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Collins, C. J., Vivanco, J. F., Sokn, S. A., Williams, B. O., Burgers, T. A., & Ploeg, H. L. (2015). Fracture healing in mice lacking Pten in osteoblasts: a micro-computed tomography image-based analysis of the mechanical properties of the femur. J. Biomech., 48(2), 310–317.
Abstract: In the United States, approximately eight million osseous fractures are reported annually, of which 5-10% fail to create a bony union. Osteoblast-specific deletion of the gene Pten in mice has been found to stimulate bone growth and accelerate fracture healing. Healing rates at four weeks increased in femurs from Pten osteoblast conditional knock-out mice (Pten-CKO) compared to wild-type mice (WT) of the same genetic strain as measured by an increase in mechanical stiffness and failure load in four-point bending tests. Preceding mechanical testing, each femur was imaged using a Skyscan 1172 micro-computed tomography (mu CT) scanner (Skyscan, Kontich, Belgium). The present study used μCT image-based analysis to test the hypothesis that the increased femoral fracture force and stiffness in Pten-CKO were due to greater section properties with the same effective material properties as that of the WT. The second moment of area and section modulus were computed in ImageJ 1.46 (National Institutes of Health) and used to predict the effective flexural modulus and the stress at failure for fourteen pairs of intact and callus WT and twelve pairs of intact and callus Pten-CKO femurs. For callus and intact femurs, the failure stress and tissue mineral density of the Pten-CKO and WT were not different; however, the section properties of the Pten-CKO were more than twice as large 28 days post-fracture. It was therefore concluded, when the gene Pten was conditionally knocked-out in osteoblasts, the resulting increased bending stiffness and force to fracture were due to increased section properties. (C) 2014 Elsevier Ltd. All rights reserved.
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Vivanco, J. F., Burgers, T. A., Garcia-Rodriguez, S., Crookshank, M., Kunz, M., MacIntyre, N. J., et al. (2014). Estimating the density of femoral head trabecular bone from hip fracture patients using computed tomography scan data. Proc. Inst. Mech. Eng. Part H-J. Eng. Med., 228(6), 616–626.
Abstract: The purpose of this study was to compare computed tomography density (rho(CT)) obtained using typical clinical computed tomography scan parameters to ash density (rho(ash)), for the prediction of densities of femoral head trabecular bone from hip fracture patients. An experimental study was conducted to investigate the relationships between rho(ash) and rho(CT) and between each of these densities and rho(bulk) and rho(dry). Seven human femoral heads from hip fracture patients were computed tomography-scanned ex vivo, and 76 cylindrical trabecular bone specimens were collected. Computed tomography density was computed from computed tomography images by using a calibration Hounsfield units-based equation, whereas rho(bulk), rho(dry) and rho(ash) were determined experimentally. A large variation was found in the mean Hounsfield units of the bone cores (HUcore) with a constant bias from rho(CT) to rho(ash) of 42.5 mg/cm(3). Computed tomography and ash densities were linearly correlated (R-2 = 0.55, p < 0.001). It was demonstrated that rho(ash) provided a good estimate of rho(bulk) (R-2 = 0.78, p < 0.001) and is a strong predictor of rho(dry) (R-2 = 0.99, p < 0.001). In addition, the rho(CT) was linearly related to rho(bulk) (R-2 = 0.43, p < 0.001) and rho(dry) (R-2 = 0.56, p < 0.001). In conclusion, mineral density was an appropriate predictor of rho(bulk) and rho(dry), and rho(CT) was not a surrogate for rho(ash). There were linear relationships between rho(CT) and physical densities; however, following the experimental protocols of this study to determine rho(CT), considerable scatter was present in the rho(CT) relationships.
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