Abstract: In this work, clustering analysis of two partial nitritation-anammox (PN-A) moving bed biofilm reactors (MBBR) containing different types of carrier material was explored for the identification of patterns and operational conditions that may benefit process performance. The systems ran for two years under fluctuations of temperature and organic matter. Ex situ batch activity tests were performed every other week during the operation of these reactors. These datasets and the parameters, which were monitored online and in the laboratory, were combined and analyzed applying clustering analysis to identify non-obvious information regarding the performance of the systems. The initial results were consistent with the literature and from an operational perspective, which allowed the parameters to be explored further. The new information revealed that the oxidation reduction potential (ORP) and the anaerobic ammonium oxidizing bacteria (AnAOB) activity correlated well. ORP also dropped when the reactors were exposed to real wastewater (presence of organic matter). Moreover, operating conditions during nitrite accumulation were identified through clustering, and also revealed inhibition of anammox bacteria already at low nitrite concentrations.

Abstract: The usual method to determine the relative sensitivity curve for total-reflection X-ray fluorescence (TXRF) uses multielemental solutions, which may be purchased or prepared in the laboratory. In the former case, the accuracy and precision of the concentrations are certified by the provider, while in the latter, the experience of the technical staff determines the analytical quality. These procedures are costly and the quality of the solutions cannot be easily verified. The goal of this work was to use pure crystalline salts containing two elements that may be quantified by TXRF for the calibration of the spectrometer. The analysis of these samples along with a mathematical procedure assures good precision of the results. The reported method is economically efficient, simple, and eliminates the uncertainties of the element concentration in the samples produced by the standard methods, thereby improving the quality of TXRF results.

Abstract: A reply to the previous article commenting on non-geodesical propagation of electromagnetic fields on gravitational backgrounds and the eikonal limit are presented.

Abstract: Irrigation scheduling based on soil water content (Ow) sensors requires that Ow be maintained within a range (management lines) that is optimal for plant growth. The lower limit or “breaking point ” is determined following the soil water content dynamics on the transition of a rapid rate of depletion to a slower, under similar reference evapotranspiration. Although this criterion is practical, its implementation should be validated with plant water status measurement that contemplate weather condition, such as stem water potential “non-stressed ” baseline (Tx as a function of vapor-pressure deficit (VPD) in Ow conditions that do not limit yield). A study was con-ducted on a mature cv. 'Hass' avocado orchard in Central Chile during two seasons. There were 5 irrigation treatments: T1, Control; T2 and T3 with 29% less and 25% more of what was applied in T1, respectively; T4 and T5 same as Control until first and second fruit drop abscission, respectively, and then with 29% less. T1 trees were irrigated using a continuous frequency domain reflectometry (FDR) probe to maintain the root zone be-tween field capacity and the breaking point. There was biweekly monitoring of the Ow prior to irrigation, Tx and VPD. The Tx decline proportional to the intensity and the timing of water restriction; however, no treatment affected the crop load in either season. T2 did not show significant detrimental in fruit size, production and maturation, despite that frequently reached water content levels at the limit of the breaking point, and showed lower levels of stem water potential than Control, being the treatment with the highest water productivity. The results confirm that breaking point is an effective criterion to establish irrigation management. Additionally, when comparing the baseline for our non-stressed trees with a baseline from full irrigation treatments obtained from the literature, 30% water savings were achieved.

Abstract: Human performance enhancing drugs (PEDs), frequently used in sport competitions, are strictly prohibited by the World Anti-Doping Agency (WADA). Biological samples collected from ath-letes and regular patients are continuously tested regarding the identification and/or quantification of the banned substances. Current work is focused on the application of a new analytical method, molecularly imprinted nanoparticles (nanoMIPs), to detect and determine concentrations of certain prohibited drugs, such as B-blockers, in water and human urine samples. These medications are used in the treatment of cardiovascular conditions, negative effects of adrenaline (helping to relief stress), and hypertension (slowing down the pulse and softening the arteries). They can also significantly increase muscle relaxation and improve heart efficiency. The new method of the detection and quantification of B-blockers is based on synthesis, characterization, and implementation of nanoMIPs (so-called plastic antibodies). It offers numerous advantages over the traditional methods, including high binding capacity, affinity, and selectivity for target molecules. Additionally, the whole process is less complicated, cheaper, and better controlled. The size and shape of the nanoMIPs is evaluated by dynamic light scattering (DLS) and transmission electron microscope (TEM). The affinity and selectivity of the nanoparticles are investigated by competitive pseudo enzyme-linked immunosorbent assay (pseudo-ELISA) similar to common immunoassays employing natural antibodies. To provide reliable results towards either doping detection or therapeutic monitoring using the minimal invasive method, the qualitative and quantitative analysis of these drugs is performed in water and human urine samples. It is demonstrated that the assay can detect B-blockers in water within the linear range 1 nmolmiddotL(-1)-1 mmolmiddotL(-1) for atenolol with the detection limit 50.6 ng mL(-1), and the linear range 1 mmolmiddotL(-1)-10 mmolmiddotL(-1) for labetalol with the detection limit of 90.5 ngmiddotmL(-1). In human urine samples, the linear range is recorded in the concentration range 0.1 mmolmiddotL(-1)-10 nmolmiddotL(-1) for atenolol and 1 mmolmiddotL(-1)-10 nmolmiddotL(-1) for labetalol with a detection limit of 61.0 ngmiddotmL(-1)for atenolol and 99.4 ngmiddotmL(-1) for labetalol.

