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de la Guardia, A. R. H., Ugalde, M. B., Lobos-Diaz, V., Romero-Romero, J. L., Meyer-Regueiro, C., Inostroza-Blancheteau, C., et al. (2021). Isolation and molecular characterization of MYB60 in Solanum lycopersicum. Mol. Biol. Rep., 48(2), 1579–1587.
Abstract: Stomatal closure is a common adaptation response of plants to the onset of drought condition and its regulation is controlled by transcription factors. MYB60, a transcription factor involved in the regulation of light-induced stomatal opening, has been characterized in arabidopsis and grapevine. In this work, we studied the role of MYB60 homolog SIMYB60 in tomato plants. We identified, isolated, and sequenced the SIMYB60 coding sequence, and found domains and motifs characteristic of other MYB60 proteins. We determined that SlMYB60 is mainly expressed in leaves, and its expression is repressed by abscisic acid. Next, we isolated a putative promoter region containing regulatory elements responsible for guard cell expression and other putative regulatory elements related to ABA repression and vascular tissue expression. Protein localization assays demonstrated that SlMYB60 localizes to the nucleus. Finally, SlMYB60 is able to complement the mutant phenotype of atmyb60-1 in Arabidopsis. Together, these results indicate that SlMYB60 is the homologous gene in tomato and potentially offer a molecular target to improve crops.
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Luengwilai, K., Yu, J. W., Jimenez, R. C., Thitisaksakul, M., Vega, A., Dong, S. Y., et al. (2022). Ectopic Expression of Arabidopsis thaliana zDof1.3 in Tomato (Solanum lycopersicum L.) Is Associated with Improved Greenhouse Productivity and Enhanced Carbon and Nitrogen Use. Int. J. Mol. Sci., 23(19), 11229.
Abstract: A large collection of transgenic tomato lines, each ectopically expressing a different Arabidopsis thaliana transcription factor, was screened for variants with alterations in leaf starch. Such lines may be affected in carbon partitioning, and in allocation to the sinks. We focused on 'L4080', which harbored an A. thaliana zDof (DNA-binding one zinc finger) isoform 1.3 (AtzDof1.3) gene, and which had a 2-4-fold higher starch-to-sucrose ratio in source leaves over the diel (p < 0.05). Our aim was to determine whether there were associated effects on productivity. L4080 plants were altered in nitrogen (N) and carbon (C) metabolism. The N-to-C ratio was higher in six-week-old L4080, and when treated with 1/10 N, L4080 growth was less inhibited compared to the wild-type and this was accompanied by faster root elongation (p < 0.05). The six-week-old L4080 acquired 42% more dry matter at 720 ppm CO2, compared to ambient CO2 (p < 0.05), while the wild-type (WT) remained unchanged. GC-MS-TOF data showed that L4080 source leaves were enriched in amino acids compared to the WT, and at 49 DPA, fruit had 25% greater mass, higher sucrose, and increased yield (25%; p < 0.05) compared to the WT. An Affymetrix cDNA array analysis suggested that only 0.39% of the 9000 cDNAs were altered by 1.5-fold (p < 0.01) in L4080 source leaves. C-14-labeling of fruit disks identified potential differences in 14-DPA fruit metabolism suggesting that post-transcriptional regulation was important. We conclude that AtzDof1.3 and the germplasm derived therefrom, should be investigated for their 'climate-change adaptive' potential.
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Ruivo, E. L. P., Montalva-Medel, M., de Oliveira, P. P. B., & Perrot, K. (2018). Characterisation of the elementary cellular automata in terms of their maximum sensitivity to all possible asynchronous updates. Chaos Solitons Fractals, 113, 209–220.
Abstract: Cellular automata are fully-discrete dynamical systems with global behaviour depending upon their locally specified state transitions. They have been extensively studied as models of complex systems as well as objects of mathematical and computational interest. Classically, the local rule of a cellular automaton is iterated synchronously over the entire configuration. However, the question of how asynchronous updates change the behaviour of a cellular automaton has become a major issue in recent years. Here, we analyse the elementary cellular automata rule space in terms of how many different one-step trajectories a rule would entail when taking into account all possible deterministic ways of updating the rule, for one time step, over all possible initial configurations. More precisely, we provide a characterisation of the elementary cellular automata, by means of their one-step maximum sensitivity to all possible update schedules, that is, the property that any change in the update schedule causes the rule's one-step trajectories also to change after one iteration. Although the one-step maximum sensitivity does not imply that the remainder of the time-evolutions will be distinct, it is a necessary condition for that. (C) 2018 Elsevier Ltd. All rights reserved.
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Viveros, M. F. A., Inostroza-Blancheteau, C., Timmermann, T., Gonzalez, M., & Arce-Johnson, P. (2013). Overexpression of GlyI and GlyII genes in transgenic tomato (Solanum lycopersicum Mill.) plants confers salt tolerance by decreasing oxidative stress. Mol. Biol. Rep., 40(4), 3281–3290.
Abstract: The glyoxalase system plays an important role in various physiological processes in plants, including salt stress tolerance. We report the effects of overexpressing glyoxalase I and glyoxalase II genes in transgenic tomato (Solanum lycopersicum Mill.) cv. Ailsa Craig. Stable expression of both transgenes was detected in the transformed tomato plants under salt stress. The transgenic lines overexpressing GlyI and GlyII under a high NaCl concentration (800 mM) showed reduced lipid peroxidation and the production of H2O2 in leaf tissues. A greater decrease in the chlorophyll a+b content in wild-type (WT) compared with transgenic lines was also observed. These results suggest that the over expression of two genes, GlyI and GlyII, may enhance salt stress tolerance by decreasing oxidative stress in transformed tomato plants. This work will help our understanding of the putative role of the glyoxalase system in the tolerance to abiotic stress in tomato plants.
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