Abstract: Additive manufacturing encompasses a plethora of techniques to manufacture structures from a computational model. Among them, fused filament fabrication (FFF) relies on heating thermoplastics to their fusion point and extruding the material through a nozzle in a controlled pattern. FFF is a suitable technique for tissue engineering, given that allows the fabrication of 3D-scaffolds, which are utilized for tissue regeneration purposes. The objective of this study is to assess a low-cost/open-source 3D printer (In-House), by manufacturing both solid and porous samples with relevant microarchitecture in the physiological range (100?500 ?m pore size), using an equivalent commercial counterpart for comparison. For this, compressive tests in solid and porous scaffolds manufactured in both printers were performed, comparing the results with finite element analysis (FEA) models. Additionally, a microarchitectural analysis was done in samples from both printers, comparing the measurements of both pore size and porosity to their corresponding computer-aided design (CAD) models. Moreover, a preliminary biological assessment was performed using scaffolds from our In-House printer, measuring cell adhesion efficiency. Finally, Fourier transform infrared spectroscopy ? attenuated total reflectance (FTIR?ATR) was performed to evaluate chemical changes in the material (polylactic acid) after fabrication in each printer. The results show that the In-House printer achieved generally better mechanical behavior and resolution capacity than its commercial counterpart, by comparing with their FEA and CAD models, respectively. Moreover, a preliminary biological assessment indicates the feasibility of the In-House printer to be used in tissue engineering applications. The results also show the influence of pore geometry on mechanical properties of 3D-scaffolds and demonstrate that properties such as the apparent elastic modulus (Eapp) can be controlled in 3D-printed scaffolds.

Abstract: This study deals with industrial trends in tunnelling by drill and blast (D&B). We perform a statistical analysis of accumulated experience from the 1950s to the modern day to provide advice for proper project management in tunnel driving. The basis of the study is a wide database of tunnel blast schemes. This database is made of excavation parameters, and considers two main families of blasts: with parallel hole cuts and with inclined hole cuts. Such parameters are analysed by means of statistical regression. Correlations are shown. We present a general curve of correlation between tunnel sections and specific drilling and specific explosive consumption. We show how pull efficiency cannot be correlated to a single parameter, and how tunnelling by D&B needs to be treated as a complex system. Finally, we propose a method for quantifying and classifying the difficulty of tunnelling. The deviation of specific drilling (SD) from industrial average trend is used as an indicator of difficulty: easier when SD is lower than average, and more difficult when SD is higher than average. We show how such deviation can be preliminarily associated with lithotypes. This provides to designers and cost estimators a tool of a first approximation for D&B cost prediction at the pre-feasability and feasibility stages of a tunnelling project. (C) 2016 Elsevier Ltd. All rights reserved.

Abstract: This article investigates the feasibility of achieving higher performance from ground-source heat-pumps (GSHP) in space heating mode through the use of solar thermal collectors. A novel simulation tool for solar-assisted ground-source heat-pumps (SGSHP) is presented with an analysis of the influence of solar collectors on the improvement of heat pump performance. Solar radiation and climate temperature data of 19 European cities were used to perform simulations of SGSHP and GSHP systems considering a typical residential house. Overall performance coefficients (COPsys) varied from northern to southern locations between 4.4 and 5.8 for SGSHP and between 4.3 and 5.1 for GSHP. Results show that solar collectors coupling has more impact on performance improvement in regions that benefit from higher irradiance. However, greater running cost savings are achieved in milder climate conditions. Both heat-pump systems are able to effectively contribute to carbon footprint reductions for residential buildings, especially in countries where fossil fuels are the primary source of electricity generation. SGSHP payback periods are found between 8.5 and 23 years from northern to southern localities, making such heating system an economic heating option. SGSHPs are best suited for high irradiance and cool climate locations such as the mountainous regions in southern Europe. (C) 2015 Elsevier Ltd. All rights reserved.

Abstract: This research compares the building energy consumption and the photovoltaic economic analysis between residential buildings in Santiago de Chile and Santo Domingo of the Dominican Republic. The methodology considered thermal simulation, sizing of a solar PV system, an economic analysis and CO2 emissions given the solar resources of both countries. A scenario where the constructive systems are switched between the countries was also analyzed. A comparison of the energy performances of the houses exposed to other climate conditions. Results show that housing in Santiago de Chile required less energy than housing in Santo Domingo due to the fact that the thermal transmittance of the enclosures of the Chilean housing has better thermal behavior, compared to the materials of the Dominican housing. Dominican houses need a higher amount of electricity for air cooling due to the high temperatures in the tropic. Meanwhile, Chilean countries requires a higher amount of gas for heating purposes. The Dominican Republic lacks thermal regulation for construction material, and applying Chilean standards in Dominican houses, helped to lower the yearly electricity demand by 19%. Dominican constructions materials improvement could have an important impact in the country's overall goal to lower CO2 emission and in-house energy savings. The economic analysis showed that the Dominican Republic renewable energies incentives contribute to the development of very attractive PV projects, meanwhile in Chile, the use of net metering instead of net billing could increase by 11 times the net present value of PV projects.

