Viveros, M. F. A., Inostroza-Blancheteau, C., Timmermann, T., Gonzalez, M., & Arce-Johnson, P. (2013). Overexpression of GlyI and GlyII genes in transgenic tomato (Solanum lycopersicum Mill.) plants confers salt tolerance by decreasing oxidative stress. Mol. Biol. Rep., 40(4), 3281–3290.
Abstract: The glyoxalase system plays an important role in various physiological processes in plants, including salt stress tolerance. We report the effects of overexpressing glyoxalase I and glyoxalase II genes in transgenic tomato (Solanum lycopersicum Mill.) cv. Ailsa Craig. Stable expression of both transgenes was detected in the transformed tomato plants under salt stress. The transgenic lines overexpressing GlyI and GlyII under a high NaCl concentration (800 mM) showed reduced lipid peroxidation and the production of H2O2 in leaf tissues. A greater decrease in the chlorophyll a+b content in wild-type (WT) compared with transgenic lines was also observed. These results suggest that the over expression of two genes, GlyI and GlyII, may enhance salt stress tolerance by decreasing oxidative stress in transformed tomato plants. This work will help our understanding of the putative role of the glyoxalase system in the tolerance to abiotic stress in tomato plants.
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Morgante, V., Lopez-Lopez, A., Flores, C., Gonzalez, M., Gonzalez, B., Vasquez, M., et al. (2010). Bioaugmentation with Pseudomonas sp strain MHP41 promotes simazine attenuation and bacterial community changes in agricultural soils. FEMS Microbiol. Ecol., 71(1), 114–126.
Abstract: Bioremediation is an important technology for the removal of persistent organic pollutants from the environment. Bioaugmentation with the encapsulated Pseudomonas sp. strain MHP41 of agricultural soils contaminated with the herbicide simazine was studied. The experiments were performed in microcosm trials using two soils: soil that had never been previously exposed to s-triazines (NS) and soil that had > 20 years of s-triazine application (AS). The efficiency of the bioremediation process was assessed by monitoring simazine removal by HPLC. The simazine-degrading microbiota was estimated using an indicator for respiration combined with most-probable-number enumeration. The soil bacterial community structures and the effect of bioaugmentation on these communities were determined using 16S RNA gene clone libraries and FISH analysis. Bioaugmentation with MHP41 cells enhanced simazine degradation and increased the number of simazine-degrading microorganisms in the two soils. In highly contaminated NS soil, bioaugmentation with strain MHP41 was essential for simazine removal. Comparative analysis of 16S rRNA gene clone libraries from NS and AS soils revealed high bacterial diversity. Bioaugmentation with strain MHP41 promoted soil bacterial community shifts. FISH analysis revealed that bioaugmentation increased the relative abundances of two phylogenetic groups (Acidobacteria and Planctomycetes) in both soils. Although members of the Archaea were metabolically active in these soils, their relative abundance was not altered by bioaugmentation.
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Miranda, A., Mentler, R., Moletto-Lobos, I., Alfaro, G., Aliaga, L., Balbontin, D., et al. (2022). The Landscape Fire Scars Database: mapping historical burned area and fire severity in Chile. Earth Syst. Sci. Data, 14(8), 3599–3613.
Abstract: Achieving a local understanding of fire regimes requires high-resolution, systematic and dynamic databases. High-quality information can help to transform evidence into decision-making in the context of rapidly changing landscapes, particularly considering that geographical and temporal patterns of fire regimes and their trends vary locally over time. Global fire scar products at low spatial resolutions are available, but high-resolution wildfire data, especially for developing countries, are still lacking. Taking advantage of the Google Earth Engine (GEE) big-data analysis platform, we developed a flexible workflow to reconstruct individual burned areas and derive fire severity estimates for all reported fires. We tested our approach for historical wild-fires in Chile. The result is the Landscape Fire Scars Database, a detailed and dynamic database that reconstructs 8153 fires scars, representing 66.6% of the country's officially recorded fires between 1985 and 2018. For each fire event, the database contains the following information: (i) the Landsat mosaic of pre- and post-fire images; (ii) the fire scar in binary format; (iii) the remotely sensed estimated fire indexes (the normalized burned ratio, NBR, and the relative delta normalized burn ratio, RdNBR); and two vector files indicating (iv) the fire scar perimeter and (v) the fire scar severity reclassification, respectively. The Landscape Fire Scars Database for Chile and GEE script (JavaScript) are publicly available. The framework developed for the database can be applied anywhere in the world, with the only requirement being its adaptation to local factors such as data availability, fire regimes, land cover or land cover dynamics, vegetation recovery, and cloud cover. The Landscape Fire Scars Database for Chile is publicly available in https://doi.org/10.1594/PANGAEA.941127 (Miranda et al., 2022).
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Gonzalez, M. E., Galleguillos, M., Lopatin, J., Leal, C., Becerra-Rodas, C., Lara, A., et al. (2022). Surviving in a hostile landscape: Nothofagus alessandrii remnant forests threatened by mega-fires and exotic pine invasion in the coastal range of central Chile. Oryx, Early Access.
Abstract: Nothofagus alessandrii, categorized as Endangered on the IUCN Red List, is an endemic, deciduous tree species of the coastal range of central Chile. We assessed the effects of fire severity, invasion by the exotic fire-prone Pinus radiata, and land-cover composition and configuration of the landscape on the resilience of fragments of N. alessandrii after a mega-fire in 2017. We used remote sensing data to estimate land-use classes and cover, fire severity and invasion cover of P. radiata. We monitored forest composition and structure and post-fire responses of N. alessandrii forests in situ for 2 years after the mega-fire. In the coastal Maule region wildfires have been favoured by intense drought and widespread exotic pine plantations, increasing the ability of fire-adapted invasive species to colonize native forest remnants. Over 85% of N. alessandrii forests were moderately or severely burnt. The propagation and severity of fire was probably amplified by the exotic pines located along the edges of, or inside, the N. alessandrii fragments and the highly flammable pine plantations surrounding these fragments (> 60% of land use is pine plantations). Pinus radiata, a fire-adapted pioneer species, showed strong post-fire recruitment within the N. alessandrii fragments, especially those severely burnt. Positive feedback between climate change (i.e. droughts and heat waves), wildfires and pine invasions is driving N. alessandrii forests into an undesirable and probably irreversible state (i.e. a landscape trap). A large-scale restoration programme to design a diverse and less flammable landscape is needed to avoid the loss of these highly threatened forest ecosystems.
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