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Gnanasekaran, C., Govindan, R., Chelliah, C. K., Govindan, R., Ranganathan, P., Muthuchamy, M., et al. (2023). Isolation and molecular detection of endophytic actinomycetes Nocardiopsis dassonvillei DMS 1 (MH900216) from marine sea grasses with bacterial inactivation. Biocatal. Agric. Biotechnol., 54, 102938.
Abstract: In recent years, new antibiotics have been discovered around the world in order to inhibit multi drug resistant (MDR) pathogens. To overcome this problem, marine actinomycetes are an alternative choice for producing new bioactive compounds that inhibit MDR bacteria. The typical endophytic actinomycete (EA) Nocardiopsis dassonvillei (N. dassonvillei) DMS 1 (MH900216) was isolated from marine Sea grasses by surface sterilization method. After surface sterilization, it was confirmed that the pure, dry, white-colored spore producing colonies emerged from the internal tissue of the Sea grasses. The crude extract of N. dassonvillei DMS 1 (MH900216) demonstrated 8 and 10-mm zones of inhibition against A. baumannii and K. pneumoniae, respectively. The composition of N. dassonvillei DMS 1 (MH900216) with potential anti-bacterial properties was studied by GC-MS analysis and exhibited 22 chemical compounds. Subsequently, the molecular identification and phylogenetic construction of the isolated EA strain was confirmed as N. dassonvillei DMS 1 (MH900216). The liquid-liquid extraction of the compound demonstrated 24-and 26-mm zones of inhibition against A. baumannii and K. pneumoniae, respectively. Furthermore, the purified crude compound demonstrated 92% and 94% cell death against A. baumannii and K. pneumoniae, respectively, at a minimum inhibitory concentration of 500 mu g/mL. Overall, the present study demonstrated the antibacterial properties of the EA N. dassonvillei DMS 1 (MH900216) isolated from Sea grasses and their importance as alternative sources for discovering new antibiotics to inhibit MDR bacteria.
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Rajivgandhi, G., Chelliah, C. K., Murugan, M., Ramachandran, G., Chackaravarthi, G., Maruthupandy, M., et al. (2023). Discovery of secondary metabolites from Avicennia marina to inhibit the anti-oxidant and anti-biofilm activities of biofilm forming bacteria. J. King Saud Univ. Sci., 36(1), 102979.
Abstract: The present study is concentrated on screening of phytochemical and bioactive compounds from marine mangrove plant of Avicennia marina (A. marina). The plant extract was purified by soxhlet extraction process, and the available phytochemicals, bioactive compounds and other organic compounds were confirmed by LC-MS analysis. Subsequently, the antioxidant and DPPH scavenging activities of the A. marina extract was shown 79 % and 88 % at 500 mu g/mL concentrations were observed respectively. Further, the antimicrobial efficacy of A. marina extract was shown 22 and 26 mm and 18 and 24 mm zones of inhibition against K. pneumoniae, A. baumannii, S. aureus and Enterococcus sp. at 500 mu g/ml concentrations by agar well diffusion method. In addition, the liquid medium based bacterial inactivation was clearly observed with 78 %, 89 %, 81 % and 90 % at 500 mu g/ml concentration. Furthermore, the biofilm inhibition concentration and exopolysaccharide degradation effect of A. marina extract indicated that the bacteria were completely arrested and the inhibition range of 92 %, 88 % 84 % and 90 % and 89 %, 94 %, 90 % and 86 % were observed respectively. Finally, the result evidences were clearly indicated that the mangrove plant A. marina was excellent antimicrobial inhibitor and also future drug discovery plant to fight against some important multi drug resistant pathogens.
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