supernatant of a sludge anaerobic digester pretreated by a thermal hydrolysis process. Chem. Eng. J., 429, 131301.
Abstract: A two-stage system (partial nitritation (PN) and anammox processes) was used to remove nitrogen from the dewatering liquor originating from the thermal hydrolysis/anaerobic digestion (THP/AD) of municipal WWTP sludge. Two strategies were tested to start up the PN reactor: 1) maintaining a fixed hydraulic retention time (HRT) and increasing the ammonium loading rate (ALR) by decreasing the feeding dilution ratio and 2) feeding undiluted dewatering liquor and gradually decreasing the HRT. With diluted feeding, the reactor performance had destabilization episodes that were statistically correlated with the application of high specific ammonium (> 0.6 g NH4+-N/(g TSS.d)) and organic (> 0.7 g COD/(g TSS.d)) loading rates. The second strategy allowed stable PN reactor operation while treating ALR up to 4.8 g NH4+-N/(L.d) and demonstrating that dilution of THP/AD effluents is not required. The operating conditions promoted the presence of free nitrous acid levels (> 0.14 mg HNO2-N/L) inside the PN reactor that inhibited the proliferation of nitrite oxidizing bacteria.
Batch activity tests showed that the inhibitory effects of organic compounds present in the THP/AD dewatering liquor on the ammonia oxidizing bacteria activity can be removed in the PN reactor. Thus, aerobic pretreatment would not be necessary when two-stage systems are used. The PN reactor effluent was successfully treated by an anammox reactor.
An economic analysis showed that using two-stage systems is advantageous for treating THP/AD dewatering liquor. The implementation of an aerobic pre-treatment unit is recommended for WWTPs capacities higher than 5.10(5) inhabitants equivalent when one-stage systems are used.
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