Abstract: Molecularly imprinted polymer nanoparticles (nanoMIPs) are receiving broad interest as robust and highly selective synthetic receptors for a variety of molecules. Due to their stability, inexpensive synthesis and easy implementation, they are considered a promising alternative to antibodies in sensors, diagnostics and separation applications. The most challenging targets for the production of synthetic receptors are proteins due to their fragile nature and the multitude of possible binding sites in their structure. Herein, we describe the modification and optimization of the protocol for synthesis of nanoMIPs with specificity for proteins using the prototype of an automated solid-phase synthesizer. Using an automated system gives an advantage for the simple, fast and fully controlled, reproducible production of nanoMIPs. The molecular imprinting in the reactor is performed using a template covalently immobilized on a solid support, in mild conditions suitable for preserving protein native structure. The validation of the protocol was made by assessing the ability to regenerate a solid-phase, and by measuring affinity and specificity of nanoparticles. As a model protein, we have chosen trypsin since its enzymatic activity can be easily monitored by using a commercial colorimetric assay. Different protocols were tested for their ability to improve the yield of high affinity nanoparticles in the final elution.

Abstract: The White Dwarf Binary Pathways Survey aims at increasing the number of known detached A, F, G, and K main-sequence stars in close orbits with white dwarf companions (WD+AFGK binaries) to refine our understanding about compact binary evolution and the nature of Supernova Ia progenitors. These close WD+AFGK binary stars are expected to form through common envelope evolution, in which tidal forces tend to circularize the orbit. However, some of the identified WD+AFGK binary candidates show eccentric orbits, indicating that these systems are either formed through a different mechanism or perhaps they are not close WD+AFGK binaries. We observed one of these eccentric WD+AFGK binaries with SPHERE and find that the system TYC 7218-934-1 is in fact a triple system where the WD is a distant companion. The inner binary likely consists of the G-type star plus an unseen low-mass companion in an eccentric orbit. Based on this finding, we estimate the fraction of triple systems that could contaminate the WD+AFGK sample. We find that less than 15 per cent of our targets with orbital periods shorter than 100 d might be hierarchical triples.

Abstract: In underground mining, the design of rock pillars is of crucial importance as these are structures that allow safe mining by maintaining the stability of the surrounding excavations. Pillar design is often a complex task, as it involves estimating the loads at depths and the strength of the rock mass fabric, which depend on the intact strength of the rock and the shape of the pillar in terms of the aspect ratio (width/height). The design also depends on the number, persistence, orientation, and strength of the discontinuities with respect to the orientation and magnitude of the stresses present. Solutions to this engineering problem are based on one or more of the following approaches: empirical design methods, practical experience, and/or numerical modeling. Based on the similarities between masonry structures and rock mass characteristics, an equivalent approach is proposed as the one commonly used in masonry but applied to rock pillar design. Numerical models using different geometric configurations and state of stresses are carried out using a finite difference numerical approach with an adapted masonry model applied to rocks. The results show the capability of the numerical approach to replicate common types of pillar failure modes and stability thresholds as those observed in practice.