Abstract: Asphalt self-healing by encapsulated rejuvenating agents is considered a revolutionary technology for the autonomic crack-healing of aged asphalt pavements. This paper aims to explore the use of Bio-Oil (BO) obtained from liquefied agricultural biomass waste as a bio-based encapsulated rejuvenating agent for self-healing of bituminous materials. Novel BO capsules were synthesized using two simple dripping methods through dropping funnel and syringe pump devices, where the BO agent was microencapsulated by external ionic gelation in a biopolymer matrix of sodium alginate. Size, surface aspect, and elemental composition of the BO capsules were characterized by optical and scanning electron microscopy and energy-dispersive X-ray spectroscopy. Thermal stability and chemical properties of BO capsules and their components were assessed through thermogravimetric analysis (TGA-DTG) and Fourier-Transform Infrared spectroscopy (FTIR-ATR). The mechanical behavior of the capsules was evaluated by compressive and low-load micro-indentation tests. The self-healing efficiency over time of BO as a rejuvenating agent in cracked bitumen samples was quantified by fluorescence microscopy. Main results showed that the BO capsules presented an adequate morphology for the asphalt self-healing application, with good thermal stability and physical-chemical properties. It was also proven that the BO can diffuse in the bitumen reducing the viscosity and consequently self-healing the open microcracks.

Abstract: Water-use efficiency (WUE) is a quantitative measurement of biomass produced per volume of water transpired by a plant. WUE is an important physiological trait for drought response to mitigate the water deficiency. In this work, a cisgenic construction from Citrus sinensis was developed and its function in the improvement of WUE was evaluated in Arabidopsis. Sequences of the CsMYB61 coding region, a transcription factor implicated in the closure of stomata, together with a putative stomata-specific promoter from CsMYB.1.5, were identified and cloned. The protein encoded in the CsMYB61 locus harbors domains and motifs characteristic of MYB61 proteins. In addition, a 1.2 kb promoter region of the gene CsMYB15 (pCsMYB15) containing regulatory elements for expression in guard cells and in response to Abscisic Acid (ABA) and light was isolated. In Arabidopsis, pCsMYB15 directs the expression of the reporter gene GUS in stomata in the presence of light. In addition, transgenic lines expressing the CsMYB61 coding region under transcriptional control of pCsMYB15 have a normal phenotype under in vitro and greenhouse conditions. These transgenic lines exhibited a smaller opening of the stomata pore, lower stomatal conductance and respiration rate, enhanced sensitivity to exogenous ABA, and high drought stress tolerance. Our results indicate that stomata-specific expression of CsMYB61 enhances water use efficiency under drought conditions in Arabidospis.

Abstract: To reduce the energy consumption in buildings, there is a demand for tools that identify significant parameters of energy performance. The work presents the development and validation of a simulation model, called MEEDI, and graphical figures for the parametric sensitivity investigation of energy performance in different climates in Chile. The MEEDI is based on the ISO 13790 monthly calculation method of building energy use with two improved procedures for the calculation of the heat transfer through the floor and the solar heat gains. The graphical figures illustrate the effects of climate conditions, envelope components and window size and orientation on the energy consumption. The MEEDI program can contribute to find the best solution to increase energy efficiency in residential buildings. It can be adapted for various parameters, making it useful for future projects. The economic viability of specific measures for building envelope materials was analysed. Payback periods range from 5 to 27 years depending on the location and energy scenario. The study illustrates how building design decisions can have a significant impact on final energy performance. With simple envelope components modification, valuable energy gains and carbon emission reductions can be achieved in a cost-effective manner in Chile.

Abstract: In many countries, bus operators are private companies whose service has been leased by government agencies. These agencies develop service compliance indices or measures to keep track of factors such as passenger satisfaction, frequency, and regularity but do not necessarily include the objectives of the operators in the assessment. In this paper, we used slack-based measure data envelopment analysis (SBM) to investigate whether it is possible for a bus operator to be efficient (from a private perspective) and match required standards of frequency and regularity. In doing so, data collected from two major bus operators in Santiago, Chile has been used comprising 99 services. The results show that when private objectives, namely revenues, are included in the analysis, bus operators do not necessarily seek to improve the regularity of their service. Moreover, it was found that some bus services are on the efficient frontier while keeping low performance measure standards. Using the shadow prices of the models, it was also found that improving the performance measures will be hard for many bus services unless there is a significant change in factors that are not under control of the operators (i.e., number of stops, length of the route, etc.). This shows the difficulty of correctly aligning the private objectives of operators with agencies' objectives.