Abstract: Viral infections in salmonids represent an ongoing challenge for the aquaculture industry. Two RNA viruses, the infectious pancreatic necrosis virus (IPNV) and the infectious salmon anemia virus (ISAV), have become a latent risk without healing therapies available for either. In this context, antiviral peptides emerge as effective and relatively safe therapeutic molecules. Based on in silico analysis of VP2 protein from IPNV and the RNA-dependent RNA polymerase from ISAV, a set of peptides was designed and were chemically synthesized to block selected key events in their corresponding infectivity processes. The peptides were tested in fish cell lines in vitro, and four were selected for decreasing the viral load: peptide GIM182 for IPNV, and peptides GIM535, GIM538 and GIM539 for ISAV. In vivo tests with the IPNV GIM 182 peptide were carried out using Salmo salar fish, showing a significant decrease of viral load, and proving the safety of the peptide for fish. The results indicate that the use of peptides as antiviral agents in disease control might be a viable alternative to explore in aquaculture.`

Abstract: Electric motors are among the most widely used electro-mechanical systems in the mining industry in Chile. As they are physically present in virtually all phases of the production process, they must be regularly inspected. In order to maintain a maximum level of operability and to avoid the occurrence of catastrophic failures and unscheduled stoppages, regular, planned reviews to measure the internal parameters of their components are performed manually. This part of the process presents serious shortcomings, such as significant inspection times, information input errors and, in some cases, measurement errors resulting from the technical difficulty of physically taking the measurement. This research presents the design, development and evaluation of a new sensor to support the inspection of brush-holder spring tensions as well as the integration of a digital gauge to control other critical dimensions of the brush-holder mounting. Its purpose is to provide the mining industry with a new system and a fully automated inspection protocol to ease maintenance and control tasks, particularly focusing on reducing inspection times. These products are expected to increase the equipment operating life, as well as delivering a maintenance report of the motor's key parameters which directly benefits the Key Performance Indicators (KPI) of mining management

Abstract: We propose a representation of suboptimal investment behavior based on the stochastic discount factor (SDF) paradigm. Suboptimal investment behavior is rationalized as being the investor's optimal decision under a wrong SDF, while wealth trajectories and budget constraints are based on the true SDF. We develop a novel Monte Carlo simulation approach to compute the welfare costs for this suboptimal behavior. We study the suboptimal portfolio choice under CRRA preferences using two financial market models. The Monte Carlo simulation delivers comparable welfare losses to those computed in the original studies, which are based on partial differential equations (PDE) and – finite-difference schemes.

Abstract: Chile is expecting a 5.4% growth in energy consumption per year until 2030, requiring new and better solutions for the upward trend of its electricity demand. This state leads to select and study one of the potential alternatives for electricity generation: concentrated solar power (CSP) plants. Such renewable technology found in Chile a very favorable condition. Recent researches indicate Atacama Desert as one of the best regions for solar energy worldwide, having an average radiation higher than in places where CSP plants are currently implemented, e.g. Spain and USA. The aim of this study is to present an analysis of levelized energy cost (LEC) for different power capacities of CSP plants placed in distinct locations in northern Chile. The results showed that CSP plants can be implemented in Atacama Desert with LECs around 19 (sic)US$/kWh when a gas-fired backup and thermal energy storage (TES) systems are fitted. This value increases to approximately 28 (sic)US$/kWh if there is no backup system. (C) 2012 Elsevier Ltd. All rights reserved.

Abstract: The self-sufficient cytochrome P450 RhF and its homologues belonging to the CYP116B subfamily have attracted considerable attention due to the potential for biotechnological applications based in their ability to catalyse an array of challenging oxidative reactions without requiring additional protein partners. In this work, we showed for the first time that a CYP116B self-sufficient cytochrome P450 encoded by the ohpA gene harboured by Cupriavidus pinatubonensis JMP134, a beta-proteobacterium model for biodegradative pathways, catalyses the conversion of 2-hydroxyphenylacetic acid (2-HPA) into homogentisate. Mutational analysis and HPLC metabolite detection in strain JMP134 showed that 2-HPA is degraded through the well-known homogentisate pathway requiring a 2-HPA 5-hydroxylase activity provided by OhpA, which was additionally supported by heterologous expression and enzyme assays. The ohpA gene belongs to an operon including also ohpT, coding for a substrate-binding subunit of a putative transporter, whose expression is driven by an inducible promoter responsive to 2-HPA in presence of a predicted OhpR transcriptional regulator. OhpA homologues can be found in several genera belonging to Actinobacteria and alpha-, beta- and gamma-proteobacteria lineages indicating a widespread distribution of 2-HPA catabolism via homogentisate route. These results provide first time evidence for the natural function of members of the CYP116B self-sufficient oxygenases and represent a significant input to support novel kinetic and structural studies to develop cytochrome P450-based biocatalytic processes.