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Lavin, P., Gonzalez, B., Santibanez, J. F., Scanlan, D. J., & Ulloa, O. (2010). Novel lineages of Prochlorococcus thrive within the oxygen minimum zone of the eastern tropical South Pacific. Environ. Microbiol. Rep., 2(6), 728–738.
Abstract: P>The eastern tropical Pacific Ocean holds two of the main oceanic oxygen minimum zones of the global ocean. The presence of an oxygen-depleted layer at intermediate depths, which also impinges on the seafloor and in some cases the euphotic zone, plays a significant role in structuring both pelagic and benthic communities, and also in the vertical partitioning of microbial assemblages. Here, we assessed the genetic diversity and distribution of natural populations of the cyanobacteria Prochlorococcus and Synechococcus within oxic and suboxic waters of the eastern tropical Pacific using cloning and sequencing, and terminal restriction fragment length polymorphism (T-RFLP) analyses applied to the 16S-23S rRNA internal transcribed spacer region. With the T-RFLP approach we could discriminate 19 cyanobacterial clades, of which 18 were present in the study region. Synechococcus was more abundant in the surface oxic waters of the eastern South Pacific, while Prochlorococcus dominated the subsurface low-oxygen waters. Two of the dominant clades in the oxygen-deficient waters belong to novel and yet uncultivated lineages of low-light adapted Prochlorococcus.
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Lavin, P., Henriquez-Castillo, C., Yong, S. T., Valenzuela-Heredia, D., Oses, R., Frez, K., et al. (2021). Draft Genome Sequence of Antarctic Psychrotroph Streptomyces fildesensis Strain INACH3013, Isolated from King George Island Soil. Microbiol. Resour. Ann., 10(5), e01453–20.
Abstract: The draft genome sequence of Streptomyces fildesensis strain INACH3013, a psychrotrophic bacterium isolated from Northwest Antarctic soil, was reported. The genome sequence totaling 9,306,785 bp resulted from 122 contigs characterized by a GC content of 70.55%.
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Valenzuela-Heredia, D., Henriquez-Castillo, C., Donoso, R., Lavin, P., Pavlov, M. S., Franchi, O., et al. (2020). Complete Genome Sequence of Pseudomonas chilensis Strain ABC1, Isolated from Soil. Microbiol. Resour. Ann., 9(39), 2 pp.
Abstract: Here, we report the complete genome sequence of Pseudomonas chilensis strain ABC1, which was isolated from a soil interstitial water sample collected at the University Adolfo Ibanez, Valparaiso, Chile. We assembled PacBio reads into a single closed contig with 209x mean coverage, yielding a 4,035,896-bp sequence with 62% GC content and 3,555 predicted genes.
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Valenzuela-Heredia, D., Henriquez-Castillo, C., Donoso, R., Lavin, P., Ulloa, O., Ringel, M. T., et al. (2021). An unusual overrepresentation of genetic factors related to iron homeostasis in the genome of the fluorescent Pseudomonas sp. ABC1. Microb. Biotechnol., 14(3), 1060–1072.
Abstract: Members of the genus Pseudomonas inhabit diverse environments, such as soil, water, plants and humans. The variability of habitats is reflected in the diversity of the structure and composition of their genomes. This cosmopolitan bacterial genus includes species of biotechnological, medical and environmental importance. In this study, we report on the most relevant genomic characteristics of Pseudomonas sp. strain ABC1, a siderophore-producing fluorescent strain recently isolated from soil. Phylogenomic analyses revealed that this strain corresponds to a novel species forming a sister clade of the recently proposed Pseudomonas kirkiae. The genomic information reveals an overrepresented repertoire of mechanisms to hoard iron when compared to related strains, including a high representation of fecI-fecR family genes related to iron regulation and acquisition. The genome of the Pseudomonas sp. ABC1 contains the genes for non-ribosomal peptide synthetases (NRPSs) of a novel putative Azotobacter-related pyoverdine-type siderophore, a yersiniabactin-type siderophore and an antimicrobial betalactone; the last two are found only in a limited number of Pseudomonas genomes. Strain ABC1 can produce siderophores in a low-cost medium, and the supernatants from cultures of this strain promote plant growth, highlighting their biotechnological potential as a sustainable industrial microorganism.
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